Mcm1p binding sites in the ARG1 promoter positively regulate ARG1 transcription and S. cerevisiae growth in the absence of arginine and Gcn4p
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In this study, we investigated the activating role of Mcm1p at ARG1 during arginine starvation. Our results showed that two Mcm1p binding sites positively contribute to ARG1 transcription and cell growth. Especially, we provide strong evidence that the Mcm1p binding sites play a positive role in ARG1 transcription to overcome arginine starvation in the absence of Gcn4p. In addition, we found that the Mcm1p binding sites are not only regulated by the presence or absence of arginine but also in the presence or absence of other amino acids. These findings suggest that the ARG1 promoter utilizes different DNA elements to control transcription, depending on which amino acids are detected in the medium.
KeywordsGcn4p Mcm1p ARG1 SD and SC media Arginine starvation
Synthetic defined minimal
Synthetic defined complete
MCM1, AGAMOUS, DEFICIENS, and serum response factor
We thank Marjolaine Crabeel for the SS5 strain and M13mp7-ARG1. This work was supported by Basic Science Program through the National Research Foundation of Korea Grant funded by the Korean Government (NRF-2009-0070791).
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