Amino Acids

, Volume 44, Issue 3, pp 1061–1071 | Cite as

A cysteine in the repetitive domain of a high-molecular-weight glutenin subunit interferes with the mixing properties of wheat dough

  • Xin Gao
  • Qisen Zhang
  • Marcus P. Newberry
  • Ken J. Chalmers
  • Diane E. Mather
Original Article

Abstract

The quality of wheat (Triticum aestivum L.) for making bread is largely due to the strength and extensibility of wheat dough, which in turn is due to the properties of polymeric glutenin. Polymeric glutenin consists of high- and low-molecular-weight glutenin protein subunits linked by disulphide bonds between cysteine residues. Glutenin subunits differ in their effects on dough mixing properties. The research presented here investigated the effect of a specific, recently discovered, glutenin subunit on dough mixing properties. This subunit, Bx7.1, is unusual in that it has a cysteine in its repetitive domain. With site-directed mutagenesis of the gene encoding Bx7.1, a guanine in the repetitive domain was replaced by an adenine, to provide a mutant gene encoding a subunit (MutBx7.1) in which the repetitive-domain cysteine was replaced by a tyrosine residue. Bx7.1, MutBx7.1 and other Bx-type glutenin subunits were heterologously expressed in Escherichia coli and purified. This made it possible to incorporate each individual subunit into wheat flour and evaluate the effect of the cysteine residue on dough properties. The Bx7.1 subunit affected dough mixing properties differently from the other subunits. These differences are due to the extra cysteine residue, which may interfere with glutenin polymerisation through cross-linkage within the Bx7.1 subunit, causing this subunit to act as a chain terminator.

Keywords

Cysteine Dough properties Glutenin polymerisation High-molecular-weight glutenin Site-directed mutagenesis Wheat 

Notes

Acknowledgments

The authors thank Mr Ibrahim Kutty for assistance in the operation of the z-arm mixer and 10-g mixograph system, and the China Scholarship Council and the University of Adelaide for a scholarship awarded to the first author.

Supplementary material

726_2012_1441_MOESM1_ESM.pdf (121 kb)
Supplementary material 1 10-g mixograph mixing curves of Gabo, Glenlea, H45 and VQ0437. (PDF 121 kb)
726_2012_1441_MOESM2_ESM.pdf (345 kb)
Supplementary material 2 LC-eSI-IT mass spectrometry report on the sequence coverage of Bx7OE, Bx7.1 and MutBx7.1 subunits. (PDF 345 kb)

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Copyright information

© Springer-Verlag Wien 2012

Authors and Affiliations

  • Xin Gao
    • 1
  • Qisen Zhang
    • 1
    • 2
  • Marcus P. Newberry
    • 3
  • Ken J. Chalmers
    • 1
    • 4
  • Diane E. Mather
    • 1
    • 2
    • 4
  1. 1.School of Agriculture, Food and Wine, Waite Research InstituteThe University of AdelaideGlen OsmondAustralia
  2. 2.Australian Research Council Centre of Excellence in Plant Cell Walls, Waite Research InstituteThe University of AdelaideGlen OsmondAustralia
  3. 3.Food Futures National Research Flagship and Division of Plant IndustryCommonwealth Scientific and Industrial OrganisationCanberraAustralia
  4. 4.Australian Centre for Plant Functional GenomicsWaite Research Institute, The University of AdelaideGlen OsmondAustralia

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