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Protoplasma

, Volume 256, Issue 1, pp 193–211 | Cite as

Identification of arsenic-tolerant and arsenic-sensitive rice (Oryza sativa L.) cultivars on the basis of arsenic accumulation assisted stress perception, morpho-biochemical responses, and alteration in genomic template stability

  • Barsha Majumder
  • Susmita Das
  • Sandip Mukhopadhyay
  • Asok K. BiswasEmail author
Original Article
  • 160 Downloads

Abstract

Arsenic toxicity is the most commonly experienced challenge of rice plants due to irrigation with arsenic-polluted groundwater and their cultivation in water logging environment which poses threat to human health, particularly in Bangladesh and West Bengal (India). In the present study, hydroponically grown eight rice cultivars, viz., Bhutmuri, Kumargore, Binni, Vijaya, Tulsibhog, Badshabhog, Pusa basmati, and Swarnadhan, were screened for arsenic tolerance by using physiological and molecular parameters. Treatment with 25 μM, 50 μM, and 75 μM arsenate resulted in dosage-based retardation in growth and water content in all the tested cultivars due to accumulation of total arsenic along with the enhanced activity of arsenate reductase with more severe effects exhibited in cvs. Swarnadhan, Pusa basmati, Badshabhog, and Tulsibhog. Arsenic sensitivity of rice cultivars was evaluated in terms of oxidative stress markers generation, antioxidant enzyme activities, and level of genotoxicity. Under arsenate-challenged conditions, the levels of oxidative stress markers, viz., H2O2, MDA, and proline, and activities of antioxidant enzymes, viz., SOD and CAT, along with the level of genotoxicity analyzed by RAPD profiling were altered in variable levels in all tested rice cultivars and showed a significant alteration in band patterns in arsenate-treated seedlings of cvs. Swarnadhan, Pusa basmati, Badshabhog, and Tulsibhog in terms of appearance of new bands and disappearance of normal bands that were presented in untreated seedlings led to reduction in genomic template stability due to their high susceptibility to arsenic toxicity. Cultivar- and dose-dependent alteration of parameters tested including the rate of As accumulation showed that cvs. Kumargore, Binni, and Vijaya, specially Bhutmuri, were characterized as arsenate tolerant and could be cultivated in arsenic-prone areas to minimize level of toxicity and potential health hazards.

Keywords

Arsenate Rice cultivar Screening Oxidative stress RAPD GTS 

Abbreviations

ANOVA

One-way variance

As

Arsenic

AsIII

Arsenite

AsV

Arsenate

CAT

Catalase

DNA

Deoxyribonucleic acid

DW

Dry weight

FW

Fresh weight

EU

Enzyme unit

GTS

Genomic template stability

H2O2

Hydrogen peroxide

MDA

Malondialdehyde

mM

Millimolar

μM

Micromole

RAPD

Random amplified polymorphic DNA

ROS

Reactive oxygen species

SOD

Superoxide dismutase

Notes

Acknowledgements

The authors are grateful to Dr. Baidya Nath Pal, Associate Scientist ‘A’ of Indian Statistical Institute, Kolkata for his valuable guidance during enormous statistical analysis. The authors also acknowledge the facilities, provided by Centre of Advanced Study, Department of Botany (CAS Phase VI, VII), University of Calcutta, and Scientific Research Laboratory, Santoshpur, Kolkata.

Funding information

The study was financially supported by University Grants Commission (UGC) funded Major Research Project (MRP), New Delhi (F.No-43-102/2014 (SR), dt 18.01.2016).

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Barsha Majumder
    • 1
    • 2
  • Susmita Das
    • 1
  • Sandip Mukhopadhyay
    • 2
  • Asok K. Biswas
    • 1
    Email author
  1. 1.Plant Physiology & Biochemistry Laboratory, Centre of Advanced Study, Department of BotanyUniversity of CalcuttaKolkataIndia
  2. 2.Molecular Biology & Plant Biotechnology Laboratory, Centre of Advanced Study, Department of BotanyUniversity of CalcuttaKolkataIndia

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