Amino Acids

, Volume 44, Issue 3, pp 911–923 | Cite as

Dietary supplementation with monosodium glutamate is safe and improves growth performance in postweaning pigs

  • Reza Rezaei
  • Darrell A. Knabe
  • Carmen D. Tekwe
  • Sudath Dahanayaka
  • Martin D. Ficken
  • Susan E. Fielder
  • Sarah J. Eide
  • Sandra L. Lovering
  • Guoyao Wu
Original Article

Abstract

Dietary intake of glutamate by postweaning pigs is markedly reduced due to low feed consumption. This study was conducted to determine the safety and efficacy of dietary supplementation with monosodium glutamate (MSG) in postweaning pigs. Piglets were weaned at 21 days of age to a corn and soybean meal-based diet supplemented with 0, 0.5, 1, 2, and 4 % MSG (n = 25/group). MSG was added to the basal diet at the expense of cornstarch. At 42 days of age (21 days after weaning), blood samples (10 mL) were obtained from the jugular vein of 25 pigs/group at 1 and 4 h after feeding for hematological and clinical chemistry tests; thereafter, pigs (n = 6/group) were euthanized to obtain tissues for histopathological examinations. Feed intake was not affected by dietary supplementation with 0–2 % MSG and was 15 % lower in pigs supplemented with 4 % MSG compared with the 0 % MSG group. Compared with the control, dietary supplementation with 1, 2 and 4 % MSG dose-dependently increased plasma concentrations of glutamate, glutamine, and other amino acids (including lysine, methionine, phenylalanine and leucine), daily weight gain, and feed efficiency in postweaning pigs. At day 7 postweaning, dietary supplementation with 1–4 % MSG also increased jejunal villus height, DNA content, and antioxidative capacity. The MSG supplementation dose-dependently reduced the incidence of diarrhea during the first week after weaning. All variables in standard hematology and clinical chemistry tests, as well as gross and microscopic structures, did not differ among the five groups of pigs. These results indicate that dietary supplementation with up to 4 % MSG is safe and improves growth performance in postweaning pigs.

Keywords

Monosodium glutamate Piglet Growth Safety Efficacy 

References

  1. Bergen WG (1974) Protein synthesis in animal models. J Anim Sci 38:1079–1091PubMedGoogle Scholar
  2. Bertolo RF, Burrin DG (2008) Comparative aspects of tissue Glutamine and proline metabolism. J Nutr 138:2032S–2039SPubMedGoogle Scholar
  3. Blachier F, Boutry C, Bos C, Tome D (2009) Metabolism and functions of l-glutamate in the epithelial cells of the small and large intestines. Am J Clin Nutr 90:814S–821SPubMedCrossRefGoogle Scholar
  4. Boutry C, Matsumoto H, Airinei G, Benamouzig R, Tomé D, Blachier F, Bos C (2011) Monosodium glutamate raises antral distension and plasma amino acid after a standard meal in humans. Am J Physiol 300:G137–G145Google Scholar
  5. Brosnan JT, Brosnan ME (2012) Glutamate: a truly functional amino acid. Amino Acids. doi:10.1007/s00726-012-1280-4 PubMedGoogle Scholar
  6. Burrin DG, Stoll B (2009) Metabolic fate and function of dietary glutamate in the gut. Am J Clin Nutr 90:850S–856SPubMedCrossRefGoogle Scholar
  7. Chen LX, Yin YL, Jobgen WS, Jobgen SC, Knabe DA, Hu WX, Wu G (2007) In vitro oxidation of essential amino acids by intestinal mucosal cells of growing pigs. Livest Sci 109:19–23CrossRefGoogle Scholar
  8. Chen LX, Li P, Wang JJ, Li XL, Gao HJ, Yin YL, Hou YQ, Wu G (2009) Catabolism of nutritionally essential amino acids in developing porcine enterocytes. Amino Acids 37:143–152PubMedCrossRefGoogle Scholar
  9. Curi R, Lagranha CJ, Doi SQ, Sellitti DF, Procopio J, Pithon-Curi TC, Corless M, Newsholme P (2005) Molecular mechanisms of glutamine action. J Cell Physiol 204:392–401PubMedCrossRefGoogle Scholar
  10. Curthoys NP, Watford M (1995) Regulation of glutaminase activity and glutamine metabolism. Annu Rev Nutr 15:133–159PubMedCrossRefGoogle Scholar
  11. Dai ZL, Zhang J, Wu G, Zhu WY (2010) Utilization of amino acids by bacteria from the pig small intestine. Amino Acids 39:1201–1215PubMedCrossRefGoogle Scholar
  12. Dai ZL, Wu G, Zhu WY (2011) Amino acid metabolism in intestinal bacteria: links between gut ecology and host health. Front Biosci 16:1768–1786PubMedCrossRefGoogle Scholar
  13. Dai ZL, Li XL, Xi PB, Zhang J, Wu G, Zhu WY (2012a) Metabolism of select amino acids in bacteria from the pig small intestine. Amino Acids 42:1597–1608PubMedCrossRefGoogle Scholar
  14. Dai ZL, Li XL, Xi PB, Zhang J, Wu G, Zhu WY (2012b) Regulatory role for l-arginine in the utilization of amino acids by pig small-intestinal bacteria. Amino Acids 43:233–244PubMedCrossRefGoogle Scholar
  15. Dai ZL, Li XL, Xi PB, Zhang J, Wu G, Zhu WY (2012c) l-Glutamine regulates amino acid utilization by intestinal bacteria. Amino Acids. doi:10.1007/s00726-012-1264-4 Google Scholar
  16. Dugan MER, Knabe DA, Wu G (1994) Glutamine and glucose metabolism in intraepithelial lymphocytes from pre and post-weaning pigs. Comp Biochem Physiol 109B:675–681Google Scholar
  17. Flynn NE, Wu G (1996) An important role for endogenous synthesis of arginine in maintaining arginine homeostasis in neonatal pigs. Am J Physiol 271:R1149–R1155PubMedGoogle Scholar
  18. Haynes TE, Li P, Li XL, Shimotori K, Sato H, Flynn NE, Wang JJ, Knabe DA, Wu G (2009) L-Glutamine or L-alanyl-L-glutamine prevents oxidant- or endotoxin-induced death of neonatal enterocytes. Amino Acids 37:131–142Google Scholar
  19. Horvath K, Jami M, Hill ID, Papadimitriou JC, Magder LS, Chanasongcram S (1994) Isocaloric glutamine-free diet and the morphology and function of rat small intestine. J Parent Enter Nutr 20:128–134CrossRefGoogle Scholar
  20. Iwatsuki K, Torii K (2012) Peripheral chemosensing system for tastants and nutrients. Curr Opin Endocrinol Diabetes Obes 19:19–25PubMedGoogle Scholar
  21. Janeczko M, Stoll B, Chang X, Guan X, Burrin DG (2007) Extensive gut metabolism limits the intestinal absorption of excessive supplemental dietary glutamate loads in infant pigs. J Nutr 137:1284–1390Google Scholar
  22. Jobgen WJ, Meininger CJ, Jobgen SC, Li P, Lee MJ, Smith SB, Spencer TE, Fried SK, Wu G (2009) Dietary l-arginine supplementation reduces white-fat gain and enhances skeletal muscle and brown fat masses in diet-induced obese rats. J Nutr 139:230–237PubMedGoogle Scholar
  23. Kim SW, Wu G (2004) Dietary arginine supplementation enhances the growth of milk-fed young pigs. J Nutr 134:625–630PubMedGoogle Scholar
  24. Kirchgessner AL (2001) Glutamate in the enteric nervous system. Curr Opin Pharmacol 1:591–596PubMedCrossRefGoogle Scholar
  25. Klein RM, McKenzie JC (1983) The role of cell renewal in the ontogeny of the intestine. I. Cell proliferation patterns in adult, fetal, and neonatal intestine. J Pediatr Gastroenterol Nutr 2:10–43PubMedCrossRefGoogle Scholar
  26. Kondoh T, Torii K (2008) MSG intake suppresses weight gain, fat deposition, and plasma leptin levels in male Sprague-Dawley rats. Physiol Behav 95:135–144PubMedCrossRefGoogle Scholar
  27. Lalles JP, Bosi P, Smidt H, Stokes CR (2007) Weaning—a challenge to gut physiologists. Livest Sci 108:82–93CrossRefGoogle Scholar
  28. Li P, Kim SW, Li XL, Datta S, Pond WG, Wu G (2009) Dietary supplementation with cholesterol and docosahexaenoic acid affects concentrations of amino acids in tissues of young pigs. Amino Acids 37:709–716PubMedCrossRefGoogle Scholar
  29. Li XL, Rezaei R, Li P, Wu G (2011) Composition of amino acids in feed ingredients for animal diets. Amino Acids 40:1159–1168PubMedCrossRefGoogle Scholar
  30. Liu T, Peng J, Xiong Y, Zhou S, Cheng X (2002) Effects of dietary glutamine and glutamate supplementation on small intestinal structure, active absorption and DNA, RNA concentrations in skeletal muscle tissue of weaned piglets during d 28 to 42 of age. Asian-Aust J Anim Sci 15:238–242Google Scholar
  31. Mahan DC, Wiseman TD, Weaver E, Russell L (1999) Effect of supplemental sodium chloride and hydrochloric acid added to initial starter diets containing spray-dried blood plasma and lactose on resulting performance and nitrogen digestibility of 3-week-old weaned pigs. J Anim Sci 77:3016–3021PubMedGoogle Scholar
  32. Mateo RD, Wu G, Moon HK, Carroll JA, Kim SW (2008) Effects of dietary arginine supplementation during gestation and lactation on the performance of lactating primiparous sows and nursing piglets. J Anim Sci 86:827–835PubMedCrossRefGoogle Scholar
  33. Murphy JM, Murch SJ, Ball RO (1996) Proline is synthesized from glutamate during intragastric infusion but not during intravenous infusion in neonatal pigs. J Nutr 126:878–886PubMedGoogle Scholar
  34. Ou DY, Li DF, Cao YH, Li XL, Yin JD, Qiao SY, Wu G (2007) Dietary supplementation with zinc oxide decreases expression of the stem cell factor in the SI of weanling pigs. J Nutr Biochem 18:820–826PubMedCrossRefGoogle Scholar
  35. Plusk JR, Hampson DJ, Williams IH (1997) Factors influencing the structure and function of the small intestine in the weaned pig: a review. Livest Prod Sci 51:215–236CrossRefGoogle Scholar
  36. Prasad AS, DuMouchelle E, Koniuch D, Oberleas D (1972) A simple fluorometric method for the determination of RNA and DNA in tissues. J Lab Clin Med 80:598–601PubMedGoogle Scholar
  37. Rhoads JM, Wu G (2009) Glutamine, arginine, and leucine signaling in the intestine. Amino Acids 37:111–122CrossRefGoogle Scholar
  38. San Gabriel A, Uneyama H (2012) Amino acid sensing in the gastrointestinal tract. Amino Acids. doi:10.1007/s00726-012-1371-2 PubMedGoogle Scholar
  39. San Gabriel A, Nakamura E, Uneyama H, Torii K (2009) Taste, visceral information and exocrine reflexes with glutamate through umami receptors. J Med Invest 56(Suppl):209–217PubMedCrossRefGoogle Scholar
  40. Satterfield MC, Dunlap KA, Keisler DH, Bazer FW, Wu G (2012) Arginine nutrition and fetal brown adipose tissue development in nutrient-restricted sheep. Amino Acids. doi:10.1007/s00726-011-1168-8 Google Scholar
  41. Smriga M, Torii K (2000) Release of hypothalamic norepinephrine during MSG intake in rats fed normal and nonprotein diet. Physiol Behav 70:413–415PubMedCrossRefGoogle Scholar
  42. Smriga M, Murakami H, Mori M, Torii K (2000) Use of thermal photography to explore the age-dependent effect of monosodium glutamate, NaCl and glucose on brown adipose tissue thermogenesis. Physiol Behav 71:403–407PubMedCrossRefGoogle Scholar
  43. Somekawa S, Hayashi N, Niijima A, Uneyama H, Torii K (2012) Dietary free glutamate prevents diarrhoea during intra-gastric tube feeding in a rat model. Br J Nutr 107:20–23PubMedCrossRefGoogle Scholar
  44. Stoll B, Burrin DG, Henry J, Yu H, Jahoor F, Reeds PJ (1999) Substrate oxidation by the portal drained viscera of fed pigs. Am J Physiol 277:E168–E175PubMedGoogle Scholar
  45. Strous GJ, Dekker J (1992) Mucin-type glycoproteins. Crit Rev Biochem Mol Biol 27:57–92PubMedCrossRefGoogle Scholar
  46. Wang JJ, Chen LX, Li P, Li XL, Zhou HJ, Wang FL, Li DF, Yin YL, Wu G (2008) Gene expression is altered in piglet small intestine by weaning and dietary glutamine supplementation. J Nutr 138:1025–1032PubMedGoogle Scholar
  47. Wei JW, Carroll RJ, Harden KK, Wu G (2012) Comparisons of treatment means when factors do not interact in two-factorial studies. Amino Acids 42:2031–2035PubMedCrossRefGoogle Scholar
  48. Wu G (1998) Intestinal mucosal amino acid catabolism. J Nutr 128:1249–1252PubMedGoogle Scholar
  49. Wu G (2009) Amino acids: metabolism, functions, and nutrition. Amino Acids 37:1–17PubMedCrossRefGoogle Scholar
  50. Wu G (2010) Functional amino acids in growth, reproduction and health. Adv Nutr 1:31–37PubMedCrossRefGoogle Scholar
  51. Wu G, Knabe DA (1995) Arginine synthesis in enterocytes of neonatal pigs. Am J Physiol 269:R621–R629PubMedGoogle Scholar
  52. Wu G, Morris SM (1998) Arginine metabolism: nitric oxide and beyond. Biochem J 336:1–17PubMedGoogle Scholar
  53. Wu G, Borbolla AG, Knabe DA (1994) The uptake of glutamine and release of arginine, citrulline and proline by the small intestine of developing pigs. J Nutr 124:2437–2444PubMedGoogle Scholar
  54. Wu G, Knabe DA, Yan W, Flynn NE (1995) Glutamine and glucose metabolism in enterocytes of the neonatal pig. Am J Physiol 268:R334–R342PubMedGoogle Scholar
  55. Wu G, Meier SA, Knabe DA (1996) Dietary Glutamine supplementation prevents jejunal atrophy in weaned pigs. J Nutr 126:2578–2584PubMedGoogle Scholar
  56. Wu G, Davis PK, Flynn NE, Knabe DA, Davidson JT (1997) Endogenous synthesis of arginine plays an important role in maintaining arginine homeostasis in postweaning growing pigs. J Nutr 127:2342–2349Google Scholar
  57. Wu G, Fang YZ, Yang S, Lupton JR, Turner ND (2004) Glutathione metabolism and its implications for health. J Nutr 134:489–492PubMedGoogle Scholar
  58. Wu G, Bazer FW, Johnson GA, Knabe DA, Burghardt RC, Spencer TE, Li XL, Wang JJ (2011) Important roles for l-glutamine in swine nutrition and production. J Anim Sci 89:2017–2030PubMedCrossRefGoogle Scholar
  59. Yao K, Yin YL, Li XL, Xi PB, Wang JJ, Lei J, Hou YQ, Wu G (2012) Alpha-ketoglutarate inhibits glutamine degradation and enhances protein synthesis in intestinal porcine epithelial cells. Amino Acids 42:2491–2500PubMedCrossRefGoogle Scholar
  60. Zimmerman DR (1975) Glutamic acid and tryptophan additions to a low-protein pig starter. J Anim Sci 40:871–874PubMedGoogle Scholar

Copyright information

© Springer-Verlag Wien 2012

Authors and Affiliations

  • Reza Rezaei
    • 1
    • 2
  • Darrell A. Knabe
    • 1
  • Carmen D. Tekwe
    • 2
    • 3
  • Sudath Dahanayaka
    • 1
  • Martin D. Ficken
    • 4
  • Susan E. Fielder
    • 4
  • Sarah J. Eide
    • 4
  • Sandra L. Lovering
    • 4
  • Guoyao Wu
    • 1
    • 2
  1. 1.Department of Animal ScienceTexas A&M UniversityCollege StationUSA
  2. 2.Faculty of NutritionTexas A&M UniversityCollege StationUSA
  3. 3.Department of StatisticsTexas A&M UniversityCollege StationUSA
  4. 4.Texas A&M Veterinary Medical Diagnostic LaboratoryCollege StationUSA

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