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Applied Magnetic Resonance

, Volume 43, Issue 1–2, pp 207–221 | Cite as

Evaluation of a Shuttle DNP Spectrometer by Calculating the Coupling and Global Enhancement Factors of l-Tryptophan

  • Philip Lottmann
  • Thorsten Marquardsen
  • Alexander Krahn
  • Andreas Tavernier
  • Peter Höfer
  • Marina Bennati
  • Frank EngelkeEmail author
  • Christian GriesingerEmail author
Article

Abstract

A liquid state shuttle dynamic nuclear polarization (DNP) spectrometer is presented, featuring several technical modifications that increase stability and improve reproducibility. For the protons of l-tryptophan, the signal enhancement and the DNP spin properties, such as relaxation, were measured and compared with each other. The calculated coupling factors suggest that the proton accessibility for the polarizer molecule has an important influence on the DNP enhancement. In general, short proton spin longitudinal relaxation times without radical reduce the detectable enhancement by decreasing the leakage factor and increasing the relaxation losses during the course of the sample transfer. The usage of a global enhancement factor gives a more complete overview of the capabilities for the described experimental setup. Global enhancements of up to −4.2 for l-tryptophan protons are found compared to pure Boltzmann enhancements of up to −2.4.

Keywords

Coupling Factor Dynamic Nuclear Polarization Magic Angle Spin Spin Lattice Relaxation Time Relaxation Loss 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We would like to acknowledge M.T. Türke for helpful discussion and T. Michael Sabo for carefully reading the manuscript. This work was supported by the Max Planck Society (to M.B. and C.G.) and by Bio-NMR project 261863 (to C.G. and F.E.).

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Philip Lottmann
    • 1
  • Thorsten Marquardsen
    • 2
  • Alexander Krahn
    • 2
  • Andreas Tavernier
    • 2
  • Peter Höfer
    • 2
  • Marina Bennati
    • 3
  • Frank Engelke
    • 2
    Email author
  • Christian Griesinger
    • 1
    Email author
  1. 1.NMR Based Structural BiologyMax Planck Institute for Biophysical ChemistryGöttingenGermany
  2. 2.Bruker BioSpinRheinstettenGermany
  3. 3.Electron Paramagnetic ResonanceMax Planck Institute for Biophysical ChemistryGöttingenGermany

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