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Amino Acids

, Volume 43, Issue 1, pp 327–335 | Cite as

Different environmental temperatures affect amino acid metabolism in the eurytherm teleost Senegalese sole (Solea senegalensis Kaup, 1858) as indicated by changes in plasma metabolites

  • Benjamín Costas
  • Cláudia Aragão
  • Ignacio Ruiz-Jarabo
  • Luis Vargas-Chacoff
  • Francisco J. Arjona
  • Juan M. Mancera
  • Maria T. Dinis
  • Luís E. C. Conceição
Original Article

Abstract

Senegalese sole (Solea senegalensis) is a eurytherm teleost that under natural conditions can be exposed to annual water temperature fluctuations between 12 and 26°C. This study assessed the effects of temperature on sole metabolic status, in particular in what concerns plasma free amino acid changes during thermal acclimation. Senegalese sole maintained at 18°C were acclimated to either cold (12°C) or warm (26°C) environmental temperatures for 21 days. Fish maintained at 18°C served as control. Plasma concentrations of cortisol, glucose, lactate, triglycerides, proteins, and free amino acids were assessed. Cold acclimation influenced interrenal responses of sole by increasing cortisol release. Moreover, plasma glucose and lactate concentrations increased linearly with temperature, presumably reflecting a higher metabolic activity of sole acclimated to 26°C. Acclimation temperature affected more drastically plasma concentrations of dispensable than that of indispensable amino acids, and different acclimation temperatures induced different responses. Asparagine, glutamine and ornithine seem to be of particular importance for ammonia detoxification mechanisms, synthesis of triglycerides that may be used during homeoviscous adaptation and, to a lesser extent, as energetic substrates in specimens acclimated to 12°C. When sole is acclimated to 26°C taurine, glutamate, GABA and glycine increased, which may suggest important roles as antioxidant defences, in osmoregulatory processes and/or for energetic purposes at this thermal regimen. In conclusion, acclimation to different environmental temperatures induces several metabolic changes in Senegalese sole, suggesting that amino acids may be important for thermal acclimation.

Keywords

Acclimation Dispensable amino acids Homeoviscous adaptation Solea senegalensis Temperature 

Notes

Acknowledgments

This study was supported by projects STRESSAA–POCTI/CVT/49324/2002 (FCT, Portugal and FEDER), AGL2007-61211/ACU (Ministerio de Educación y Ciencia, Spain) and Proyecto de Excelencia PO7-RNM-02843 (Consejería de Innovación, Ciencia y Empresa. Junta de Andalucía) to J.M.M. Benjamín Costas and Cláudia Aragão were supported by Fundação para a Ciência e Tecnologia, Portugal (SFRH/BD/38697/2007 and SFRH/BPD/37197/2007, respectively). The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Benjamín Costas
    • 1
    • 4
  • Cláudia Aragão
    • 1
  • Ignacio Ruiz-Jarabo
    • 2
  • Luis Vargas-Chacoff
    • 2
    • 3
  • Francisco J. Arjona
    • 2
  • Juan M. Mancera
    • 2
  • Maria T. Dinis
    • 1
  • Luís E. C. Conceição
    • 1
  1. 1.CCMAR-CIMAR L.A., Centro de Ciências do Mar do AlgarveUniversidade do Algarve, Campus de GambelasFaroPortugal
  2. 2.Departamento de Biología, Facultad de Ciencias del Mar y AmbientalesUniversidad de CádizCádizEspaña
  3. 3.Instituto de Ciencias Marinas y Limnológicas, Facultad de CienciasUniversidad Austral de ChileValdiviaChile
  4. 4.CIIMAR-CIMAR L.A., Centro Interdisciplinar de Investigação Marinha e AmbientalPortoPortugal

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