Amino Acids

, Volume 38, Issue 3, pp 763–769 | Cite as

Connective tissue growth factor is a downstream mediator for preptin-induced proliferation and differentiation in human osteoblasts

Original Article

Abstract

Preptin, a newly isolated 34-amino-acid peptide hormone that is cosecreted with insulin and amylin from pancreatic beta-cells, has emerged as a regulatory element in bone metabolism, but its mechanism remains unclear. We assessed the effects of preptin on proliferation and differentiation of human osteoblasts and investigated the mechanism involved. Our results demonstrated that preptin promoted human osteoblasts proliferation and alkaline phosphatase activity. Suppression of connective tissue growth factor (CTGF), which was upregulated by preptin in a dose- and time-dependent manner, with small interfering RNA (siRNA) abolished the preptin-induced human osteoblasts proliferation and differentiation. Preptin induced activation of ERK mitogen-activated protein kinase (MAPK), but not p38 or JNK in human osteoblasts. Furthermore, pretreatment of human osteoblasts with the ERK inhibitor PD98059 abolished the preptin-induced CTGF secretion and blocked the promoting effect of preptin on osteoblasts proliferation and differentiation. These data demonstrated that preptin is involved in bone anabolism mediated by ERK/CTGF in human osteoblasts and may contribute to the preservation of bone mass observed in hyperinsulinemic states, such as obesity.

Keywords

Preptin Connective tissue growth factor Osteoblasts Extracellular signal regulated kinase 

Notes

Acknowledgments

This work was supported by Grant from Chinese National Key Basic ‘973’ Research Project (No. 2006CB503808), and the National Natural Science Foundation of China (No. 30801174, No. 30871191 and No. 30572078).

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • You-Shuo Liu
    • 1
    • 2
  • Ying Lu
    • 1
  • Wei Liu
    • 1
  • Hui Xie
    • 1
  • Xiang-Hang Luo
    • 1
  • Xian-Ping Wu
    • 1
    • 2
  • Ling-Qing Yuan
    • 1
  • Er-Yuan Liao
    • 1
  1. 1.Institute of Metabolism and EndocrinologyThe Second Xiang-Ya Hospital, Central South UniversityHunanPeople’s Republic of China
  2. 2.Geriatric Department, The Second Xiang-Ya HospitalCentral South UniversityHunanPeople’s Republic of China

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