Amino Acids

, Volume 44, Issue 5, pp 1247–1251 | Cite as

Interaction proteomics of the AMPA receptor: towards identification of receptor sub-complexes

Invited review
First Online:
Received:
Accepted:

Abstract

AMPA receptors (AMPAR) are the main ligand-gated ion channels responsible for the fast excitatory synaptic transmission in the mammalian brain. Whereas a number of proteins that interact with AMPAR are known to be involved in the trafficking and localization of the receptor and/or the regulation of receptor channel properties, the protein composition of the AMPAR supra-complexes are largely unclear. Recent interaction proteomics report the presence of up to 34 proteins as high-confidence constituents of the AMPAR. It was proposed that the inner core of the receptor complex consists of the GluA tetramer and four auxiliary proteins comprising transmembrane AMPA receptor regulatory proteins and/or cornichons. The other AMPAR interactors, present in lower amount, may form the outer shell of the AMPAR with a range in size and variability.

Keywords

Synapse Excitatory neurotransmission AMPA receptor Protein complex Proteomics 

Abbreviations

AMPAR

AMPA receptor

BN-PAGE

Blue-native polyacrylamide gel electrophoresis

ESI

Electrospray

IP

Immuno-precipitation

MALDI

Matrix-assisted laser desorption ionization

MS

Mass spectrometry

TARP

Transmembrane AMPA receptor regulatory protein

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Notes

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  1. 1.Department of Molecular and Cellular Neurobiology, Faculty of Earth and Life Sciences, Center for Neurogenomics and Cognitive Research, Neuroscience Campus AmsterdamVU UniversityAmsterdamThe Netherlands

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