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Protoplasma

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Cytological differentiation and cell wall involvement in the growth mechanisms of articulated laticifers in Tabernaemontana catharinensis A.DC. (Apocynaceae)

  • Yve Canaveze
  • Alexandra Antunes Mastroberti
  • Jorge Ernesto de Araujo Mariath
  • Silvia Rodrigues Machado
Original Article
  • 228 Downloads

Abstract

The cellular mechanisms of laticifer growth are of particular interest in plant biology but are commonly neglected. Using transmission electron microscopy and immunocytochemical methods, we recorded cytological differentiation and evaluated the cell wall involvement in the growth of articulated laticifers with intrusive growth in the mature embryo and plant shoot apex of Tabernaemontana catharinensis. The incorporation of adjacent meristematic cells into the laticifer system occurred in the embryo and plant shoot apex, and the incorporated cells acquired features of laticifer, confirming the laticifers’ action-inducing mechanism. In the embryo, this was the main growth mechanism, and began with enlargement of the plasmodesmata and the formation of pores between laticifers and meristematic cells. In the plant shoot apex, it began with loose and disassembled walls and the reorientation of the cortical microtubules of the incorporated cell. Plasmodesmata were absent in these laticifers. There was stronger evidence of intrusive growth in undifferentiated portions of the plant shoot apex than in the embryo. The numerous plasmodesmata in laticifers of the embryo may have been related to the lower frequency of intrusive growth. Intrusive growth was associated with presence of arabinan (increasing wall flexibility and fluidity), and absence of galactan (avoiding wall stiffness), and callose (as a consequence of reduction in symplastic connections) in the laticifer walls. The abundance of low de-methyl-esterified homogalacturonan in the middle lamella and corners may reestablish cell-cell bonding in the laticifers. The cell wall features differed between embryo and plant shoot apex and are directly associated to laticifer growth mechanisms.

Keywords

Immunocytochemistry Intrusive growth Laticifer system Laticifers’ action-inducing mechanism Ultrastructure 

Notes

Acknowledgements

Thanks to the Electron Microscopy Centre (CME) IBB, UNESP, and its technicians for assistance with sample preparations. We are also thankful to Prof. J.-P Knox (University of Leeds, Centre of Plant Science, UK) for monoclonal antibodies against HG and RG-I kindly provided to the Laboratory of Plant Anatomy, UFRGS.

Funding information

This study is financially supported by the National Council for Scientific and Technological Development (CNPq - financial support Proc. 473289/2010 and grants to the SR Machado Proc. 302657/2011-8) and the São Paulo Council for Research (FAPESP - financial support Thematic Project Proc. 2008/55434-7 and grants to the first author - Proc. 2012/16441-3).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Yve Canaveze
    • 1
  • Alexandra Antunes Mastroberti
    • 2
  • Jorge Ernesto de Araujo Mariath
    • 2
  • Silvia Rodrigues Machado
    • 1
  1. 1.Department of Botany, Institute of BiosciencesUniversidade Estadual Paulista – UNESPBotucatuBrazil
  2. 2.Laboratory of Plant Anatomy, Department of BotanyUniversidade Federal do Rio Grande do SulPorto AlegreBrazil

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