Advertisement

Applied Magnetic Resonance

, Volume 45, Issue 12, pp 1343–1358 | Cite as

Spin-label W-band EPR with Seven-Loop–Six-Gap Resonator: Application to Lens Membranes Derived from Eyes of a Single Donor

  • Laxman Mainali
  • Jason W. Sidabras
  • Theodore G. Camenisch
  • Joseph J. Ratke
  • Marija Raguz
  • James S. Hyde
  • Witold K. SubczynskiEmail author
Article

Abstract

Spin-label W-band (94 GHz) electron paramagnetic resonance (EPR) with a five-loop–four-gap resonator (LGR) was successfully applied to study membrane properties (Mainali et al. J Magn Reson 226:35–44, 2013). In that study, samples were equilibrated with the selected gas mixture outside the resonator in a sample volume ~100 times larger than the sensitive volume of the LGR and transferred to the resonator in a quartz capillary. A seven-loop–six-gap W-band resonator has been developed. This resonator permits measurements on aqueous samples of 150 nL volume positioned in a polytetrafluoroethylene (PTFE) gas permeable sample tube. Samples can be promptly deoxygenated or equilibrated with an air/nitrogen mixture inside the resonator, which is significant in saturation-recovery measurements and in spin-label oximetry. This approach was tested for lens lipid membranes derived from lipids extracted from two porcine lenses (single donor). Profiles of membrane fluidity and the oxygen transport parameter were obtained from saturation-recovery EPR using phospholipid analog spin-labels. Cholesterol analog spin-labels allowed discrimination of the cholesterol bilayer domain and acquisition of oxygen transport parameter profiles across this domain. Results were compared with those obtained previously for membranes derived from a pool of 100 lenses. Results demonstrate that EPR at W-band can be successfully used to study aqueous biological samples of small volume under controlled oxygen concentration.

Keywords

Electron Paramagnetic Resonance Electron Paramagnetic Resonance Spectrum Single Donor Lattice Relaxation Time Nitroxide Moiety 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by grants EY015526, EY001931, EB002052, and EB001980 from the National Institutes of Health.

References

  1. 1.
    S.S. Eaton, G.R. Eaton, L.J. Berliner (eds.), Biomedical EPR Part A: Free Radicals, Metals, Medicine, and Physiology, Biological Magnetic Resonance, vol. 23 (Kluwer, Boston, 2005)Google Scholar
  2. 2.
    S.S. Eaton, G.R. Eaton, L.J. Berliner (eds.), Biomedical EPR Part B: Methodology, Instrumentation, and Dynamics, Biological Magnetic Resonance, vol. 24 (Kluwer, Boston, 2005)Google Scholar
  3. 3.
    L.J. Berliner (ed.), Spin Labeling: Theory and Applications (Academic Press, New York, 1976)Google Scholar
  4. 4.
    L.J. Berliner (ed.), Spin Labeling II: Theory and Applications (Academic Press, New York, 1979)Google Scholar
  5. 5.
    Y. Deguchi, J. Chem. Phys. 32, 1584–1585 (1960)ADSCrossRefGoogle Scholar
  6. 6.
    K.H. Hausser, Naturwiss. 47, 251 (1960)ADSCrossRefGoogle Scholar
  7. 7.
    W.Z. Plachy, D.A. Windrem, J. Magn. Reson. 21, 237–239 (1977)ADSGoogle Scholar
  8. 8.
    C.A. Popp, J.S. Hyde, J. Magn. Reson. 43, 249–258 (1981)ADSGoogle Scholar
  9. 9.
    A. Kusumi, W.K. Subczynski, J.S. Hyde, Proc. Natl. Acad. Sci. USA 79, 1854–1858 (1982)ADSCrossRefGoogle Scholar
  10. 10.
    C. Altenbach, D.A. Greenhalgh, H.G. Khorana, W.L. Hubbell, Proc. Natl. Acad. Sci. USA 91, 1667–1671 (1994)ADSCrossRefGoogle Scholar
  11. 11.
    C.S. Klug, J.B. Feix, in Biomedical ESR Part B: Free Radicals, Metals, Medicine, and Physiology, Biological Magnetic Resonance, vol. 24, ed. by S.S. Eaton, G.R. Eaton, L.J. Berliner (Kluwer, Boston, 2005), pp. 269–308Google Scholar
  12. 12.
    W.K. Subczynski, H.M. Swartz, in Biomedical ESR Part A: Free Radicals, Metals, Medicine, and Physiology, Biological Magnetic Resonance, vol. 23, ed. by S.S. Eaton, G.R. Eaton, L.J. Berliner (Kluwer, Boston, 2005), pp. 229–282Google Scholar
  13. 13.
    W. Froncisz, T. Oles, J.S. Hyde, Rev. Sci. Instrum. 57, 1095–1099 (1986)ADSCrossRefGoogle Scholar
  14. 14.
    J.W. Sidabras, R.R. Mett, W. Froncisz, T.G. Camenisch, J.R. Anderson, J.S. Hyde, Rev. Sci. Instrum. 78, 034701-1 (2007)ADSGoogle Scholar
  15. 15.
    J.S. Hyde, J.J. Yin, W.K. Subczynski, T.G. Camenisch, J.J. Ratke, W. Froncisz, J. Phys. Chem. B 108, 9524–9529 (2004)CrossRefGoogle Scholar
  16. 16.
    W.K. Subczynski, L. Mainali, T.G. Camenisch, W. Froncisz, J.S. Hyde, J. Magn. Reson. B 209, 142–148 (2011)ADSCrossRefGoogle Scholar
  17. 17.
    L. Mainali, M. Raguz, T.G. Camenisch, J.S. Hyde, W.K. Subczynski, J. Magn. Reson. 212, 86–94 (2011)ADSCrossRefGoogle Scholar
  18. 18.
    L. Mainali, M. Raguz, W.J. O’Brien, W.K. Subczynski, Biochim. Biophys. Acta 1828, 1432–1440 (2013)CrossRefGoogle Scholar
  19. 19.
    L. Mainali, M. Raguz, W.J. O’Brien, W.K. Subczynski, Exp. Eye Res. 97, 117–129 (2012)CrossRefGoogle Scholar
  20. 20.
    M. Raguz, J. Widomska, J. Dillon, E.R. Gaillard, W.K. Subczynski, Biochim. Biophys. Acta 1788, 2380–2388 (2009)CrossRefGoogle Scholar
  21. 21.
    J. Widomska, M. Raguz, J. Dillon, E.R. Gaillard, W.K. Subczynski, Biochim. Biophys. Acta 1768, 1454–1465 (2007)CrossRefGoogle Scholar
  22. 22.
    M. Raguz, J. Widomska, J. Dillon, E.R. Gaillard, W.K. Subczynski, Biochim. Biophys. Acta 1778, 1079–1090 (2008)CrossRefGoogle Scholar
  23. 23.
    W.K. Subczynski, M. Raguz, J. Widomska, L. Mainali, A. Konovalov, J. Membr. Biol. 245, 51–68 (2012)CrossRefGoogle Scholar
  24. 24.
    M. Raguz, L. Mainali, W.J. O’Brien, W.K. Subczynski, Exp. Eye Res. 120, 138–151 (2014)CrossRefGoogle Scholar
  25. 25.
    L. Mainali, J.S. Hyde, W.K. Subczynski, J. Magn. Reson. 226, 35–44 (2013)ADSCrossRefGoogle Scholar
  26. 26.
    M. Rujoi, J. Jin, D. Borchman, D. Tang, M.C. Yappert, Invest. Ophthalmol. Vis. Sci. 44, 1634–1642 (2003)CrossRefGoogle Scholar
  27. 27.
    R. Estrada, M.C. Yappert, J. Mass Spectrom. 39, 1531–1540 (2004)CrossRefGoogle Scholar
  28. 28.
    J. Folch, M. Lees, G.H. Sloane, Stanley. J. Biol. Chem. 226, 497–509 (1957)Google Scholar
  29. 29.
    J.T. Buboltz, Rev. Sci. Instrum. 80, 124301 (2009)ADSCrossRefGoogle Scholar
  30. 30.
    J. Huang, J.T. Buboltz, G.W. Feigenson, Biochim. Biophys. Acta 1417, 89–100 (1999)CrossRefGoogle Scholar
  31. 31.
    J.T. Buboltz, G.W. Feigenson, Biochim. Biophys. Acta 1417, 232–245 (1999)CrossRefGoogle Scholar
  32. 32.
    R.R. Mett, J.W. Sidabras, J.S. Hyde, Appl. Magn. Reson. 31, 573–589 (2007)CrossRefGoogle Scholar
  33. 33.
    R.R. Mett, J.W. Sidabras, J.S. Hyde, Appl. Magn. Reson. 35, 285–318 (2009)CrossRefGoogle Scholar
  34. 34.
    W. Froncisz, T.G. Camenisch, J.J. Ratke, J.R. Anderson, W.K. Subczynski, R.A. Strangeway, J.W. Sidabras, J.S. Hyde, J. Magn. Reson. 193, 297–304 (2008)ADSCrossRefGoogle Scholar
  35. 35.
    J.S. Hyde, W.K. Subczynski, in Spin Labeling: Theory and Applications, Biological Magnetic Resonance, vol. 8, ed. by L.J. Berliner, J. Reuben (Plenum, New York, 1989), pp. 399–425Google Scholar
  36. 36.
    J.M. Deeley, T.W. Mitchell, X. Wei, J. Korth, J.R. Nealon, S.J. Blanksby, R.J. Truscott, Biochim. Biophys. Acta 1781, 288–298 (2008)CrossRefGoogle Scholar
  37. 37.
    K.A. Earle, D.E. Budil, in Advanced ESR Methods in Polymer Research, ed. by S. Schlick (Wiley, Hoboken, 2006), pp. 54–82Google Scholar
  38. 38.
    E. Meirovitch, J.H. Freed, J. Phys. Chem. 88, 4995–5004 (1984)CrossRefGoogle Scholar
  39. 39.
    L. Mainali, J.B. Feix, J.S. Hyde, W.K. Subczynski, J. Magn. Reson. 212, 418–425 (2011)ADSCrossRefGoogle Scholar
  40. 40.
    W.K. Subczynski, J.S. Hyde, Biophys. J. 41, 283–286 (1983)CrossRefGoogle Scholar
  41. 41.
    J.J. Yin, J.S. Hyde, J. Chem. Phys. 91, 6029–6035 (1989)ADSCrossRefGoogle Scholar
  42. 42.
    W.K. Subczynski, J. Widomska, A. Wisniewska, A. Kusumi, in Lipid Rafts, ed. by T.J. McIntosh. Methods in Molecular Biology, vol. 398 (Humana, Totowa, 2007), pp. 143–157Google Scholar
  43. 43.
    A. Kusumi, W.K. Subczynski, J.S. Hyde, Proc. Natl. Acad. Sci. USA 79, 1854–1858 (1982)ADSCrossRefGoogle Scholar
  44. 44.
    L. Mainali, J.B. Feix, J.S. Hyde, W.K. Subczynski, J. Magn. Reson. 212, 418–425 (2011)ADSCrossRefGoogle Scholar
  45. 45.
    P.F. Devaux, in Biological Magnetic Resonance, vol. 5, ed. by L.J. Berliner, J. Reuben (Plenum, New York, 1983), pp. 183–299Google Scholar
  46. 46.
    S. Schreier, C.F. Polnaszek, I.C. Smith, Biochim. Biophys. Acta 515, 395–436 (1978)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Laxman Mainali
    • 1
  • Jason W. Sidabras
    • 1
  • Theodore G. Camenisch
    • 1
  • Joseph J. Ratke
    • 1
  • Marija Raguz
    • 1
    • 2
  • James S. Hyde
    • 1
  • Witold K. Subczynski
    • 1
    Email author
  1. 1.Department of BiophysicsMedical College of WisconsinMilwaukeeUSA
  2. 2.Department of Medical Physics and Biophysics, School of MedicineUniversity of SplitSplitCroatia

Personalised recommendations