Amino Acids

, Volume 42, Issue 6, pp 2461–2472

Effect of two β-alanine dosing protocols on muscle carnosine synthesis and washout

  • Trent Stellingwerff
  • Helen Anwander
  • Andrea Egger
  • Tania Buehler
  • Roland Kreis
  • Jacques Decombaz
  • Chris Boesch
Original Article

Abstract

Carnosine (β-alanyl-l-histidine) is found in high concentrations in skeletal muscle and chronic β-alanine (BA) supplementation can increase carnosine content. This placebo-controlled, double-blind study compared two different 8-week BA dosing regimens on the time course of muscle carnosine loading and 8-week washout, leading to a BA dose–response study with serial muscle carnosine assessments throughout. Thirty-one young males were randomized into three BA dosing groups: (1) high–low: 3.2 g BA/day for 4 weeks, followed by 1.6 g BA/day for 4 weeks; (2) low–low: 1.6 g BA/day for 8 weeks; and (3) placebo. Muscle carnosine in tibialis-anterior (TA) and gastrocnemius (GA) muscles was measured by 1H-MRS at weeks 0, 2, 4, 8, 12 and 16. Flushing symptoms and blood clinical chemistry were trivial in all three groups and there were no muscle carnosine changes in the placebo group. During the first 4 weeks, the increase for high–low (TA 2.04 mmol/kgww, GA 1.75 mmol/kgww) was ~twofold greater than low–low (TA 1.12 mmol/kgww, GA 0.80 mmol/kgww). 1.6 g BA/day significantly increased muscle carnosine within 2 weeks and induced continual rises in already augmented muscle carnosine stores (week 4–8, high–low regime). The dose–response showed a carnosine increase of 2.01 mmol/kgww per 100 g of consumed BA, which was only dependent upon the total accumulated BA consumed (within a daily intake range of 1.6–3.2 g BA/day). Washout rates were gradual (0.18 mmol/kgww and 0.43 mmol/kgww/week; ~2%/week). In summary, the absolute increase in muscle carnosine is only dependent upon the total BA consumed and is not dependent upon baseline muscle carnosine, the muscle type, or the daily amount of supplemented BA.

Keywords

β-alanine Carnosine Muscle Synthesis Washout Dose–response 

References

  1. Abe H (2000) Role of histidine-related compounds as intracellular proton buffering constituents in vertebrate muscle. Biochemistry (Mosc) 65:757–765Google Scholar
  2. Albani C, Blaser G, Geyer M, Schmutzer G, Brähler E, Bailer H et al (2005) Überprüfung der Gütekriterien der deutschen Kurzform des Fragebogens “Profile of Mood States” (POMS) in einer repräsentativen Bevolkerungsstichprobe. Psychother Psychiatr Med 55:324–330CrossRefGoogle Scholar
  3. Artioli GG, Gualano B, Smith A, Stout J, Lancha AH Jr (2010) Role of beta-alanine supplementation on muscle carnosine and exercise performance. Med Sci Sports Exerc 42:1162–1173PubMedGoogle Scholar
  4. Asatoor AM, Bandoh JK, Lant AF, Milne MD, Navab F (1970) Intestinal absorption of carnosine and its constituent amino acids in man. Gut 11:250–254PubMedCrossRefGoogle Scholar
  5. Baguet A, Reyngoudt H, Pottier A, Everaert I, Callens S, Achten E et al (2009) Carnosine loading and washout in human skeletal muscles. J Appl Physiol 106:837–842PubMedCrossRefGoogle Scholar
  6. Baguet A, Bourgois J, Vanhee L, Achten E, Derave W (2010a) Important role of muscle carnosine in rowing performance. J Appl Physiol 109:1096–1101PubMedCrossRefGoogle Scholar
  7. Baguet A, Koppo K, Pottier A, Derave W (2010b) Beta-alanine supplementation reduces acidosis but not oxygen uptake response during high-intensity cycling exercise. Eur J Appl Physiol 108:495–503PubMedCrossRefGoogle Scholar
  8. Bakardjiev A, Bauer K (1994) Transport of beta-alanine and biosynthesis of carnosine by skeletal muscle cells in primary culture. Eur J Biochem 225:617–623PubMedCrossRefGoogle Scholar
  9. Bate-Smith EC (1938) The buffering of muscle in rigor: protein, phosphate and carnosine. J Physiol 92:336–343Google Scholar
  10. Batrukova MA, Rubtsov AM (1997) Histidine-containing dipeptides as endogenous regulators of the activity of sarcoplasmic reticulum Ca-release channels. Biochim Biophys Acta 1324:142–150PubMedCrossRefGoogle Scholar
  11. Boesch C, Kreis R (2001) Dipolar coupling and ordering effects observed in magnetic resonance spectra of skeletal muscle. NMR Biomed 14:140–148PubMedCrossRefGoogle Scholar
  12. Boldyrev AA, Koldobski A, Kurella E, Maltseva V, Stvolinski S (1993) Natural histidine-containing dipeptide carnosine as a potent hydrophilic antioxidant with membrane stabilizing function. A biomedical aspect. Mol Chem Neuropathol 19:185–192PubMedCrossRefGoogle Scholar
  13. Decombaz J, Beaumont M, Vuichoud J, Bouisset F, Enslen M, Stellingwerff T (2011) The effect of slow-release β-alanine on absorption kinetics and paresthesia (abstract). Med Sci Sports Exerc 43:S2224CrossRefGoogle Scholar
  14. Derave W, Ozdemir MS, Harris RC, Pottier A, Reyngoudt H, Koppo K et al (2007) β-Alanine supplementation augments muscle carnosine content and attenuates fatigue during repeated isokinetic contraction bouts in trained sprinters. J Appl Physiol 103:1736–1743PubMedCrossRefGoogle Scholar
  15. Derave W, Everaert I, Beeckman S, Baguet A (2010) Muscle carnosine metabolism and beta-alanine supplementation in relation to exercise and training. Sports Med 40:247–263PubMedCrossRefGoogle Scholar
  16. Dunnett M, Harris RC (1995) Carnosine and taurine contents of different fibre types in the middle gluteal muscle of the thoroughbred horse. Equine Vet J S18:214–217Google Scholar
  17. Everaert I, Mooyaart A, Baguet A, Zutinic A, Baelde H, Achten E et al (2011) Vegetarianism, female gender and increasing age, but not CNDP1 genotype, are associated with reduced muscle carnosine levels in humans. Amino Acids 40:1221–1229PubMedCrossRefGoogle Scholar
  18. Flancbaum L, Fitzpatrick JC, Brotman DN, Marcoux AM, Kasziba E, Fisher H (1990) The presence and significance of carnosine in histamine-containing tissues of several mammalian species. Agents Actions 31:190–196PubMedCrossRefGoogle Scholar
  19. Harris RC, Soderlund K, Hultman E (1992) Elevation of creatine in resting and exercised muscle of normal subjects by creatine supplementation. Clin Sci 8:367–374Google Scholar
  20. Harris RC, Tallon MJ, Dunnett M, Boobis L, Coakley J, Kim HJ et al (2006) The absorption of orally supplied beta-alanine and its effect on muscle carnosine synthesis in human vastus lateralis. Amino Acids 30:279–289PubMedCrossRefGoogle Scholar
  21. Harris RC, Jones GA, Wise JA (2008) The plasma concentration-time profile of beta-alanine using a controlled-release formulation (Carnosyn) (abstract). FASEB J 22:701.9Google Scholar
  22. Hill CA, Harris RC, Kim HJ, Harris BD, Sale C, Boobis LH et al (2007) Influence of beta-alanine supplementation on skeletal muscle carnosine concentrations and high intensity cycling capacity. Amino Acids 32:225–233PubMedCrossRefGoogle Scholar
  23. Hipkiss AR (2005) Glycation, ageing and carnosine: are carnivorous diets beneficial? Mech Ageing Dev 126:1034–1039PubMedCrossRefGoogle Scholar
  24. Hoffman J, Ratamess N, Kang J, Mangine G, Faigenbaum A, Stout J (2006) Effect of creatine and beta-alanine supplementation on performance and endocrine responses in strength/power athletes. Int J Sport Nutr Exerc Metab 16:430–446PubMedGoogle Scholar
  25. Hultman E, Soderlund K, Timmons JA, Cederblad G, Greenhaff PL (1996) Muscle creatine loading in men. J Appl Physiol 81:232–237PubMedGoogle Scholar
  26. Kendrick IP, Harris RC, Kim HJ, Kim CK, Dang VH, Lam TQ et al (2008) The effects of 10 weeks of resistance training combined with beta-alanine supplementation on whole body strength, force production, muscular endurance and body composition. Amino Acids 34:547–554PubMedCrossRefGoogle Scholar
  27. Kendrick IP, Kim HJ, Harris RC, Kim CK, Dang VH, Lamb TQ et al (2009) The effect of 4 weeks beta-alanine supplementation and isokinetic training on carnosine concentrations in type I and II human skeletal muscle fibres. Eur J Appl Physiol 106:131–138PubMedCrossRefGoogle Scholar
  28. Kley RA, Tarnopolsky MA, Vorgerd M (2011) Creatine for treating muscle disorders. Cochrane Database Syst Rev 2:CD004760Google Scholar
  29. Naressi A, Couturier C, Devos JM, Janssen M, Mangeat C, de Beer R et al (2001) Java-based graphical user interface for the MRUI quantitation package. Magma 12:141–152PubMedCrossRefGoogle Scholar
  30. Ozdemir MS, Reyngoudt H, De Deene Y, Sazak HS, Fieremans E, Delputte S et al (2007) Absolute quantification of carnosine in human calf muscle by proton magnetic resonance spectroscopy. Phys Med Biol 52:6781–6794PubMedCrossRefGoogle Scholar
  31. Park YJ, Volpe SL, Decker EA (2005) Quantitation of carnosine in humans plasma after dietary consumption of beef. J Agric Food Chem 53:4736–4739PubMedCrossRefGoogle Scholar
  32. Parkhouse WS, McKenzie DC (1984) Possible contribution of skeletal muscle buffers to enhanced anaerobic performance: a brief review. Med Sci Sports Exerc 16:328–338PubMedGoogle Scholar
  33. Sale C, Saunders B, Harris RC (2010) Effect of beta-alanine supplementation on muscle carnosine concentrations and exercise performance. Amino Acids 39:321–333PubMedCrossRefGoogle Scholar
  34. Sale C, Saunders B, Hudson S, Wise JA, Harris RC, Sunderland CD (2011) Effect of beta-alanine plus sodium bicarbonate on high-intensity cycling capacity. Med Sci Sports Exerc. doi:10.1249/MSS.0b013e3182188501
  35. Schroder L, Bachert P (2003) Evidence for a dipolar-coupled AM system in carnosine in human calf muscle from in vivo 1H NMR spectroscopy. J Magn Reson 164:256–269PubMedCrossRefGoogle Scholar
  36. Shacham S (1983) A shortened version of the profile of mood states. J Pers Assess 47:305–306PubMedCrossRefGoogle Scholar
  37. Shen Y, Zhang S, Fu L, Hu W, Chen Z (2008) Carnosine attenuates mast cell degranulation and histamine release induced by oxygen-glucose deprivation. Cell Biochem Funct 26:334–338PubMedCrossRefGoogle Scholar
  38. Spielberger CD, Gorsuch RL, Lushene PR, Vagg PR, Jacobs AG (1983) Manual for the state-trait anxiety inventory (Form Y). Consulting Psychologists Press Inc., Palo Alto, CA, p 36Google Scholar
  39. Stout JR, Cramer JT, Mielke M, O’Kroy J, Torok DJ, Zoeller RF (2006) Effects of twenty-eight days of beta-alanine and creatine monohydrate supplementation on the physical working capacity at neuromuscular fatigue threshold. J Strength Cond Res 20:928–931PubMedGoogle Scholar
  40. Zoeller RF, Stout JR, O’Kroy JA, Torok DJ, Mielke M (2007) Effects of 28 days of beta-alanine and creatine monohydrate supplementation on aerobic power, ventilatory and lactate thresholds, and time to exhaustion. Amino Acids 33:505–510PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Trent Stellingwerff
    • 1
  • Helen Anwander
    • 2
  • Andrea Egger
    • 2
  • Tania Buehler
    • 2
  • Roland Kreis
    • 2
  • Jacques Decombaz
    • 1
  • Chris Boesch
    • 2
  1. 1.Department of Nutrition and HealthNestlé Research Center, Nestec LtdLausanne 26Switzerland
  2. 2.University of BernBernSwitzerland

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