Influence of β-alanine supplementation on skeletal muscle carnosine concentrations and high intensity cycling capacity
Article First Online: 28 July 2006 Received: 14 March 2006 Accepted: 20 April 2006 Summary.
Muscle carnosine synthesis is limited by the availability of β-alanine. Thirteen male subjects were supplemented with β-alanine (CarnoSyn™) for 4 wks, 8 of these for 10 wks. A biopsy of the
vastus lateralis was obtained from 6 of the 8 at 0, 4 and 10 wks. Subjects undertook a cycle capacity test to determine total work done (TWD) at 110% (CCT 110%) of their maximum power (W max). Twelve matched subjects received a placebo. Eleven of these completed the CCT 110% at 0 and 4 wks, and 8, 10 wks. Muscle biopsies were obtained from 5 of the 8 and one additional subject. Muscle carnosine was significantly increased by +58.8% and +80.1% after 4 and 10 wks β-alanine supplementation. Carnosine, initially 1.71 times higher in type IIa fibres, increased equally in both type I and IIa fibres. No increase was seen in control subjects. Taurine was unchanged by 10 wks of supplementation. 4 wks β-alanine supplementation resulted in a significant increase in TWD (+13.0%); with a further +3.2% increase at 10 wks. TWD was unchanged at 4 and 10 wks in the control subjects. The increase in TWD with supplementation followed the increase in muscle carnosine. Keywords: β-alanine – Muscle – Carnosine – Taurine – Exercise – Cycle performance References Abe, H 2000 Role of histidine-related compounds as intracellular proton buffering constituents in vertebrate muscle Biochemistry (Mosc) 65 757 765 Google Scholar Asatoor, AM, Bardon, JK, Lant, AF, Milne, MD, Navab, F 1970 Intestinal absorption of carnosine and its constituent amino acids in man Gut 11 250 254 PubMed Google Scholar Bakardjiev, A, Bauer, K 1994 Transport of β-alanine and biosynthesis of carnosine by skeletal muscle cells in primary culture Eur J Biochem 225 617 623 PubMed CrossRef Google Scholar Bate-Smith, EC 1938 The buffering of muscle in rigor: protein, phosphate and carnosine J Physiol (Lond) 92 336 343 Google Scholar Batrukova, MA, Rubtsov, AM 1997 Histidine-containing dipeptides as endogenous regulators of the activity of sarcoplasmic reticulum Ca-release channels Biochem Biophys Acta 1324 142 150 PubMed Google Scholar Bell, GJ, Wenger, HA 1988 The effect of one-legged sprint training on intramuscular pH and non-bicarbonate buffering capacity Eur J Appl Physiol 58 158 164 CrossRef Google Scholar Bergström, J 1962 Muscle electrolytes in man. Determined by neutron activation analysis on needle biopsy specimens. A study on normal subjects, kidney patients and patients with chronic diarrhoea Scand J Clin Lab Invest 14 1 110 Google Scholar Bishop, D, Edge, J, Goodman, C 2004 Muscle buffer capacity and aerobic fitness are associated with repeated-sprint ability in women Eur J Appl Physiol 92 540 547 PubMed CrossRef Google Scholar Boldyrev, AA, Koldobski, A, Kurella, E, Maltseva, V, Stvolinski, S 1993 Natural histidine-containing dipeptide carnosine as a potent hydrophilic antioxidant with membrane stabilizing function. A biomedical aspect Mol Chem Neuropathol 19 185 192 PubMed CrossRef Google Scholar Chin, ER, Allen, DG 1998 The contribution of pH-dependent mechanisms to fatigue at different intensities in mammalian single muscle fibres J Physiol (Lond) 512 831 840 CrossRef Google Scholar Crush, KG 1970 Carnosine and related substances in animal tissues Comp Biochem Physiol 34 3 30 PubMed CrossRef Google Scholar Dawson, R, Biasetti, M, Messina, S, Dominy, J 2002 The cytoprotective role of taurine in exercise-induced muscle injury Amino Acids 22 309 324 PubMed CrossRef Google Scholar Dunnett, M, Harris, RC 1995 Carnosine & Taurine contents of different fibre types in the middle gluteal muscle of the Thoroughbred horse Equine Vet J 18 214 217 Google Scholar Dunnett, M, Harris, RC 1997 High-performance liquid chromatographic determination of imidazole dipeptides, histidine, 1-methylhistidine and 3-methylhistidine in equine & camel muscle & individual muscle fibres J Chromatogr B 688 47 55 Google Scholar Dunnett, M, Harris, RC, Soliman, MZ, Suwar, AAS 1997 Carnosine, anserine and taurine contents in individual fibres from the middle gluteal muscle of the camel Res Vet Sci 62 213 216 PubMed CrossRef Google Scholar Dunnett, M, Harris, RC 1999 Influence of oral β-alanine and L-histidine supplementation on the carnosine content of the gluteus medius Equine Vet J 30 499 504 Google Scholar Edge, J, Bishop, , Hill-Haas, S, Dawson, B, Goodman, C 2006 Comparison of muscle buffer capacity and repeated-sprint ability of untrained endurance-trained and team-sport athletes Eur J Appl Physiol 96 225 234 CrossRef Google Scholar Fabiato, A, Fabiato, F 1978 Effects of pH on the myofilaments and the sarcoplasmic reticulum of skinned cells from the cardiac and skeletal muscles J Physiol (Lond) 276 233 255 Google Scholar Favero, TG, Zable, AC, Bowman, M, Thompson, A, Abramson, JJ 1995 Metabolic end products inhibit sarcoplasmic reticulum Ca 2+ release and [H]ryanodine binding J Appl Physiol 78 1665 1667 PubMed Google Scholar Harris, RC, Edwards, RHT, Hultman, E, Nordesjo, LO, Nylind, B 1976 The time course of phosphorylcreatine resynthesis during recovery of the quadriceps muscle in man Pflugers Arch 367 137 142 PubMed CrossRef Google Scholar Harris, RC, Dunnett, M, Greenhaff, PL 1998 Carnosine & taurine contents in individual fibres in human vastus lateralis muscle J Sports Sci 16 639 643 CrossRef Google Scholar Harris, RC, Söderlund, K, Hultman, E 1992 Elevation of creatine in resting and exercised muscle in normal subjects by creatine supplementation Clin Sci 83 367 374 PubMed Google Scholar Harris, RC, Katz, A, Sahlin, K, Snow, DH 1989 Effect of freeze drying on measurements of pH in biopsy samples of the middle gluteal muscle of the horse: comparison of muscle pH to the pyruvate and lactate content Equine Vet J 21 45 47 PubMed CrossRef Google Scholar Harris, RC, Marlin, DJ, Dunnett, M, Snow, DH, Hultman, E 1990 Muscle buffering capacity & dipeptide content in the thorough bred horse, greyhound dog & man Comp Biochem Physiol 97A 249 251 CrossRef Google Scholar Harris, RC, Tallon, MJ, Dunnett, M, Boobis, L, Coakley, J, Kim, HJ, Fallowfield, JL, Hill, CA, Sale, C, Wise, JA 2006 The absorption of orally supplied β-alanine and its effect on muscle carnosine synthesis in human vastus lateralis Amino Acids 30 279 289 PubMed CrossRef Google Scholar Hipkiss, AR 2000 Carnosine and protein carbonyl groups: a possible relationship Biochemistry (Mosc) 65 771 778 Google Scholar Hipkiss, AR, Michaelis, J, Syrris, P 1995 Non-enzymatic glycosylation of the dipeptide L-carnosine, a potential anti-protein-cross-linking agent FEBS Lett 371 81 85 PubMed CrossRef Google Scholar Hultman, E, Sahlin, K 1980 Acid-base balance during exercise Exerc Sport Sci Rev 8 41 128 PubMed Google Scholar Jessen, H 1994 Taurine and beta-alanine transport in an established human kidney cell line derived from the proximal tubule Biochim Biophys Acta 1194 44 52 PubMed CrossRef Google Scholar Juel, C, Lundby, C, Sander, M, Calbet, JAL, van Hall, G 2003 Human skeletal muscle and erythrocyte proteins involved in acid-base homeostasis: adaptations to chronic hypoxia J Physiol 548 639 648 PubMed CrossRef Google Scholar Lamont, C, Miller, DJ 1992 Calcium sensitizing action of carnosine and other endogenous imidazoles in chemically skinned striated muscle J Physiol 454 421 434 PubMed Google Scholar Mannion, AF, Jakeman, PM, Dunnett, M, Harris, RC, Willan, PLT 1992 Carnosine and anserine concentrations in the quadriceps femoris muscle of healthy humans Eur J Appl Physiol 64 47 50 CrossRef Google Scholar Meyerhoff, O 1920 Die Engergieumwandlungen im Muskel. III. Kohlenhydrat-und Milchsäureumsatz im Froschmuskel Pflugers Arch Ges Physiol 185 11 32 CrossRef Google Scholar Nevill, ME, Boobis, LS, Brooks, S, Williams, C 1989 Effect of training on muscle metabolism during treadmill sprinting J Appl Physiol 67 2376 2382 PubMed Google Scholar Parkhouse, WS, McKenzie, DC, Hochachka, PW, Ovalle, WK 1985 Buffering capacity of deproteinized human vastus lateralis muscle J Appl Physiol 58 14 17 PubMed Google Scholar Perry, TL, Hansen, S, Tischler, B, Bunting, R, Berry, K 1967 Carnosinemia: a new metabolic disorder associated with neurologic disease and mental defect New Engl J Med 277 1219 1226 PubMed CrossRef Google Scholar Ramamoorthy, S, Leibach, FH, Mahesh, VB, Han, H, Yang-Feng, T, Blakely, RD, Ganapathy, V 1994 Functional characterization and chromosomal localization of a cloned taurine transporter from human placenta Biochem J 300 893 900 PubMed Google Scholar Sewell, DA, Harris, RC, Marlin, DJ, Dunnett, M 1992 Estimation of the carnosine content of different fibre types in the middle gluteal muscle of the thoroughbred horse J Physiol 455 447 453 PubMed Google Scholar Spriet, LL, Lindinger, MI, McKelvie, RS, Heigenhauser, GJF, Jones, NL 1989 Muscle glycogenolysis and H + concentration during maximal intermittent cycling J Appl Physiol 66 8 13 PubMed Google Scholar Susuki, Y, Ito, O, Takahashi, H, Takamatsu, K 2004 The effect of sprint training on skeletal muscle carnosine in humans Int J Sport Health Sci 2 105 110 Google Scholar Suzuki, Y, Ito, O, Mukai, N, Takahashi, H, Takamatsu, K 2002 High level of skeletal muscle carnosine contributes to the latter half of exercise performance during 30-s maximal cycle ergometer sprinting Jpn J Physiol 52 199 205 PubMed CrossRef Google Scholar Tallon, MJ, Harris, RC, Boobis, L, Fallowfield, J, Wise, JA 2005 The carnosine content of vastus lateralis is elevated in resistance trained bodybuilders J Strength Condit Res 19 725 729 CrossRef Google Scholar Talmadge, RJ, Roy, RR 1993 Electrophoretic separation of rat skeletal muscle myosin heavy-chain isoforms J Appl Physiol 75 2337 2340 PubMed Google Scholar Trivedi, B, Danforth, WH 1966 Effect of pH on the kinetics of frog muscle phosphofructokinase J Biol Chem 241 4110 4112 PubMed Google Scholar Wade, CE, Miller, MM, Baer, LA, Moran, MM, Steele, MK, Stein, TP 2002 Body mass, energy intake, and water consumption of rats and humans during space flight Nutrition 18 829 836 PubMed CrossRef Google Scholar Westerblad, H, Bruton, JD, Lannergren, J 1997 The effect of intracellular pH on contractile function of intact, single fibres of mouse muscle declines with increasing temperature J Physiol 500 193 204 PubMed Google Scholar Weston, AR, Myburgh, KH, Lindsay, FH, Dennis, SC, Noakes, TD, Hawley, JA 1997 Skeletal muscle buffering capacity and endurance performance after high-intensity interval training by well-trained cyclists Eur J Appl Physio l75 7 13 Google Scholar