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Amino Acids

, Volume 42, Issue 6, pp 2513–2519 | Cite as

Epigallocatechin-3-O-gallate inhibits the production of thymic stromal lymphopoietin by the blockade of caspase-1/NF-κB pathway in mast cells

  • Phil-Dong Moon
  • In-Hwa Choi
  • Hyung-Min Kim
Short Communication

Abstract

The cytokine thymic stromal lymphopoietin (TSLP) has been implicated in the development and progression of allergic diseases such as atopic dermatitis, asthma, and chronic obstructive pulmonary disease. However, it has not yet been clarified the effect of epigallocatechin-3-O-gallate (EGCG) on the production of TSLP. Thus, we investigated how EGCG inhibits the production of TSLP in the human mast cell line (HMC-1) cells. Enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, luciferase assay, and Western blot analysis were used to investigate the effects of EGCG. EGCG inhibited the production and mRNA expression of TSLP in HMC-1 cells. EGCG also inhibited the nuclear factor-κB luciferase activity induced by phorbol myristate acetate plus A23187. Furthermore, EGCG inhibited the activation of caspase-1 in HMC-1 cells. These results provide evidence that EGCG can help us to treat inflammatory and atopic diseases through the inhibition of TSLP.

Keywords

Thymic stromal lymphopoietin Epigallocatechin-3-O-gallate Nuclear factor-κB Caspase-1 

Abbreviations

AD

Atopic dermatitis

EGCG

Epigallocatechin-3-O-gallate

NF-κB

Nuclear factor-κB

PMA

Phorbol myristate acetate

TSLP

Thymic stromal lymphopoietin

Notes

Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0005591) and by a grant from the Kyung Hee University in 2011 (KHU-20110092).

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Department of Pharmacology, College of Oriental MedicineInstitute of Oriental Medicine, Kyung Hee UniversitySeoulRepublic of Korea
  2. 2.Department of Oriental Dermatology, College of Oriental MedicineKyung Hee UniversitySeoulRepublic of Korea

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