Shotgun strategy-based proteome profiling analysis on the head of silkworm Bombyx mori
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Insect head is comprised of important sensory systems to communicate with internal and external environment and endocrine organs such as brain and corpus allatum to regulate insect growth and development. To comprehensively understand how all these components act and interact within the head, it is necessary to investigate their molecular basis at protein level. Here, the spectra of peptides digested from silkworm larval heads were obtained from liquid chromatography tandem mass spectrometry (LC–MS/MS) and were analyzed by bioinformatics methods. Totally, 539 proteins with a low false discovery rate (FDR) were identified by searching against an in-house database with SEQUEST and X!Tandem algorithms followed by trans-proteomic pipeline (TPP) validation. Forty-three proteins had the theoretical isoelectric point (pI) greater than 10 which were too difficult to separate by two-dimensional gel electrophoresis (2-DE). Four chemosensory proteins, one odorant-binding protein, two diapause-related proteins, and a lot of cuticle proteins, interestingly including pupal cuticle proteins were identified. The proteins involved in nervous system development, stress response, apoptosis and so forth were related to the physiological status of head. Pathway analysis revealed that many proteins were highly homologous with the human proteins which involved in human neurodegenerative disease pathways, probably implying a symptom of the forthcoming metamorphosis of silkworm. These data and the analysis methods were expected to be of benefit to the proteomics research of silkworm and other insects.
KeywordsBombyx mori Insect head Proteomics LTQ-Orbitrap Gene Ontology Pathway
This work was supported by the National Basic Research Program of China (Grant No. 2005CB121003), National Hi-Tech Research and Development Program of China (Grant No. 2006AA10A118), and Program for New Century Excellent Talents in University. We are thankful to the 985-Institute of Agrobiology and Environmental Sciences of Zhejiang University, for providing convenience for our experiment. We are also grateful to Mr. Wei Fan and Jisheng Li for kind help in our data analysis.
Conflict of interest statement
The authors declare that they have no conflict of interest.
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