Epigallocatechin-3-O-gallate inhibits the production of thymic stromal lymphopoietin by the blockade of caspase-1/NF-κB pathway in mast cells
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The cytokine thymic stromal lymphopoietin (TSLP) has been implicated in the development and progression of allergic diseases such as atopic dermatitis, asthma, and chronic obstructive pulmonary disease. However, it has not yet been clarified the effect of epigallocatechin-3-O-gallate (EGCG) on the production of TSLP. Thus, we investigated how EGCG inhibits the production of TSLP in the human mast cell line (HMC-1) cells. Enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, luciferase assay, and Western blot analysis were used to investigate the effects of EGCG. EGCG inhibited the production and mRNA expression of TSLP in HMC-1 cells. EGCG also inhibited the nuclear factor-κB luciferase activity induced by phorbol myristate acetate plus A23187. Furthermore, EGCG inhibited the activation of caspase-1 in HMC-1 cells. These results provide evidence that EGCG can help us to treat inflammatory and atopic diseases through the inhibition of TSLP.
KeywordsThymic stromal lymphopoietin Epigallocatechin-3-O-gallate Nuclear factor-κB Caspase-1
Phorbol myristate acetate
Thymic stromal lymphopoietin
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0005591) and by a grant from the Kyung Hee University in 2011 (KHU-20110092).
Conflict of interest
The authors declare that they have no conflict of interest.
- Boost KA, Hoegl S, Hofstetter C, Flondor M, Stegewerth K, Platacis I, Pfeilschifter J, Muhl H, Zwissler B (2007) Targeting caspase-1 by inhalation-therapy: effects of Ac-YVAD-CHO on IL-1 beta, IL-18 and downstream proinflammatory parameters as detected in rat endotoxaemia. Intensive Care Med 33:863–871PubMedCrossRefGoogle Scholar
- Dumortier A, Durham AD, Di Piazza M, Vauclair S, Koch U, Ferrand G, Ferrero I, Demehri S, Song LL, Farr AG, Leonard WJ, Kopan R, Miele L, Hohl D, Finke D, Radtke F (2010) Atopic dermatitis-like disease and associated lethal myeloproliferative disorder arise from loss of Notch signaling in the murine skin. PLoS One 5:e9258PubMedCrossRefGoogle Scholar
- Friend SL, Hosier S, Nelson A, Foxworthe D, Williams DE, Farr A (1994) A thymic stromal cell line supports in vitro development of surface IgM+ B cells and produces a novel growth factor affecting B and T lineage cells. Exp Hematol 22(3):321–328Google Scholar
- Kang JS, Yoon WK, Youm JK, Jeong SK, Park BD, Han MH, Lee H, Moon EY, Han SB, Lee CW, Lee K, Park SK, Yang KH, Kim HM (2008) Inhibition of atopic dermatitis-like skin lesions by topical application of a novel ceramide derivative, K6PC-9p, in NC/Nga mice. Exp Dermatol 17:958–964CrossRefGoogle Scholar
- Ozawa T, Koyama K, Ando T, Ohnuma Y, Hatsushika K, Ohba T, Sugiyama H, Hamada Y, Ogawa H, Okumura K, Nakao A (2007) Thymic stromal lymphopoietin secretion of synovial fibroblasts is positively and negatively regulated by Toll-like receptors/nuclear factor-kappaB pathway and interferon-gamma/dexamethasone. Mod Rheumatol 17:459–463PubMedCrossRefGoogle Scholar
- Reefer AJ, Hulse KE, Lannigan JA, Solga MD, Wright PW, Kelly LA, Patrie J, Chapman MD, Woodfolk JA (2010) Flow cytometry imaging identifies rare T(H)2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu. J Allergy Clin Immunol 126:105–1049 1058.e1–e10CrossRefGoogle Scholar
- Schoonbroodt S, Legrand-Poels S, Best-Belpomme M, Piette J (1997) Activation of the NF-kappaB transcription factor in a T-lymphocytic cell line by hypochlorous acid. Biochem J 321:777–785Google Scholar
- Zheng Y, Morris A, Sunkara M, Layne J, Toborek M, Hennig B (2011) Epigallocatechin-gallate stimulates NF-E2-related factor and heme oxygenase-1 via caveolin-1 displacement. J Nutr Biochem (Epub ahead of print)Google Scholar