The terrestrial green macroalga Prasiola calophylla (Trebouxiophyceae, Chlorophyta): ecophysiological performance under water-limiting conditions
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The phylogenetic placement of Prasiola calophylla, from an anthropogenic habitat previously shown to contain a novel UV sunscreen compound, was confirmed by analysis of its rbcL gene. This alga has the capacity to tolerate strong water-limiting conditions. The photosynthetic performance and ultrastructural changes under desiccation and osmotic stress were investigated. Freshly harvested thalli showed an effective quantum yield of PSII [Y(II)] of 0.52 ± 0.06 that decreased to ∼60% of the initial value at 3000 mM sorbitol, and 4000 mM sorbitol led to a complete loss of Y(II). The Y(II) of thalli exposed to controlled desiccating conditions at 60% relative humidity (RH) ceased within 240 min, whereas zero values were reached after 120 min at 20% RH. All investigated samples completely recovered Y(II) within ∼100 min after rehydration. Relative electron transport rates (rETR) were temperature dependent, increasing from 5, 10, to 25 °C but strongly declining at 45 °C. Transmission electron microscopy of samples desiccated for 2.5 h showed an electron dense appearance of the entire cytoplasm when compared to control samples. Thylakoid membranes were still visible in desiccated cells, corroborating the ability to recover. Control and desiccated cells contained numerous storage lipids and starch grains, providing reserves. Overall, P. calophylla showed a high capacity to cope with water-limiting conditions on a physiological and structural basis. A lipophilic outer layer of the cell walls might contribute to reduce water evaporation in this poikilohydric organism.
KeywordsChlorophyll fluorescence Desiccation Light Photosynthesis Temperature
Mycosporine-like amino acid
Some green algae in the class Trebouxiophyceae (Chlorophyta), particularly members of the genus Prasiola, show a cosmopolitan distribution (Rindi 2007; Moniz et al. 2012; Heesch et al. 2016; Richter et al. 2016). The ecology of Prasiola is diverse, with some species growing in freshwater ecosystems or the supralittoral zone of marine coasts, and others occurring in terrestrial habitats (Friedmann 1969; Rodriguez et al. 2007; Rindi and Guiry 2004). Among terrestrial members, species such as Prasiola crispa ssp. antarctica even exhibit a symbiotic lifestyle with Ascomycota, forming the lichen Mastodia tessellata (Pérez-Ortega et al. 2010). In polar regions, Prasiola species prefer habitats rich in nitrogen and are found with faeces of birds such as penguins and growing in association with seagull colonies (Jackson and Seppelt 1997; Lud et al. 2001; Holzinger et al. 2006; Richter et al. 2016). Terrestrial Prasiola species are exposed to extremely harsh abiotic conditions, including strong water potential gradients between the soil surface and the surrounding atmosphere, resulting in regular desiccation that reduces the net production phases (Holzinger and Karsten 2013; Karsten and Holzinger 2014). In addition, terrestrial algae experience strong diurnal and seasonal fluctuations in insolation, including ultraviolet radiation (UVR). As a photoprotective mechanism under enhanced UVR, Prasiola species and related trebouxiophycean taxa synthesize and accumulate a natural sunscreen, a mycosporine-like amino acid (MAA) (e.g. Karsten et al. 2005). This compound, recently characterized from Prasiola calophylla, has a novel chemical structure composed of an oxo-carbonyl MAA (Hartmann et al. 2016). Given that prasiolin was described in P. calophylla, a detailed examination of the accompanying ecophysiology and cell structure/ultrastructure of this species during desiccation is needed.
The habitat occupied by Prasiola is an example of an ecological boundary between atmosphere and surface, in this particular case, a concrete wall covered by an extremely thin layer of soil. Boundaries tend to take on emergent properties that neither bordering region possess and will tend to modulate transfer of molecules and energy from one side to the other (Strayer et al. 2003). Many physiological activities and functions of terrestrial algae are related to trapping, transmission, transformation and/or loss of molecules across the atmosphere-soil interface that is occupied by these organisms. Water uptake and water loss represent key processes for the physiological activity of terrestrial algae.
Desiccation-tolerant terrestrial algae such as P. crispa ssp. antarctica often exhibit morphological adaptations, including thick cell walls that protect the protoplasts from mechanical damage and the capability to synthesize and accumulate sugar alcohols as ‘water-keeping’ substances (Jacob et al. 1991, 1992). Any damage due to desiccation limits the distribution of these species, and they usually grow in areas where at least occasional moisture is available. Damage caused by water-limiting conditions has recently been extensively reviewed for green algae (Karsten and Holzinger 2014; Holzinger and Pichrtová 2016). Fernandez-Marin et al. (2016) give a particular focus on the different photosynthetic mechanisms of desiccation-tolerant plant species.
The present study focused on the photophysiological and ultrastructural response of P. calophylla to naturally occurring water limitations in an extreme terrestrial habitat. Specifically, we investigated the (1) photosynthetic performance in response to cellular water loss (desiccation or osmotic stress) and (2) temperature dependence of photosynthesis, since the natural habitat has strong diurnally temperature changes due to varying sun exposures. Finally, we examined (3) the structural and ultrastructural features that might be responsible for the observed wide ecological tolerance. As this species is a promising model system for the physiological mechanisms of a terrestrial lifestyle, we also aimed to confirm its phylogenetic position by rbcL marker analyses. An unambiguous molecular taxonomic assignment is crucial, particularly in Prasiola, where morphological variation often occurs (Moniz et al. 2012; Heesch et al. 2016).
Materials and methods
Prasiola calophylla (Carmichael ex Greville) Kützing (Trebouxiophyceae, Chlorophyta) was isolated repeatedly from the Botanical Garden of the University of Innsbruck (611 m above sea level, 47° 16′ 2″ N, 11° 23′ 34″ E) in July 2014, July 2015 and July 2016, as described by Hartmann et al. (2016) and either transferred to Storrs, Connecticut, USA, as life sample for DNA extraction, or immediately used for photophysiological, microscopic and transmission electron microscopic (TEM) examinations as described in the following sections. A voucher specimen of the sample used for DNA extraction was deposited at the George Safford Torrey Herbarium at the University of Connecticut (CONN accession no. 273714, barcode CONN00209659).
DNA sequencing and phylogenetic analysis
Fresh P. calophylla material was extracted using the PowerPlant DNA Isolation Kit (MO BIO Laboratories, Inc., Carlsbad, CA, USA) according to the manufacturer’s protocol. Two primer sets were used for amplification and sequencing of the rbcL region, the PF2/PR2 primer pair (Rindi et al. 2004) and the SHF5/SHR8 pair (Heesch et al. 2012). The PCR mix consisted of 2 μL of extracted DNA, 10 μL of GoTaq® Green Master Mix, 0.5 μL of each primer (10 μM) and sterile deionized water for a final volume of 20 μL in the PCR tubes. The amplification cycle consisted of an initial denaturation step at 95 °C for 4 min, followed by 30 cycles of denaturation at 95 °C for 30 s, annealing at 47 or 50 °C for 30 s and extension at 72 °C for 1 min, with a final extension at 72 °C for 7 min. The sequencing reactions were performed using ABI PRISM BigDye Terminator Cycle Sequencing Kits and run on an ABI PRISM 3730XL Analyzer (Applied Biosystems). The sequences were verified in Geneious 9.0.5 (Biomatters, Auckland, New Zealand) and then used to prepare a consensus, which was subsequently aligned manually with the published rbcL sequences of related taxa. The appropriate substitution model was chosen using Modeltest in PAUP* 4.0a149 (Swofford 2002) using the Akaike information criterion (AICc). The best model selected under AIC was the general time reversible (GTR) + I + gamma. The model parameter values were set during the search based on a pilot analysis: RA-C = 1.0000, RA-G = 4.43327, RA-T = 1.0000, RC-G = 1.4440773, RC-T = 8.23139, RG-T = 1.0000, pinvar = 0.711319 and gamma shape = 0.937307 (four rate categories). A heuristic search with 100 random additions was performed to produce a ML tree in PAUP* using tree bisection-reconnection (TBR) branch swapping. Bootstraps analysis was performed using the same model parameters, with 100 replicates. Parsimony bootstrap analysis (1000 replicates) was performed, also in PAUP*, with TBR branch swapping. MrBayes 3.2.1 was used for the Bayesian analysis using the GTR + I + gamma model (Huelsenbeck and Ronquist 2001; Ronquist and Huelsenbeck 2003). Two independent Bayesian analyses were run for 5.1 × 106 generations with one cold plus three heated chains, with a subsample frequency of 1000. Convergence was determined, and trees from the initial 105 generations were discarded as burn in before producing the majority-rule consensus tree.
Relative electron transport rates and temperature dependence
Relative electron transport rates (rETR) in response to increasing photosynthetically active radiation (PAR) up to ∼1600 μmol photons m−2 s−1 (PI curve) were monitored with a PAM-2500 chlorophyll fluorometer (Heinz Walz GmbH, Effeltrich, Germany) as previously described (Karsten et al. 2014; Herburger et al. 2015). Fresh algal material was harvested on a sunny day (July 2015) and PI curves (n = 4) were recorded at four different temperatures (5, 10, 20, 25 and 45 °C) in a KS-2500 suspension cuvette (Heinz Walz GmbH).
rETR curves were mathematically fitted with the photosynthesis model of Walsby (1997) to derive the alpha value (α; slope of the curve under light-limiting conditions), initial light saturation point (I k; μmol photons m−2 s−1) and maximum rETR (rETRmax).
Desiccation experiment and osmotic stress treatments
Desiccation experiments were performed in a desiccation chamber as previously described by Karsten et al. (2014), with modifications. Prasiola calophylla was either collected on a sunny dry summer day (July 2015) and exposed to 20 or 60% relative air humidity (RH) inside the chamber or collected on a rainy day during the same season and exposed to 20% RH. To generate water-limiting conditions via an extremely negative water potential, the samples were incubated in increasing concentrations of sorbitol (500, 1000, 2000, 3000 or 4000 mM) for 2 h according to the well-established approach of Kaplan et al. (2013) and references therein.
Confocal and transmission electron microscopy
Confocal laser scanning microscopy (CLSM) was performed by using a Zeiss Pascal system (Carl Zeiss AG, Jena, Germany) on a Zeiss Axiovert 200 M. Chloroplast autofluorescence was visualized by exciting either fresh or desiccated thalli for 2.5 h (20% RH) at 488 nm and collecting emission above 560 nm (long pass filter, false color red). Corresponding bright field images were merged with autofluorescence images. Transmission electron microscopy was essentially performed as previously described (Holzinger et al. 2006). Freshly harvested samples were collected on a rainy day, mainly to illustrate the naturally fully hydrated condition, and either fixed directly or experimentally desiccated over a silica gel for 2.5 h prior to fixation. Briefly, samples were fixed in 2.5% glutaraldehyde in 50 mM cacodylate buffer for 1.5 h, rinsed and postfixed overnight in 1% OsO4 in the same buffer at 4 °C. Samples were then dehydrated in increasing ethanol concentrations, embedded in modified Spurr’s low viscosity resin sectioned and poststained with 2% uranyl acetate and Reynold’s lead citrate. Samples were viewed at a Zeiss Libra transmission electron microscope at 80 kV. Images were generated with a ProScan 2 k SSCCD camera and further processed with the Adobe Photoshop Elements 11 software (Adobe Systems, San José, CA, USA).
Nile red staining
Freshly harvested P. calophylla thalli were washed in phosphate-buffered saline (PBS, 100 mM, pH = 7) and transferred to fresh PBS containing 0.1% (v/v) nile red (Sigma-Aldrich, Steinheim, Germany), which was previously dissolved in acetone (0.25% (w/v)). Staining was performed at an RT for 30 min. Thalli were washed 2× with PBS and investigated with a Zeiss Axiovert 200M equipped with an OSRAM HBO 50 Q/AC L1 CZ Mercury short ARC Photo optic lamp and Zeiss filter set 15 (excitation: BP 546/12 nm; emission: LP 590 nm).
Statistical evaluation of the physiological data
Photosynthetic parameters derived from rETR curves at different temperatures (α, I k, rETRmax; n = 4; p < 0.05), Y(II) values (n = 4; p < 0.01) under different sorbitol concentrations and Y(II) values (n = 5; p < 0.05) in response to desiccation/rehydration treatment were compared by one-way ANOVA followed by Tukey’s post hoc test. Data were analysed by Origin 8.5 (OriginLab Corporation, Northampton, MA, USA).
Temperature-dependent photosynthetic performance
Desiccation and rehydration kinetics
Water potential effects
Light and confocal microscopic observations
Transmission electron microscopy of control and desiccated cells
In the present study, the photophysiological performance of P. calophylla was investigated in response to water-limiting conditions as well as ultrastructural changes upon severe desiccation. Moreover, a phylogenetic characterization was performed based on the rbcL gene analysis, confirming the previous morphological taxonomic characterization (Hartmann et al. 2016). This species is especially interesting ecologically, as an abundant occurrence of a new 324-nm absorbing UV sunscreen—termed ‘prasiolin’—was isolated and chemically characterized in natural samples from the same locality (Hartmann et al. 2016). This photoprotectant likely contributes to the ecological success of P. calophylla in aeroterrestrial habitats.
Ephemeral Prasiola species have the capability to grow outside the aquatic milieu on bark, soil and rock and in the supralittoral zone of marine rocky shores (Rindi et al. 2007). This broad ecological amplitude makes this genus particularly interesting to study physiological adaptations to terrestrial habitats, which base either one a freshwater or a marine lifestyle. As far as known, the terrestrial members of this genus always seem to prefer nitrophilic environments shaped by bird faeces, as reported for Antarctic and Arctic taxa (Holzinger et al. 2006; Kosugi et al. 2010; Richter et al. 2016). This is confirmed for P. calophylla of the present study, which benefits from dog excrements (Hartmann et al. 2016). Nitrogen is essential not only for growth but also for the biosynthesis of the N-containing UV sunscreen prasiolin. A similar nitrogen dependence for the production of UV-protecting substances has been shown in the red alga Porphyra (Korbee et al. 2005).
Phylogenetic position and morphological characteristics
Kützing (1848) originally described P. calophylla (Carmichael ex Greville) Kützing from Argyll, Scotland, as dark green, curly and almost linear or ribbon shaped, with filaments gradually narrowing toward the base (Greville 1826). The species is found in cold temperate regions of the Northern Hemisphere (Guiry and Guiry 2016) and was recently reported from a wharf in Japan (Sutherland et al. 2016). Several Antarctic records of this species exist; however, Moniz et al. (2012) indicated that the rbcL sequences of these specimens diverged by 38–42 bp from European specimens of P. calophylla, far greater than the variation even between the accessions from northern Europe and Japan. Moreover, a significantly different habitat (stream- and water-flushed ground distant from fertilization by birds) was described for the Antarctic collections by Broady (1989). Prasiola calophylla was so far only confirmed from northeastern Atlantic and central European regions (Heesch et al. 2016). The position of P. calophylla as sister taxon to P. fluviatilis was also confirmed by Heesch et al. (2016). Thus, the earlier reports of P. calophylla from Antarctica have to be questioned.
The chloroplast morphology was determined by CLSM, as due to the dense cytoplasmic arrangement, it was difficult to observe the structure of the chloroplasts by light microscopy alone; particularly at the CLSM images, the division planes at the sides of the thalli were clearly visible, while anticlinal cell divisions took place at the cell periphery, leading to an elongation of the blades; periclinal cell divisions were observed more toward the centre of the blades. These periclinal cell divisions lead to broadening of the thalli; this division mode is basically the same for all Prasiola sp.; however, the regularity of the occurring of periclinal cell divisions leads to different degrees of curling (Richter et al. 2016).
To investigate the effect of experimentally applied water-limiting conditions, P. calophylla was either exposed to osmotically active sorbitol (Kaplan et al. 2012, 2013) or to 20% respectively 60% RH, resulting in desiccation. The small thalli collected in the field were densely packed with cytoplasm, exhibited a very low degree of vacuolization and required extremely high concentrations of sorbitol to induce incipient plasmolysis. In fact, plasmolysis in P. calophylla occurred only when exposed to 3000–4000 mM sorbitol. These concentrations translate into very low water potentials of −13 to −23.3 MPa (Kosugi et al. 2014). Y(II) was decreased to ∼60% of the initial value at 3000 mM, and at 4000 mM, Y(II) was completely suppressed. Similarly, in an Antarctic P. crispa, the effective quantum yield of PSII reached zero at 3000 mM sorbitol (Kosugi et al. 2010). In Trentepohlia aurea (Ulvophyceae) and some chlorolichens (e.g. Stereocaulon sorediiferum), however, electron flow through PSII remains active even at 4000 mM sorbitol (Kosugi et al. 2010, 2014).
In contrast, the aeroterrestrial streptophyte green alga Klebsormidium sp. was plasmolysed at 600–800 mM sorbitol (Kaplan et al. 2012). When compared with aquatic algae, even this value can be regarded as high. Moreover, in the late diverged streptophyte green alga Zygnema sp., which prefers hydroterrestrial habitats, incipient plasmolysis was found between 400 and 600 mM sorbitol. These data suggest that P. calophylla exhibits a constitutively very negative intracellular osmotic potential, reducing cellular water loss due to desiccation. The major inorganic and organic solutes that might contribute to the osmotic potential have been described as potassium, phosphate, amino acids and low molecular weight carbohydrates in P. crispa ssp. antarctica (Jacob et al. 1991). Another interesting phenomenon has been observed under high salinity treatments of P. crispa ssp. antarctica, which lead to the occurrence of vacuoles that regulate the volume change of the cells (Jacob et al. 1991); this phenomenon appears contradictory at the first glance, as one would expect water loss from the vacuoles. Yet, the mechanical benefit of this capacity was described in Porpyhra umbilicalis, which uses these vacuoles to mechanically avoid separation of the protoplasm from the cell wall (Wiencke and Läuchli 1981; Wiencke et al. 1984).
To complement osmotic experiments, we analysed the physiological parameters by continuous measurements of the effective quantum yield in field samples which were either collected on rainy or sunny days and exposed to ∼20% or ∼60% RH, respectively. The desiccation kinetics revealed two factors influencing the Y(II) of P. calophylla under water scarcity: (1) moist conditions (i.e. rainfall, high RH) prior to desiccation stress increased the maintenance of photosynthetic activity during desiccation, whereas a previous dry period caused an earlier inhibition. (2) Increasing the RH to ∼60% maintained the Y(II) for a longer interval despite a previous dry weather period. This suggests that short-term acclimation to dry conditions (i.e. hardening) plays only a minor role in P. calophylla to cope with low water availability, since dry weather periods do not increase the desiccation tolerance. Therefore, some constitutive tolerance mechanisms, such as an extremely negative osmotic potential inside the cells, might be particularly important to survive severe desiccation stress and to recover quickly after rehydration. This includes loss of PSII reaction centre activity combined with a strong reduction of both the photo-oxidation state and rereduction of PSI under dry conditions as suggested for P. crispa from an Antarctic habitat (Kosugi et al. 2010). Unlike light-induced NPQ, this desiccation-induced quenching is not restricted to light, as it also occurs in the dark (Nabe et al. 2007). It coincides with a strong reduction of fluorescence emitted between ∼680 and ∼780 nm (Kosugi et al. 2010), which was also indicated in the present study by visualizing the reduction of the chlorophyll autofluorescence, illustrated by confocal laser scanning microscopy where we choose 20% RH, to gain maximal desiccation. However, the chlorophyll autofluorescence increased again upon rehydration, indicating the recovery of this treatment. Analogous effects, i.e. the inactivation of PSII while PSI still maintains activity, were observed in Ulva sp., where PSI remained even active after exposure to 4000 mM sorbitol (Gao et al. 2011).
However, at the present state, we can only speculate about the extremely well performance under water-limiting conditions in P. calophylla; possible candidates for protection are, e.g. flavodiiron proteins, which are involved in cyanobacterial energy dissipation in photosynthesis; Flv1 and Flv3 proteins, functioning in the ‘Mehler-like’ reaction that protects PSI, have been found not only in cyanobacteria but also in green algae, mosses and lycophytes (e.g. Allahverdiyeva et al. 2015). Moreover, genes coding for flavodoxin, which is induced in cyanobacteria and green algae under osmotic-, heat- and high light stress, have been found in many Trebouxiophyceae (Karlusich et al. 2014). However, flavodoxins were lost during evolution and are lacking in streptophytic green algae and plants and tend to be lost in iron-rich habitats. In contrast, ferredoxins have been maintained throughout plant evolution. They are electron shuttlers harbouring iron-sulfur clusters and are particularly sensitive to desiccation, leading to downregulation of photosynthesis in streptopyhte green algae (e.g. Karlusich et al. 2015). However, up to now, only the draft plastid and mitochondrial genome sequences from P. crispa have been published (Carvalho et al. 2015); therefore, no direct evidence on the occurrence of these protection mechanisms in Prasiola is available.
The ultrastructural changes upon severe desiccation for 2.5 h corroborate our observations obtained by desiccation experiments. Untreated control cells already had a very dense cytoplasm, typical for terrestrial Prasiola sp. (e.g. Holzinger et al. 2006; Richter et al. 2016). This was even the case in the fully hydrated natural condition, as the samples for TEM were harvested on a rainy day. Interestingly, even under severe desiccation conditions (20% RH for 2.5 h; we choose this treatment to illustrate the effect of the maximal water loss), some thylakoid membranes remained intact, suggesting molecular protection mechanisms as realized in other green algae (Gasulla et al. 2013). However, the preservation of the ultrastructure of desiccated samples by chemical fixation has its limitations (see Holzinger et al. 2011); for future studies, high pressure freeze fixation followed by freeze substation should be applied, but even with this technique, it is not possible to freeze desiccated samples, and an intermedium (e.g. sucrose solution) has to be used. Given the fact of the extremely negative osmotic potential, rehydration prior to the freezing process could hardly be avoided. A similar condensed cytoplasm was observed in other green algae (Holzinger et al. 2011) or mosses (Pressel and Duckett 2010). A particularly interesting observation is the occurrence of vacuoles in 2.5 h desiccated cells. As discussed previously for P. crispa under osmotic stress (Jacob et al. 1992), the same phenomenon also occurs under desiccation; in P. crispa, it was assumed that the vacuoles are deposition sites for ions which enter the cells. Severe desiccation goes along with changes in the ionic homeostasis that needs to be buffered to some extent, and these emerging vacuoles might be a way to compensate for these increases.
Contribution of the cell walls
Furthermore, the cell walls of P. calophylla withstand the mechanical stress induced by desiccation, and the cytoplasm appears only slightly retracted in some parts. However, this was also the case in the non-desiccated group. In P. calophylla, we did not observe the desiccation-induced cell wall undulations observed in Klebsormidium sp. (Holzinger et al. 2011; Herburger and Holzinger 2015) or any inner pectic layers as in Ulva compressa (Holzinger et al. 2015). The thalli of U. compressa and P. calophylla show some similarities, such as a flat leaf-like appearance and highly ordered arrangement of cells. However, the morphological responses to desiccation stress followed by rehydration are different in the different genera, likely due to different cell wall architectures and chemistries. Individual Ulva cells are surrounded by a flexible pectic layer, followed by several cellulose- and xyloglucan-rich layers, which are embedded in an amorphous matrix consisting of pectins and ulvan (Lahaye and Robic 2007). This allows both, the protoplasts and the whole thallus to shrink and expand reversibly upon desiccation and rehydration, respectively, which minimizes structural und ultrastructural damage (Holzinger et al. 2015). In contrast, Trebouxiophyceae lack common pectic polymers found in Ulva (e.g. homogalacturonan; Domozych et al. 2012) and desiccation for up to 2.5 h at 20% RH did not deform thalli of P. calophylla significantly. As shown for Prasiola japonica, Prasiola cell walls consist of at least four chemically distinguishable layers (Takeda et al. 1967). Interestingly, the innermost layer surrounding the protoplast contains mainly cellulose, followed by a thick and stable mannan-rich layer, while acidic polysaccharides (e.g. uronic acid) are restricted to thin layers in anticlinal walls between individual cells (Takeda et al. 1967). Embedding the protoplasts and individual cells into a rigid matrix appears to reduce mechanical deformation upon water loss and restricts structural damage to a minimum as shown for rehydrated thalli. In combination with a high water holding capacity of the protoplasts due to low osmotic pressures, structural damage as a consequence of water scarcity can be minimized. Thalli of U. compressa did not recover their photosynthetic performance after 90 min of desiccation at ∼62% RH, corresponding to 27% relative water content (RWC) of the thalli. Although the RWC was not measured in P. calophylla, we can estimate that in the present study, we have more severely desiccated the P. calophylla thalli, since they were exposed to ∼20% RH for over 2.5 h. Despite maintaining the desiccated state for at least 40 min, all samples completely recovered.
Prasiola crispa, occurring in the littoral zone of the Antarctic sea, was able to survive a water loss of 86% after desiccation at 50% RH for 14 days (Jacob et al. 1992). In addition to very low osmotic pressure inside the protoplast, the high water holding capacity of Prasiola thalli might also be attributed to a cuticle-like layer on the thallus surface, as shown by nile red staining, a fluorescence dye commonly used to visualize neutral lipids being part of cuticles (Broehan et al. 2013). As shown for the brown alga Fucus distichus, this cuticle-like layer might consist of unsaturated fatty acids (Quadir et al. 1979). Interestingly, in P. crispa, a relation between excessive lipid bodies and fatty acids incorporated into the cell wall was assumed (Jacob 1992); since these cytoplasmic lipid bodies remain stable even after cultivation for several months in the dark, a functional role as storage compounds is unlikely. Instead, they might contain the building blocks for the cuticle-like layer. This hypothesis is supported by the observation that Prasiola species occurring in terrestrial or littoral habitats, where desiccation stress occurs frequently, usually contain abundant lipid bodies (this study; Jacob et al. 1992; Holzinger et al. 2006). Accumulating lipid bodies functioning as storage compounds in green algae is usually related to nitrogen deprivation (Pichrtová et al. 2014), which can be excluded for the organism investigated in the present study, since a high nitrogen input due to dog excrements is considered to contribute to the abundant growth (Hartmann et al. 2016). Nitrogen is also essential for biosynthesis of the N-containing UV sunscreen prasiolin (Hartmann et al. 2016).
Besides water availability, the temperature regime in the habitat strongly influences the photosynthetic performance of terrestrial algae, with low temperatures reducing the catalytic activity of enzymes involved in the Calvin-Benson cycle. As a consequence, electron carriers of the photosynthetic apparatus might be overreduced, which leads to a reduction of the photosynthetic performance. In P. calophylla, the highest rETR in response to increasing photon fluence rates occurred at 25 °C, while the activity was lower at 10 and 5 °C. We have intentionally exposed samples collected on a sunny day to the various temperatures, as elevated temperatures are expected under these conditions. Obviously, 45 °C represents a threshold for physiological activities. A similar temperature dependence was found in an Antarctic P. crispa, where the Y(II) declined below 15 and above 25 °C (Kosugi et al. 2010), pointing to similar physiological traits.
The present study gives a detailed overview on the physiological capabilities and ultrastructure of P. calophylla, a terrestrial alga that tolerates a high degree of water loss in its vegetative state. Field-collected samples showed very little sensitivity to desiccation or osmotic water reduction, enabling them to survive under extreme water-limiting conditions. The unaffected ultrastructure of the cells contributes to this high tolerance. The underlying molecular mechanisms, however, are poorly studied and, hence, P. calophylla would represent a perfect model system for metabolomic or transcriptomic changes under desiccation stress.
Open access funding provided by Austrian Science Foundation (FWF). This study was supported by FWF grants P 24242-B16 and I 1951-B16 to A.H., DFG grant KA899/16-1/4 to U.K. and USA NSF award DEB-1036448 to L.L. We thank Sabrina Obwegeser, MSc., and Beatrix Jungwirth, University of Innsbruck, for excellent technical help in TEM sectioning and image generation.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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