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The absorption of orally supplied β-alanine and its effect on muscle carnosine synthesis in human vastus lateralis

Summary.

β-Alanine in blood-plasma when administered as A) histidine dipeptides (equivalent to 40 mg · kg−1 bwt of β-alanine) in chicken broth, or B) 10, C) 20 and D) 40 mg · kg−1 bwt β-alanine (CarnoSyn™, NAI, USA), peaked at 428 ± SE 66, 47 ± 13, 374 ± 68 and 833 ± 43 µM. Concentrations regained baseline at 2 h. Carnosine was not detected in plasma with A) although traces of this and anserine were found in urine. Loss of β-alanine in urine with B) to D) was <5%. Plasma taurine was increased by β-alanine ingestion but this did not result in any increased loss via urine. Pharmacodynamics were further investigated with 3 × B) per day given for 15 d. Dietary supplementation with I) 3.2 and II) 6.4 g · d−1 β-alanine (as multiple doses of 400 or 800 mg) or III) L-carnosine (isomolar to II) for 4 w resulted in significant increases in muscle carnosine estimated at 42.1, 64.2 and 65.8%.

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References

  1. H Abe (2000) ArticleTitleRole of histidine-related compounds as intracellular proton buffering constituents in vertebrate muscle Biochemistry (Mosc) 65 757–765 Occurrence Handle1:CAS:528:DC%2BD3cXlvFOlsb4%3D

  2. AM Asatoor JK Bardon AF Lant MD Milne F Navab (1970) ArticleTitleIntestinal absorption of carnosine and its constituent amino acids in man Gut 11 250–254 Occurrence Handle1:CAS:528:DyaE3cXktleitrk%3D Occurrence Handle5423906

  3. A Bakardjiev K Bauer (1994) ArticleTitleTransport of β-alanine and biosynthesis of carnosine by skeletal muscle cells in primary culture Eur J Biochem 225 617–623 Occurrence Handle1:CAS:528:DyaK2cXmt1Ojtb0%3D Occurrence Handle7957176 Occurrence Handle10.1111/j.1432-1033.1994.00617.x

  4. EC Bate-Smith (1938) ArticleTitleThe buffering of muscle in rigor: protein, phosphate and carnosine J Physiol 92 336–343

  5. MA Batrukova AM Rubtsov (1997) ArticleTitleHistidine-containing dipeptides as endogenous regulators of the activity of sarcoplasmic reticulum Ca-release channels Biochem Biophys Acta 1324 142–150 Occurrence Handle1:CAS:528:DyaK2sXpt1yhtQ%3D%3D Occurrence Handle9059507

  6. J Bergström (1962) ArticleTitleMuscle electrolytes in man. Determined by neutron activation analysis on needle biopsy specimens. A study on normal subjects, kidney patients and patients with chronic diarrhoea Scand J Clin Lab Invest 14 1–110

  7. AA Boldyrev A Koldobski E Kurella V Maltseva S Stvolinski (1993) ArticleTitleNatural histidine-containing dipeptide carnosine as a potent hydrophilic antioxidant with membrane stabilizing function. A biomedical aspect Mol Chem Neuropathol 19 185–192 Occurrence Handle1:CAS:528:DyaK3sXltlSktrw%3D Occurrence Handle8363703 Occurrence Handle10.1007/BF03160178

  8. WK Chan EA Decker CK Chow GA Boissonneault (1994) ArticleTitleEffect of dietary carnosine on plasma and tissue antioxidant concentrations and on lipid oxidation in rat skeletal muscle Lipids 29 461–466 Occurrence Handle1:CAS:528:DyaK2cXltVOhs7k%3D Occurrence Handle7968266

  9. R Dawson SuffixJr M Biasetti S Messina J Dominy (2002) ArticleTitleThe cytoprotective role of taurine in exercise-induced muscle injury Amino Acids 22 309–324 Occurrence Handle1:CAS:528:DC%2BD38XmvFCqt74%3D Occurrence Handle12107759 Occurrence Handle10.1007/s007260200017

  10. ST Dieck H Heuer J Ehrchen C Otto K Bauer (1999) ArticleTitleThe peptide transporter PepT2 is expressed in rat brain and mediates the accumulation of the fluorescent dipeptide derivative beta-Ala-Lys-Nepsilon-AMCA in astrocytes Glia 25 10–20 Occurrence Handle1:STN:280:DyaK1M7gtleluw%3D%3D Occurrence Handle9888294 Occurrence Handle10.1002/(SICI)1098-1136(19990101)25:1<10::AID-GLIA2>3.0.CO;2-Y

  11. Dunnett M (1995) Carnosine metabolism and function in the thoroughbred horse. PhD thesis, Open University

  12. M Dunnett RC Harris (1995) ArticleTitleCarnosine and taurine contents of different fibre types in the middle gluteal muscle of the Thoroughbred horse Equine Vet J [Suppl] 18 214–217

  13. M Dunnett RC Harris (1997) ArticleTitleHigh-performance liquid chromatographic determination of imidazole dipeptides, histidine, 1-methylhistidine and 3-methylhistidine in equine and camel muscle and individual muscle fibres J Chromatogr B 688 47–55 Occurrence Handle1:CAS:528:DyaK2sXksVSitQ%3D%3D

  14. M Dunnett RC Harris MZ Soliman AAS Suwar (1997) ArticleTitleCarnosine, anserine and taurine contents in individual fibres from the middle gluteal muscle of the camel Res Vet Sci 62 213–216 Occurrence Handle1:CAS:528:DyaK2sXmt1WrtLo%3D Occurrence Handle9300536 Occurrence Handle10.1016/S0034-5288(97)90192-2

  15. M Dunnett RC Harris (1999) ArticleTitleInfluence of oral β-alanine and L-histidine supplementation on the carnosine content of the gluteus medius Equine Vet J [Suppl] 30 499–504 Occurrence Handle1:STN:280:DC%2BD3c7isVGgsA%3D%3D

  16. M Dunnett RC Harris CE Dunnett PA Harris (2002) ArticleTitlePlasma carnosine concentration: diurnal variation and effects of age, exercise and muscle damage Equine Vet J [Suppl] 34 283–287

  17. MLG Gardner KM Illingworth J Kelleher D Wood (1991) ArticleTitleIntestinal absorption of the intact peptide carnosine in man, and comparison with intestinal permeability to lactulose J Physiol 439 411–422 Occurrence Handle1:CAS:528:DyaK3MXksVKmtb0%3D Occurrence Handle1910085

  18. T Hama N Tamaki F Miyamoto M Kita F Tsunemori (1976) ArticleTitleIntestinal absorption of β-alanine, anserine and carnosine in rats J Nutr Sci Vitaminol 22 147–157 Occurrence Handle1:CAS:528:DyaE28Xlt1SgtL0%3D Occurrence Handle956928

  19. H Harada SN Allo J Viyuoh AK Takahashi SW Schaffer (1988) ArticleTitleRegulation of calcium transport in drug-induced taurine-depleted hearts Biochim Biophys Acta 944 273–278 Occurrence Handle1:CAS:528:DyaL1cXlvVyjtrY%3D Occurrence Handle2972316

  20. RC Harris HJ Kim DB Harris CA Hill CK Kim L Boobis C Sale JA Wise (2005) ArticleTitleThe distribution of carnosine and taurine in different muscle fibre types from human v lateralis and the effects of beta-alanine supplementation Amino Acids 29 13

  21. RC Harris M Dunnett PL Greenhaff (1998) ArticleTitleCarnosine and taurine contents in individual fibres in human vastus lateralis muscle J Sports Science 16 639–643 Occurrence Handle10.1080/026404198366443

  22. RC Harris A Katz K Sahlin DH Snow (1989) ArticleTitleEffect of freeze drying on measurements of pH in biopsy samples of the middle gluteal muscle of the horse: Comparison of muscle pH to the pyruvate and lactate content Equine Vet J 21 45–47 Occurrence Handle1:STN:280:DyaL1M7ltlSgtg%3D%3D Occurrence Handle2920700 Occurrence Handle10.1111/j.2042-3306.1989.tb02088.x

  23. RC Harris DJ Marlin M Dunnett DH Snow E Hultman (1990) ArticleTitleMuscle buffering capacity and dipeptide content in the thoroughbred horse, greyhound dog and man Comp Biochem Physiol 97A 249–251 Occurrence Handle1:CAS:528:DyaK3MXktVSi Occurrence Handle10.1016/0300-9629(90)90180-Z

  24. RC Harris K Söderlund E Hultman (1992) ArticleTitleElevation of creatine in resting and exercised muscle in normal subjects by creatine supplementation Clin Sci 83 367–374 Occurrence Handle1:CAS:528:DyaK3sXlvVKjtQ%3D%3D Occurrence Handle1327657

  25. AR Hipkiss (2000) ArticleTitleCarnosine and protein carbonyl groups: a possible relationship Biochemistry (Mosc) 65 771–778 Occurrence Handle1:CAS:528:DC%2BD3cXlvFOlsbw%3D

  26. AR Hipkiss J Michaelis P Syrris (1995) ArticleTitleNon-enzymatic glycosylation of the dipeptide L-carnosine, a potential anti-protein-cross-linking agent FEBS Lett 371 81–85 Occurrence Handle1:CAS:528:DyaK2MXotFCltrw%3D Occurrence Handle7664889 Occurrence Handle10.1016/0014-5793(95)00849-5

  27. AM Hoffmann A Bakardjiev K Bauer (1996) ArticleTitleCarnosine-synthesis in cultures of rat glial cells is restricted to oligodendrocytes and carnosine uptake to astrocytes Neurosci Lett 215 29–32 Occurrence Handle1:CAS:528:DyaK28XlslCqu7g%3D Occurrence Handle8880746 Occurrence Handle10.1016/S0304-3940(96)12937-2

  28. H Horinishi M Grillo FL Margolis (1978) ArticleTitlePurification and characterization of carnosine synthetase from mouse olfactory bulbs J Neurochem 31 909–919 Occurrence Handle1:CAS:528:DyaE1MXhsVOntbs%3D Occurrence Handle100576

  29. HJ Kim J Cho CK Kim RC Harris DB Harris C Sale JA Wise (2005) ArticleTitleEffect on muscle fibre morphology and carnosine content after 12 days training of Korean speed skaters Med Sci Sports Exerc 37 IssueID[Suppl 5] S192 Occurrence Handle10.1097/00005768-200505001-00986

  30. C Lamont DJ Miller (1992) ArticleTitleCalcium sensitizing action of carnosine and other endogenous imidazoles in chemically skinned striated muscle J Physiol 454 421–434 Occurrence Handle1:CAS:528:DyaK38Xks1Ogu7w%3D Occurrence Handle1474497

  31. AF Mannion PM Jakeman M Dunnett RC Harris PLT Willan (1992) ArticleTitleCarnosine and anserine concentrations in the quadriceps femoris muscle of healthy humans Eur J Appl Physiol 64 47–50 Occurrence Handle1:STN:280:DyaK387jslajtw%3D%3D Occurrence Handle10.1007/BF00376439

  32. MM Matthews TW Traut (1987) ArticleTitleRegulation of N-carbamoyl-beta-alanine amidohydrolase, the terminal enzyme in pyrimidine catabolism, by ligand-induced change in polymerization J Biol Chem 262 7232–7237 Occurrence Handle1:CAS:528:DyaL2sXktF2ru7c%3D Occurrence Handle3108250

  33. LM Maynard GA Boissonneault CK Chow CG Bruckner (2001) ArticleTitleHigh level of dietary carnosine are associated with increased concentrations of carnosine and histidine in rat soleus muscle J Nutr 131 287–290 Occurrence Handle1:CAS:528:DC%2BD3MXht1Sju7Y%3D Occurrence Handle11160547

  34. RH Ng FD Marshall (1978) ArticleTitleRegional and subcellular distribution of homocarnosine-carnosine synthetase in the central nervous system of rats J Neurochem 30 187–190 Occurrence Handle1:CAS:528:DyaE1cXhsV2nurk%3D

  35. YJ Park SL Volpe EA Decker (2005) ArticleTitleQuantitation of carnosine in humans plasma after dietary consumption of beef J Agric Food Chem 53 4736–4739 Occurrence Handle1:CAS:528:DC%2BD2MXjslKls7k%3D Occurrence Handle15941308 Occurrence Handle10.1021/jf047934h

  36. WS Parkhouse DC McKenzie PW Hochachka WK Ovalle (1985) ArticleTitleBuffering capacity of deproteinized human vastus lateralis muscle J Appl Physiol 58 14–17 Occurrence Handle1:STN:280:DyaL2M7gsVGmtw%3D%3D Occurrence Handle3968004

  37. TL Perry S Hansen B Tischler R Bunting K Berry (1967) ArticleTitleCarnosinemia: a new metabolic disorder associated with neurologic disease and mental defect N Engl J Med 277 1219–1226 Occurrence Handle1:STN:280:DyaF1c%2FkslOqsA%3D%3D Occurrence Handle6058610 Occurrence Handle10.1056/NEJM196712072772302

  38. DA Sewell RC Harris DJ Marlin M Dunnett (1992) ArticleTitleEstimation of the carnosine content of different fibre types in the middle gluteal muscle of the thoroughbred horse J Physiol 455 447–453 Occurrence Handle1:CAS:528:DyaK38Xlt1ymtbw%3D Occurrence Handle1484359

  39. SD Skaper S Das FD Marshall (1973) ArticleTitleSome properties of a homocarnosine-carnosine synthetase isolated from rat brain J Neurochem 21 1429–1445 Occurrence Handle1:CAS:528:DyaE2cXpt1Gnsw%3D%3D Occurrence Handle4358880

  40. Y Suzuki O Ito N Mukai H Takahashi K Takamatsu (2002) ArticleTitleHigh level of skeletal muscle carnosine contributes to the latter half of exercise performance during 30-s maximal cycle ergometer sprinting Jpn J Physiol 52 199–205 Occurrence Handle1:CAS:528:DC%2BD38Xmt1ait7o%3D Occurrence Handle12139778 Occurrence Handle10.2170/jjphysiol.52.199

  41. Y Suzuki O Ito H Takahashi K Takamatsu (2004) ArticleTitleThe effect of sprint training on skeletal muscle carnosine in humans Int J Sport Health Sci 2 105–110

  42. MJ Tallon RC Harris L Boobis J Fallowfield JA Wise (2005) ArticleTitleThe carnosine content of vastus lateralis is elevated in resistance trained bodybuilders J Strength Condit Res 19 725–729 Occurrence Handle10.1519/041018.1

  43. N Tamaki A Funatsuka S Fujimoto T Hama (1984) ArticleTitleThe utilization of carnosine in rats fed on a histidine-free diet and its effect on the levels of tissue histidine and carnosine J Nutr Sci Vitaminol 30 545–551

  44. N Tamaki A Funatsuka M Wakabayashi T Hama (1977) ArticleTitleEffects of histidine-free and -excess diets on anserine and carnosine contents in rat gastrocnemius muscle J Nutr Sci Vitaminol 23 331–340 Occurrence Handle1:CAS:528:DyaE1cXitV2ktg%3D%3D Occurrence Handle915562

  45. S Tomonaga Y Kaji T Tachibana D Denbow M Furuse (2005) ArticleTitleOral administration of β-alanine modifies carnosine concentrations in the muscles and brains of chickens Anim Sci J 76 249–254 Occurrence Handle1:CAS:528:DC%2BD2MXos1ejtLg%3D Occurrence Handle10.1111/j.1740-0929.2005.00263.x

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Harris, R., Tallon, M., Dunnett, M. et al. The absorption of orally supplied β-alanine and its effect on muscle carnosine synthesis in human vastus lateralis. Amino Acids 30, 279–289 (2006). https://doi.org/10.1007/s00726-006-0299-9

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  • Keywords: Carnosine – β-Alanine – Muscle – Buffering – Intracellular pH