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Amino Acids

, Volume 42, Issue 6, pp 2439–2445 | Cite as

The reaction of methionine with hydroxyl radical: reactive intermediates and methanethiol production

  • Ivan SpasojevićEmail author
  • Jelena Bogdanović Pristov
  • Ljubodrag Vujisić
  • Mihajlo Spasić
Original Article

Abstract

The mechanisms of reaction of methionine with hydroxyl radical are not fully understood. Here, we unequivocally show using electron paramagnetic resonance spin-trapping spectroscopy and GC–FID and GC–MS, the presence of specific carbon-, nitrogen- and sulfur-centered radicals as intermediates of this reaction, as well as the liberation of methanethiol as a gaseous end product. Taking into account the many roles that methionine has in eco- and biosystems, our results may elucidate redox chemistry of this amino acid and processes that methionine is involved in.

Keywords

Methionine Hydroxyl radical EPR GC Free radical Methanethiol 

Notes

Acknowledgments

This work was supported by the Ministry of Science of the Republic of Serbia, Grant numbers 173014 and 173017. We are thankful to Mladen Simonović at the Institute of General and Physical Chemistry, University of Belgrade for technical help.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Ivan Spasojević
    • 1
    Email author
  • Jelena Bogdanović Pristov
    • 1
  • Ljubodrag Vujisić
    • 2
  • Mihajlo Spasić
    • 3
  1. 1.Institute for Multidisciplinary ResearchUniversity of BelgradeBelgradeSerbia
  2. 2.Faculty of ChemistryUniversity of BelgradeBelgradeSerbia
  3. 3.Institute for Biological ResearchUniversity of BelgradeBelgradeSerbia

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