Identification of flooding stress responsible cascades in root and hypocotyl of soybean using proteome analysis
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Flooding inducible proteins were analyzed using a proteomic technique to understand the mechanism of soybean response to immersion in water. Soybeans were germinated for 2 days, and then subjected to flooding for 2 days. Proteins were extracted from root and hypocotyl, separated by two-dimensional polyacrylamide gel electrophoresis, stained by Coomassie brilliant blue, and analyzed by protein sequencing and mass spectrometry. Out of 803 proteins, 21 proteins were significantly up-regulated, and seven proteins were down-regulated by flooding stress. Of the total, 11 up-regulated proteins were classified as related to protein destination/storage and three proteins to energy, while four down-regulated proteins were related to protein destination/storage and three proteins to disease/defense. The expression of 22 proteins significantly changed within 1 day after flooding stress. The effects of flooding, nitrogen substitution without flooding, or flooding with aeration were analyzed for 1–4 days. The expression of alcohol dehydrogenase increased remarkably by nitrogen substitution compared to flooding. The expression of many proteins that changed due to flooding showed the same tendencies observed for nitrogen substitution; however, the expression of proteins classified into protein destination/storage did not.
KeywordsFlooding Proteome Soybean Hypoxic response
Two-dimensional polyacrylamide gel electrophoresis
Coomassie brilliant blue
Immobilized pH gradient
This work was supported by grants from National Agriculture and Food Research Organization, Japan. The authors thank Dr. S. Kuroda for his kind support of our research. We also thank Dr. S. Shimamura, Dr. N. Nakayama, Dr. R. Yamamoto and Dr. T. Nakamura for their valuable discussion.
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