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Protoplasma

, Volume 256, Issue 5, pp 1361–1373 | Cite as

Centrosome-derived microtubule radial array, PCM-1 protein, and primary cilia formation

  • I. Fokin Artem
  • N. Zhapparova Olga
  • V. Burakov Anton
  • S. Nadezhdina ElenaEmail author
Original Article

Abstract

In animal cells, the centrosome nucleates and anchors microtubules (MT), forming their radial array. During interphase centrosome-derived MT, aster can either team up with other MT network or function in an autonomous manner. What is the function of the centrosome-derived MT aster? We suggested that it might play an important role in the formation of the primary cilium, the organelle obligatorily associated with the centrosome. PCM-1 (PeriCentriolar Matrix 1) protein, which participates in the organization of the primary cilium, is a part of pericentiolar satellites. They are transported to the centrosome along MTs by the motor protein dynein in a complex with its cofactor dynactin. Previously, we showed that SLK/LOSK phosphorylated the p150Glued subunit of dynactin, thus promoting its centrosomal targeting followed by its participation in the retention of microtubules. Here, we found that under the repression of SLK/LOSK activity, the PCM-1 protein lost its pericentrosomal localization and was being dispersed throughout the cytoplasm. Despite that the alanine and glutamine mutants of p150Glued had opposite effects on PCM-1 localization, they associated with PCM-1 to the same extent. The occurrence of primary cilia also significantly decreased when SLK/LOSK was repressed. These defects also correlated with a disturbance of the long-range transport in cells, whereas dynein-depending motility was intact. Treatment with the GSK-3β kinase inhibitor also resulted in the loss of the centrosome-derived MT aster, dispersion of PCM-1 over the cytoplasm, and reduction of primary cilia occurrence. Thus, kinases involved in the centrosome-derived MT aster regulation can indirectly control the formation of primary cilia in cells.

Keywords

Protein kinase Dynactin GSK-3β SLK LOSK Dynein-driven transport PCM-1 Primary cilium 

Notes

Acknowledgments

The authors are very grateful to Prof. Andrea Merdes (Université Paul Sabatier/CNRS, Toulouse) for the great anti-PCM-1 antibody. We also thank Dr. Hans-Peter Hauri (University of Basel) for DNA construct, Dr. Ilya Brodsky (Moscow State University) for DNA constructs and fruitful discussions, and Ekaterina Ryabkova for technical assistance.

Funding information

The project was financially supported by the President of Russain Federation grant for young PhD researchers (MK-8703.2010.4) and grants from the Russian Basic Research Foundation (08-04-01697-a, 11-04-01022-a, and 18-04-00742-a).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.A.N. Belozersky Institute for Physico-Chemical BiologyM.V. Lomonosov Moscow State UniversityMoscowRussian Federation
  2. 2.Department of Cell Biology of Institute of Protein ResearchRussian Academy of ScienceMoscowRussian Federation

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