Amino Acids

, Volume 42, Issue 6, pp 2149–2163 | Cite as

Identification of c-myc-dependent proteins in the medulloblastoma cell line D425Med

Original Article

Abstract

High c-myc levels are linked to poor prognosis in medulloblastoma (MB), and it was the aim of the current study to search for c-myc-dependent proteins in the MB cell line D425Med. For this purpose D425Med cells and cells with knocked-down c-myc (by siRNA) were analysed by a gel-based differential proteomics study using mass spectrometry. Heterogeneous nuclear ribonucleoproteins C1/C2, heterogeneous nuclear ribonucleoprotein A/B, stathmin, endoplasmic reticulum protein ERp29 precursor and guanidinoacetate N-methyltransferase were c-myc dependently expressed. Signalling, the protein machinery, metabolism and endoplasmic reticulum function may be affected and these results enable studying tumour tissue for these proteins as potential dignity markers or pharmacological targets.

Keywords

Brain tumour Medulloblastoma c-myc myc 

Abbreviations

2DE

Two-dimensional gel electrophoresis

GAMT

Guanidinoacetate N-methyltransferase

hnRNP

Heterogeneous nuclear ribonucleoprotein

hTERT

Human telomerase reverse transcriptase

MB

Medulloblastoma

PNET

Primitive neuroectodermal tumour

Supplementary material

726_2011_953_MOESM1_ESM.doc (160 kb)
Supplementary material 1 (DOC 160 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Department of PediatricsMedical University of ViennaViennaAustria
  2. 2.Institute of NeurologyMedical University of ViennaViennaAustria

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