Amino Acids

, Volume 34, Issue 1, pp 25–33

Differences in amino acids composition and coupling patterns between mesophilic and thermophilic proteins

Review Article


Thermophilic proteins show substantially higher intrinsic thermal stability than their mesophilic counterparts. Amino acid composition is believed to alter the intrinsic stability of proteins. Several investigations and mutagenesis experiment have been carried out to understand the amino acid composition for the thermostability of proteins. This review presents some generalized features of amino acid composition found in thermophilic proteins, including an increase in residue hydrophobicity, a decrease in uncharged polar residues, an increase in charged residues, an increase in aromatic residues, certain amino acid coupling patterns and amino acid preferences for thermophilic proteins. The differences of amino acids composition between thermophilic and mesophilic proteins are related to some properties of amino acids. These features provide guidelines for engineering mesophilic protein to thermophilic protein.

Keywords: Thermophilic protein – Thermostability – Amino acid composition – Amino acid coupling pattern 


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Institute of Chemical Biology and Pharmaceutical ChemistryZhejiang UniversityHangzhouChina
  2. 2.Food Quality and Safety DepartmentZhejiang Gongshang UniversityHangzhouChina
  3. 3.Microbiology Division, College of Animal ScienceZhejiang UniversityHangzhouChina

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