Amino Acids

, Volume 40, Issue 1, pp 221–238 | Cite as

Characterization, using comparative proteomics, of differentially expressed proteins in the hippocampus of the mesial temporal lobe of epileptic rats following treatment with valproate

  • Liwen Wu
  • Jing Peng
  • Chaoping Wei
  • Gu Liu
  • Guoli Wang
  • Kongzhao Li
  • Fei Yin
Original Article


The objective of the study was to explore the pathogenesis of mesial temporal lobe epilepsy (MTLE) and the mechanism of valproate administration in the early stage of MTLE development. We performed a global comparative analysis and function classification of differentially expressed proteins using proteomics. MTLE models of developmental rats were induced by lithium-pilocarpine. Proteins in the hippocampus were separated by 2-DE technology. PDQuest software was used to analyze 2-DE images, and MALDI-TOF-MS was used to identify the differentially expressed proteins. Western blot was used to determine the differential expression levels of synapse-related proteins synapsin-1, dynamin-1 and neurogranin in both MTLE rat and human hippocampus. A total of 48 differentially expressed proteins were identified between spontaneous and non-spontaneous MTLE rats, while 41 proteins between MTLE rats and post valproate-treatment rats were identified. All of the proteins can be categorized into several groups by biological functions: synaptic and neurotransmitter release, cytoskeletal structure and dynamics, cell junctions, energy metabolism and mitochondrial function, molecular chaperones, signal regulation and others. Western blot results were similar to the changes noted in 2-DE. The differentially expressed proteins, especially the proteins related to synaptic and neurotransmitter release function, such as synapsin-1, dynamin-1 and neurogranin, are probably involved in the mechanism of MTLE and the pharmacological effect of valproate. These findings may provide important clues to elucidate the mechanism of chronic MTLE and to identify an optimum medication intervention time and new biomarkers for the development of pharmacological therapies targeted at epilepsy.


Mesial temporal lobe epilepsy Hippocampus Valproate Proteomics Mechanism 



Antiepileptic drugs


Gap junction channel protein connexin 50


Two-dimensional polyacrylamide gel electrophoresis


Actin filaments


Heat shock proteins


Inner molecular layer


Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry


Dual specificity mitogen-activated protein kinase kinase 1


Mesial temporal lobe epilepsy


Status epilepticus


NAD-dependent deacetylase sirtuin-2


T-complex protein 1





We thank Dr. Zhiquan Yang (Department of Neurosurgery, Xiangya Hospital, China) for providing the experimental hippocampus of MTLE patients and Dr. Zhaojun Duan (Department of Clinical Laboratory, Xiangya Hospital, China) for critically reviewing the manuscript. This work was supported by the National Natural Science Foundation of China, 30901631. The authors declare that there is no conflict of interest in either financial support or relationships.


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Liwen Wu
    • 1
  • Jing Peng
    • 1
  • Chaoping Wei
    • 1
  • Gu Liu
    • 1
  • Guoli Wang
    • 1
  • Kongzhao Li
    • 1
  • Fei Yin
    • 1
  1. 1.Department of PediatricsXiangya Hospital, Central South UniversityChangshaPeople’s Republic of China

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