Amino Acids

, Volume 36, Issue 3, pp 563–569 | Cite as

Molecularly imprinted polymers for RGD selective recognition and separation

  • Emmanuel Papaioannou
  • Christos Koutsas
  • Maria Liakopoulou-KyriakidesEmail author
Original Article


Molecularly imprinted polymers that could recognize the tripeptide Arg-Gly-Asp have been produced with the use of two functional monomers and three different cross-linkers, respectively. Methacrylic acid and acrylamide were used as functional monomers and the role of the ethylene glycol dimethacrylate, trimethylpropane trimethacrylate and N,N′-methylene-bisacrylamide as crosslinking monomers, was investigated on their recognition capability. The % net rebinding and the imprinting factor values were obtained, giving for the methacrylic acid–trimethylpropane trimethacrylate polymer the highest values 12.3% and 2.44, respectively. In addition, this polymer presented lower dissociation constant (K D) value and the higher B max% of theoretical total binding sites than all the other polymers. Rebinding experiments with Lys-Gly-Asp, an analogue of Arg-Gly-Asp, and other different peptides, such as cholecystokinin C-terminal tri- and pentapeptide and gramicidin, further indicated the selectivity of methacrylic acid-trimethylpropane trimethacrylate copolymer for Arg-Gly-Asp giving specific selectivity factor values 1.27, 1.98, 1.31 and 1.67, respectively.


Arg-Gly-Asp RGD Copolymerization Molecular imprinting Radical polymerization 





Arg-Gly-Asp tripeptide


Lys-Gly-Asp tripeptide

CCK-3, CCK-5

Cholecystokinin C-terminal tripeptide and pentapeptide, respectively


Ethylene glycol dimethacrylate


Trimethylpropane trimethacrylate


Methacrylic acid


Molecularly imprinted polymers


Non imprinted polymers


Scanning electron microscopy


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Emmanuel Papaioannou
    • 1
  • Christos Koutsas
    • 1
  • Maria Liakopoulou-Kyriakides
    • 1
    Email author
  1. 1.Section of Chemistry, Faculty of Chemical EngineeringAristotle University of ThessalonikiThessalonikiGreece

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