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Amino Acids

, Volume 43, Issue 2, pp 717–724 | Cite as

l-Leucine induces growth arrest and persistent ERK activation in glioma cells

  • Satoru TakeuchiEmail author
  • Hiroshi NawashiroEmail author
  • Kojiro Wada
  • Namiko Nomura
  • Terushige Toyooka
  • Naoki Otani
  • Hideo Osada
  • Hirotaka Matsuo
  • Nariyoshi Shinomiya
Original Article
  • 228 Downloads

Abstract

Glioma is the most common type of brain tumor, and has the worst prognosis in human malignancy. Experimental evidence suggests that the use of high concentrations of various amino acids may perturb neoplastic cell growth. Thus, the aim of this study was to investigate whether essential amino acids can alter the growth and proliferation of glioma cells. Studies were performed using C6 rat glioma cell lines. High concentration of l-leucine induced growth arrest of glioma cell lines. Terminal transferase uridyl nick end labeling assay and cell cycle analysis showed that the effect of l-leucine on glioma cells growth was not cytotoxic, but rather cytostatic. Additionally, the extracellular signal-regulated protein kinase was activated in l-leucine-treated glioma cells, and inhibition of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase 1 (MEK) enhanced the effect of l-leucine on glioma cell growth. These data suggest that high concentration l-leucine combined with inhibition of MEK is a potential strategy for glioma cell growth arrest.

Keywords

Essential amino acid l-Leucine Glioma Extracellular signal-regulated protein kinase L-type amino acid transporter 1 

Notes

Acknowledgments

We thank Akiko Yano for technical assistance in preparation of data for the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Satoru Takeuchi
    • 1
    Email author
  • Hiroshi Nawashiro
    • 1
    Email author
  • Kojiro Wada
    • 1
  • Namiko Nomura
    • 1
  • Terushige Toyooka
    • 1
  • Naoki Otani
    • 1
  • Hideo Osada
    • 1
  • Hirotaka Matsuo
    • 2
  • Nariyoshi Shinomiya
    • 2
  1. 1.Department of NeurosurgeryNational Defense Medical CollegeTokorozawaJapan
  2. 2.Department of Integrative Physiology and Bio-Nano MedicineNational Defense Medical CollegeTokorozawaJapan

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