Amino Acids

, Volume 32, Issue 3, pp 347–357 | Cite as

Mass spectrometric analysis of protein histidine phosphorylation

  • X.-L. Zu
  • P. G. Besant
  • A. Imhof
  • P. V. Attwood


Protein histidine phosphorylation is now recognized as an important form of post-translational modification. The acid-lability of phosphohistidine has meant that this phosphorylation has not been as well studied as serine/threonine or tyrosine phosphorylation. We show that phosphohistidine and phosphohistidine-containing phosphopeptides derived from proteolytic digestion of phosphohistone H4 are detectable by ESI-MS. We also demonstrate reverse-phase HPLC separation of these phosphopeptides and their detection by MALDI-TOF-MS.

Keywords: Phosphohistidine – Mass spectrometry – Phosphoamino acid analysis – Histone H4 – Phosphopeptide – Histidine kinase 


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • X.-L. Zu
    • 1
  • P. G. Besant
    • 1
  • A. Imhof
    • 2
  • P. V. Attwood
    • 1
  1. 1.School of Biomedical, Biomolecular and Chemical Sciences (M310)The University of Western AustraliaCrawleyAustralia
  2. 2.Department of Molecular Biology, Adolf-Butenandt Institute, Histone Modifications GroupLudwig-Maximilians-University of MunichMunichGermany

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