Amino Acids

, Volume 39, Issue 1, pp 155–160 | Cite as

CSD mRNA expression in rat testis and the effect of taurine on testosterone secretion

  • Jiancheng Yang
  • Gaofeng Wu
  • Ying Feng
  • Changmian Sun
  • Shumei Lin
  • Jianmin HuEmail author
Original Article


In the present study, the cysteine sulfinate decarboxylase (CSD) mRNA expression was detected in rat testis by RT-PCR. The results showed that CSD mRNA was expressed in rat testis, and the putative encoded-amino acid sequence was exactly the same as that in rat liver which was already known. At the same time, the effects of taurine on testosterone secretion were investigated both in vivo and in vitro. In vivo, taurine were administered to male rats by tap water. The results showed that taurine obviously stimulated the secretion of FSH, LH and testosterone in serum, but showed no significant effect on the secretion of estradiol. Taurine administered in water could significantly increase the concentration of taurine in the blood and testis of rats. In vitro, cultured Leydig cells were treated with taurine independently or incubated with human chorionic gonadotropin (HCG) and progesterone. The results showed that taurine had biphasic effects on basal testosterone secretion in cultured Leydig cells. Low concentrations of taurine (0.1–100 μg/ml) could stimulate testosterone secretion, whereas high concentration of taurine (400 μg/ml) could inhibit testosterone secretion. Testosterone secretion stimulated by HCG was significantly increased by 10 and 100 μg/ml of taurine administration, and obviously decreased by treating with 400 μg/ml of taurine. Testosterone secretion induced by progesterone was significantly stimulated by treating with 1.0 and 10 μg/ml of taurine, however, it was significantly inhibited when treated with 400 μg/ml of taurine. Meanwhile, the effect of silencing CSD mRNA by siRNA on testosterone secretion was analyzed. The results showed that testosterone secretion was obviously decreased after the inhibition of CSD mRNA expression in cultured Leydig cells. These results indicated that taurine can be synthesized in rat testis by CSD pathway, and it plays important roles in testosterone secretion both in vivo and in vitro which need to be further investigated.


Taurine CSD mRNA expression Testosterone secretion Leydig cell Rat 



This work was supported by the National Natural Science Foundation of China (Grant No. 30371048) and the Doctor Initial Foundation of Liaoning Province (Grant No. 20081065).


  1. Alvarez JG, Storey BT (1983) Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility. Biol Reprod 29:548–555CrossRefPubMedGoogle Scholar
  2. Bligh J (1981) Amino acids as central synaptic transmitters or modulators in the mammalian thermoregulation. Fed Proc 40:2746–2749PubMedGoogle Scholar
  3. Boatman DE, Bavister DB, Cruz E (1990) Addition of hypotaurine can reactivate immotile golden hamster spermatozoa. J Androl 11:66–72PubMedGoogle Scholar
  4. Dou JT, Li M (1999) The response of leydig cells isolated by Percoll to the stimulation of human chronic gonadotropin. Acad J PLA Postgrad Med Sch 20(3):217–219Google Scholar
  5. Fraser LR (1986) Both taurine and albumin support mouse sperm motility and fertilizing ability in vitro but there is no obligatory requirement for taurine. J Reprod Fertil 77(1):271–280PubMedCrossRefGoogle Scholar
  6. Holmes RP, Goodman HO, Shihabi ZK, Jarrow JP (1992) The taurine and hypotaurine content of human semen. J Androl 13:289–292PubMedGoogle Scholar
  7. Hope Db (1955) Yridoxal phosphate as the coenzyme of the mammalian decarboxylase for l-cysteine sulphinic and l-cysteic acids. Biochem J 59:497–500PubMedGoogle Scholar
  8. Hu JM, Ikemura R, Chang KT, Suzuki M, Nishihara M, Takahashi M (2000) Expression of cysteine sulfinate decarboxylase mRNA in rat mammary gland. J Vet Med Sci 62(8):829–834CrossRefPubMedGoogle Scholar
  9. Huxtable RJ, Bressler R (1973) Effects of taurine on a muscle intracellular membrane. Biochim Biophys Acta 323:573–583CrossRefPubMedGoogle Scholar
  10. Isabelle R, Alain S, Marcel T (1996) Molecular cloning and sequence analysis of the cDNA encoding rat liver cysteine sulfinate decarboxylase (CSD). Biochim Biophys Acta 1307:152–156Google Scholar
  11. Kuriyama K, Muramatsu M, Nakagawa K, Kakita K (1978) Modulating role of taurine on release of neurotransmitters and calcium transport in excitable tissue. In: Barbeau A, Huxtable RJ (eds) Taurine and neurological disorders. Raven Press, New York, pp 201–216Google Scholar
  12. Lasserre P, Gilles R (1971) Modification of the amino acid pool in the parietal muscle of two euryhaline teleosts during osmotic adjustment. Experientia 27:1437–1445CrossRefGoogle Scholar
  13. Li JH, Ling YQ, Fan JJ, Zhang XP, Cui S (2006) Expression of cysteine sulfinate decarboxylase (CSD) in male reproductive organs of mice. Histochem Cell Biol 125:607–613CrossRefPubMedGoogle Scholar
  14. Llanos MN, Ronco AM (1994) Sperm phospholipid methyltransferase activity during preparation for exocytosis. Cell Biochem Funct 12:289–296CrossRefPubMedGoogle Scholar
  15. Lobo MVT, Alonso FJM, and Rafael Martin del Rio (2000) Immunohistochemical localization of taurine in the male reproductive organs of the rat. J Histochem Cytochem, 48:313–320Google Scholar
  16. Magnusson KR (1994) Changes in the localization of taurine-like immunoreactivity during development and regeneration in the rat brain. In: Huxtable R, Michalk DV (eds) Taurine in health and disease. Plenum Press, New York, pp 235–243Google Scholar
  17. Meizel S (1985) Molecules that initiate or help stimulate the acrosome reaction by their interaction with the mammalian sperm surface. Am J Anat 174:285–302CrossRefPubMedGoogle Scholar
  18. Mrsny RJ, Meizel S (1985) Inhibition of hamster sperm Na+, K+-ATPase activity by taurine and hypotaurine. Life Sci 36:271–275CrossRefPubMedGoogle Scholar
  19. Oertel WH, Schmechel DE, Weise VK, Ransom DH, Tappaz ML, Krutzsch HC, Kopin IJ (1981) Comparison of cysteine sulphinic acid decarboxylase isoenzymes and glutamic acid decarboxylase in rat liver and brain. Neuroscience 6:2701–2714CrossRefPubMedGoogle Scholar
  20. Park E, Park SY, Wang C, Xu J, LaFauci G, Schuller-Levis G (2002) Cloning of murine cysteine sulfinic acid decarboxylase and its mRNA expression in murine tissues. Biochim Biophys Acta 1574(3):403–406PubMedGoogle Scholar
  21. Pasantes-Morales H, Lopez-colome AM, Salceda R, Mandel P (1976) Cysteine sulphinate decarboxylase in chick and rat retina during development. J Neurochem 27:1103–1106CrossRefPubMedGoogle Scholar
  22. Read WO, Welty JD (1963) Effects of taurine on epinephrine and digoxin-irregularities of dog heart. J Pharmacol Exp Ther 139:283–289PubMedGoogle Scholar
  23. Tiedemann F, Gmelin L (1827) (1827) Einige neue bestandtheile der galle des ochsen. Physic Chem 9:326–337Google Scholar
  24. Timbrell JA, Seabra V, Waterfield CJ (1995) The in vivo and in vivo protective properties of taurine. General Pharmacol 26:453–462Google Scholar
  25. Velazquez A, Delgado NM, Rosado A (1986) Taurine content and amino acid composition of human acrosome. Life Sci 38:991–995CrossRefPubMedGoogle Scholar
  26. Vessey DA (1978) The biochemical basis for the conjugation of bile acids with either glycine or taurine. Biochem J 174:621–626PubMedGoogle Scholar
  27. Yang JC, Feng Y, Sun CM, Hu JM (2007) Effect of taurine on the secretion of reproduction hormone of male rat. J Anhui Agri Sci 35(11):3283–3284Google Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Jiancheng Yang
    • 1
  • Gaofeng Wu
    • 1
  • Ying Feng
    • 1
  • Changmian Sun
    • 2
  • Shumei Lin
    • 1
  • Jianmin Hu
    • 1
    Email author
  1. 1.College of Animal Science and Veterinary MedicineShenyang Agricultural UniversityShenyangChina
  2. 2.College of Animal ScienceChina Agricultural UniversityBeijingChina

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