Spektrum der Augenheilkunde

, Volume 24, Issue 6, pp 305–310 | Cite as

Apoptotic death ligands and interleukins in the vitreous of diabetic patients

  • R. Maier
  • M. Weger
  • E.-M. Haller-Schober
  • B. Huppertz
  • L. M. Maier
  • Y. El-Shabrawi
  • A. Wedrich
  • A. Theisl
  • W. Graninger
  • U. Demel
  • A. Haas
Original article
  • 39 Downloads

Summary

BACKGROUND: To investigate the role of apoptosis in the pathogenesis of diabetic retinopathy we determined vitreous concentrations of Fas ligand (FasL), tumor necrosis factor alpha (TNF-α), lymphotoxin alpha (LT-α) and interleukin 1beta (IL-1β) in diabetic patients and non-diabetic control subjects. MATERIAL AND METHODS: In the present case-control study measurement of vitreous concentrations of the four apoptosis-related factors FasL, TNF-α, LT-α and IL-1β was performed using Cytometric Bead Array technology in 48 patients with diabetes mellitus (8 non-insulin dependent, 40 insulin dependent) and 24 non-diabetic control subjects. RESULTS: FasL, TNF-α, LT-α and IL-1β were not detectable in the vitreous of neither diabetic nor non-diabetic controls samples. CONCLUSION: This study suggests that there is no up-regulation of FasL, TNF-α, LT-α and IL-1β in the vitreous of diabetic patients.

Keywords

Diabetes Retina Apoptosis Vitreous Interleukin 

Apoptotische Liganden und Interleukine im Glaskörper von Patienten mit Diabetes mellitus

Zusammenfassung

HINTERGRUND: Um den Einfluss der Apoptose in der Pathogenese der diabetischen Retinopathie zu untersuchen, wurden Glaskörper-Konzentrationen von Fas ligand (FasL), tumor necrosis factor alpha (TNF-α), lymphotoxin alpha (LT-α) and interleukin 1beta (IL-1β) bei Diabetikern und Nicht-Diabetikern bestimmt. MATERIAL UND METHODEN: In der vorliegenden Fall-Kontroll-Studie wurde die Messung der Glaskörper-Konzentrationen der Apoptose-Faktoren FasL, TNF-α, LT-α and IL-1β unter Verwendung Cytometric Bead Array-Technologie bei 48 Patienten mit Diabetes mellitus (8 nicht-Insulin-abhängigen, 40 insulinpflichtig) und 24 nicht-diabetischen Kontrollpersonen vorgenommen. ERGEBNISSE: Im Glaskörper waren die apoptotischen Faktoren FasL, TNF-α, LT-α and IL-1β weder in den diabetischen Patienten noch in den nicht-diabetischen Kontrollen nachweisbar. SCHLUSSFOLGERUNG: In dieser Studie konnte keine Hochregulation FasL, TNF-α, LT-α and IL-1β im Glaskörper von diabetischen Patienten nachgewiesen werden.

Schlüsselwörter

Diabetes mellitus Netzhaut Apoptose Glaskörper Interleukin 

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • R. Maier
    • 1
  • M. Weger
    • 1
  • E.-M. Haller-Schober
    • 1
  • B. Huppertz
    • 2
  • L. M. Maier
    • 4
  • Y. El-Shabrawi
    • 1
  • A. Wedrich
    • 1
  • A. Theisl
    • 1
  • W. Graninger
    • 3
  • U. Demel
    • 3
  • A. Haas
    • 1
  1. 1.Department of OphthalmologyMedical University of GrazGrazAustria
  2. 2.Institute of Cell Biology, Histology and EmbryologyMedical University of GrazGrazAustria
  3. 3.Division of RheumatologyMedical University GrazGrazAustria
  4. 4.Center for Neurologic Diseases, Brigham and Women's HospitalHarvard Medical SchoolBoston, MAUSA

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