Amino Acids

, Volume 31, Issue 3, pp 299–302 | Cite as

Effect of melatonin treatment on oxygen consumption by rat liver mitochondria

  • C. F. Reyes-Toso
  • I. R. Rebagliati
  • C. R. Ricci
  • L. M. Linares
  • L. E. Albornoz
  • D. P. Cardinali
  • A. Zaninovich
Article

Summary.

The objective of this study was to examine the in vivo effect of melatonin on rat mitochondrial liver respiration. Two experiments were performed: For experiment 1, adult male rats received melatonin in the drinking water (16 or 50 µg/ml) or vehicle during 45 days. For experiment 2, rats received melatonin in the drinking water (50 µg/ml) for 45 days, or the same amount for 30 days followed by a 15 day-withdrawal period. At sacrifice, a liver mitochondrial fraction was prepared and oxygen consumption was measured polarographically in the presence of excess concentration of DL-3-β-hydroxybutyrate or L-succinate. Melatonin treatment decreased Krebs’ cycle substrate-induced respiration significantly at both examined doses. The stimulation of mitochondrial respiration caused by excess concentration of substrate recovered after melatonin withdrawal. Basal state 4 respiration was not modified by melatonin. Melatonin, by curtailing overstimulation of cellular respiration caused by excess Krebs’ cycle substrates, can protect the mitochondria from oxidative damage.

Keywords: Melatonin – Oxygen consumption – Free radicals – Liver mitochondria – β-hydroxybutyrate – Succinate 

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • C. F. Reyes-Toso
    • 1
  • I. R. Rebagliati
    • 1
  • C. R. Ricci
    • 1
  • L. M. Linares
    • 1
  • L. E. Albornoz
    • 1
  • D. P. Cardinali
    • 1
  • A. Zaninovich
    • 1
  1. 1.Departamento de Fisiología and Hospital de Clínicas, Facultad de MedicinaUniversidad de Buenos AiresBuenos AiresArgentina

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