Amino Acids

, Volume 42, Issue 6, pp 2461–2472

Effect of two β-alanine dosing protocols on muscle carnosine synthesis and washout

  • Trent Stellingwerff
  • Helen Anwander
  • Andrea Egger
  • Tania Buehler
  • Roland Kreis
  • Jacques Decombaz
  • Chris Boesch
Original Article

DOI: 10.1007/s00726-011-1054-4

Cite this article as:
Stellingwerff, T., Anwander, H., Egger, A. et al. Amino Acids (2012) 42: 2461. doi:10.1007/s00726-011-1054-4

Abstract

Carnosine (β-alanyl-l-histidine) is found in high concentrations in skeletal muscle and chronic β-alanine (BA) supplementation can increase carnosine content. This placebo-controlled, double-blind study compared two different 8-week BA dosing regimens on the time course of muscle carnosine loading and 8-week washout, leading to a BA dose–response study with serial muscle carnosine assessments throughout. Thirty-one young males were randomized into three BA dosing groups: (1) high–low: 3.2 g BA/day for 4 weeks, followed by 1.6 g BA/day for 4 weeks; (2) low–low: 1.6 g BA/day for 8 weeks; and (3) placebo. Muscle carnosine in tibialis-anterior (TA) and gastrocnemius (GA) muscles was measured by 1H-MRS at weeks 0, 2, 4, 8, 12 and 16. Flushing symptoms and blood clinical chemistry were trivial in all three groups and there were no muscle carnosine changes in the placebo group. During the first 4 weeks, the increase for high–low (TA 2.04 mmol/kgww, GA 1.75 mmol/kgww) was ~twofold greater than low–low (TA 1.12 mmol/kgww, GA 0.80 mmol/kgww). 1.6 g BA/day significantly increased muscle carnosine within 2 weeks and induced continual rises in already augmented muscle carnosine stores (week 4–8, high–low regime). The dose–response showed a carnosine increase of 2.01 mmol/kgww per 100 g of consumed BA, which was only dependent upon the total accumulated BA consumed (within a daily intake range of 1.6–3.2 g BA/day). Washout rates were gradual (0.18 mmol/kgww and 0.43 mmol/kgww/week; ~2%/week). In summary, the absolute increase in muscle carnosine is only dependent upon the total BA consumed and is not dependent upon baseline muscle carnosine, the muscle type, or the daily amount of supplemented BA.

Keywords

β-alanine Carnosine Muscle Synthesis Washout Dose–response 

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Trent Stellingwerff
    • 1
  • Helen Anwander
    • 2
  • Andrea Egger
    • 2
  • Tania Buehler
    • 2
  • Roland Kreis
    • 2
  • Jacques Decombaz
    • 1
  • Chris Boesch
    • 2
  1. 1.Department of Nutrition and HealthNestlé Research Center, Nestec LtdLausanne 26Switzerland
  2. 2.University of BernBernSwitzerland

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