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Protoplasma

pp 1–7 | Cite as

In situ assessment of mitochondrial calcium transport in tobacco pollen tubes

  • Cesar Flores-Herrera
  • Gisela Preciado-Linares
  • Israel Gonzalez-Vizueth
  • Norma Corona de la Peña
  • Manuel Gutiérrez-Aguilar
Original Article
  • 111 Downloads

Abstract

Pollen tubes require functional mitochondria in order to achieve fast and sustained growth. In addition, cell wall expansion requires a calcium gradient in the tube apex formed by a dedicated array of calcium pumps and channels. Most studies have traditionally focused on the molecular aspects of calcium interactions and transport across the pollen tube plasmalemma. However, calcium transients across mitochondrial membranes from pollen tubes are beginning to be studied. Here, we report the presence of a ruthenium red-sensitive mitochondrial calcium uniporter-like activity in tobacco pollen tubes with functional oxidative phosphorylation. The present study provides a framework to measure in situ specifics of mitochondrial transport and respiration in pollen tubes from different plants. The relevance of a mitochondrial calcium uniporter for pollen tube growth is discussed.

Keywords

Pollen tubes Nicotiana Mitochondrial calcium uniporter Transport Digitonin 

Abbreviations

MCU

mitochondrial calcium uniporter

AtMCU1

Arabidopsis thaliana mitochondrial calcium uniporter 1

AtMCU2

Arabidopsis thaliana mitochondrial calcium uniporter 2

MICU

EF-hand MCU regulator

MPT

mitochondrial permeability transition

PG

pollen germination

PTA

pollen tube assay

RuR

ruthenium red

ROS

reactive oxygen species

Notes

Acknowledgements

This work was supported by grant UNAM-FQ-PAIP 5000-9171 (to M.G.-A.). We would like to thank Dr. Sobeida Sánchez-Nieto and Dr. Felipe Cruz-García for providing valuable material resources and advice for the completion of this study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

709_2018_1316_Fig5_ESM.png (509 kb)
Figure S1

(A) Predicted Nicotiana tabacum MCU orthologues using Mus musculus MCU as template were found by a Basic Local Alignment Search Tool and aligned using Clustal Omega. Highly conserved residues encompassing the RuR- and calcium-binding domain are colored. Mitochondrial import probability (P) was assessed using Mitoprot and displayed after each predicted protein sequence segment alignment. (B) Mus musculus MICU1 was used as template for a Basic Local Alignment Search Tool. A phylogenetic tree was constructed using with the NCBI phylogenetic tree tool. (PNG 509 kb)

709_2018_1316_MOESM1_ESM.tif (1.1 mb)
High Resolution Image (TIF 1163 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Departamento de Bioquímica, Facultad de QuímicaUniversidad Nacional Autónoma de MéxicoMéxico CityMexico
  2. 2.Unidad de Investigación en Trombosis, Hemostasia y AterogénesisHospital Carlos McGregorMéxico CityMexico

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