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Cold plasma treatment and exogenous salicylic acid priming enhances salinity tolerance of Oryza sativa seedlings

  • Mohamed S. Sheteiwy
  • Jianyu An
  • Mengqi Yin
  • Xiaowen Jia
  • Yajing Guan
  • Fei He
  • Jin Hu
Original Article
  • 123 Downloads

Abstract

The present study was designed to highlight the effects of cold plasma (10 kV) treatment and priming with 2 mM salicylic acid (SA) and their combination (10 kV of plasma + 2 mM SA) on the physiological parameters and metabolism of two cultivars of Oryza sativa, i.e., Zhu Liang You 06 (ZY) and Qian You No. 1 (QY), under salinity stress (150 mM NaCl) and normal growth condition (0 mM NaCl). Seed germination and seedling growth were enhanced by SA priming and cold plasma treatment either alone or in combination under salinity stress. Photosynthetic pigments, photosynthetic gas exchange, and chlorophyll fluorescence were improved by cold plasma treatment and SA priming under salinity stress as compared to the untreated seeds. The activities of antioxidant enzymes were significantly improved by the combination of SA priming and cold plasma treatment in both cultivars under salinity stress. There were rapid changes in the cellular content of sodium (Na+) and calcium (Ca+), where the plants grown under saline conditions accumulate more Na+ and less Ca+ contents resulting in ionic imbalances. Interestingly, cold plasma and SA treatments diminished this action by reducing Na+ accumulation and increasing K+ and Ca+ contents in the plant cell under salinity stress. The activities of enzymes involved in secondary metabolism assimilation were up-regulated with cold plasma and SA priming either alone or combination under salinity stress. An increase in reactive oxygen species (ROS) accumulation and malondialdehyde (MDA) content was also observed under salinity stress condition. On contrast, seed treated with SA and plasma alone or combined resulted in a significant decrease in ROS and MDA contents under salinity stress. Our results indicated that SA priming and cold plasma treatment either alone or combined improved plant uptake of nutrients in both cultivars under stress conditions. The ultrastructural changes were observed to be more prominent in ZY than QY cultivar. Plants without SA priming or cold plasma treatments have a big vacuole due to the movement of ions into the vacuole directly from the apoplast into the vacuole through membrane vesiculation leading to membrane destabilization. However, SA priming and cold plasma treatment alone or combined helped the plants to recover their cell turgidity under salinity stress.

Keywords

Salinity SA Cold plasma Oryza sativa Antioxidant Ultrastructure Secondary metabolism 

Notes

Acknowledgments

This research was supported by the Fund of National Key Research and Development Project (No. 2018YFD0100902), the National Natural Science Fund (no. 31671774 and 31371708), Zhejiang Provincial Natural Science Foundation (LZ14C130002, LY15C130002), the Project of the Science and Technology Department of Zhejiang Province (no. 2013C02005), and Jiangsu Collaborative Innovation Center for Modern Crop Production, P.R. China.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

709_2018_1279_MOESM1_ESM.docx (317 kb)
ESM 1 (DOCX 316 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018
corrected publication [July/2018]

Authors and Affiliations

  1. 1.Seed Science Center, College of Agriculture and BiotechnologyZhejiang UniversityHangzhouChina
  2. 2.Department of Agronomy, Faculty of AgricultureMansoura UniversityMansouraEgypt

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