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Protoplasma

pp 1–9 | Cite as

Appraisal of immediate and late effects of mobile phone radiations at 2100 MHz on mitotic activity and DNA integrity in root meristems of Allium cepa

  • Shikha Chandel
  • Shalinder KaurEmail author
  • Mohd Issa
  • Harminder Pal Singh
  • Daizy Rani Batish
  • Ravinder Kumar Kohli
Original Article

Abstract

The present study evaluated the potential of 2100 MHz radiofrequency radiations to act as cytotoxic and genotoxic agent. Fresh onion (Allium cepa L.) roots were exposed to electromagnetic field radiations (EMF-r) for different durations (1 h and 4 h) and evaluated for mitotic index (MI), phase index, chromosomal aberrations, and DNA damage. DNA damage was investigated with the help of the comet assay by assessing various parameters like % head DNA (HDNA), % tail DNA (TDNA), tail moment (TM), and olive tail moment (OTM). Effects of EMF-r exposure were also compared with that of methyl methanesulfonate (MMS; 90 μM), which acted as a positive control. The post-exposure effects of EMF-r after providing the test plants with an acclimatization period of 24 h were also evaluated. Compared to the control, a significant increase in the MI and aberration percentage was recorded upon 4 h of exposure. However, no specific trend of phase index in response to exposure was detected. EMF-r exposure incited DNA damage with a significant decrease in HDNA accompanied by an increase in TDNA upon exposure of 4 h. However, TM and OTM did not change significantly upon exposure as compared to that of control. Analysis of the post-exposure effects of EMF-r did not show any significant change/recovery. Our data, thus, suggest the potential cytotoxic and genotoxic nature of 2100 MHz EMF-r. Our study bears great significance in view of the swiftly emergent EMF-r in the surrounding environment and their potential for inciting aberrations at the chromosomal level, thus posing a genetic hazard.

Keywords

Electromagnetic field radiations Onion Chromosomal aberrations Genotoxicity Recovery 

Notes

Acknowledgments

The authors are thankful to the Science and Engineering Research Board, Department of Science and Technology, New Delhi (India) for financial support.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Shikha Chandel
    • 1
  • Shalinder Kaur
    • 1
    Email author
  • Mohd Issa
    • 2
  • Harminder Pal Singh
    • 2
  • Daizy Rani Batish
    • 1
  • Ravinder Kumar Kohli
    • 1
    • 3
  1. 1.Department of BotanyPanjab UniversityChandigarhIndia
  2. 2.Department of Environment StudiesPanjab UniversityChandigarhIndia
  3. 3.Central University of PunjabBathindaIndia

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