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Protoplasma

, Volume 254, Issue 3, pp 1421–1429 | Cite as

Bacteriomes of the corn leafhopper, Dalbulus maidis (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor Sulcia symbiont: molecular characterization, ultrastructure, and transovarial transmission

  • María Eugenia BrentassiEmail author
  • Ernesto Franco
  • Pedro Balatti
  • Rocío Medina
  • Franco Bernabei
  • Ana M. Marino de Remes Lenicov
Original Article

Abstract

In this study, we surveyed the bacteriome-associated microbiota of the corn leafhopper Dalbulus maidis by means of histological, ultrastructural, and molecular analyses. Amplification and sequencing of 16S rDNA genes revealed that the endosymbiont “Candidatus Sulcia muelleri” (Phylum Bacteroidetes) resides in bacteriomes of D. maidis. Phylogenetic analysis showed that the sequence was closely allied to others found in representatives of the subfamily Deltocephalinae. We failed to amplify other sequences as “Candidatus Nasuia deltocephalinicola,” a co-primary symbiont frequently associated to deltocephaline leafhoppers. In addition, a metagenetic analysis carried out in order to investigate the presence of other bacteriome-associated bacteria of D. maidis showed that the sequence of Sulcia accounted for 98.56 % of all the sequences. Histological and ultrastructural observations showed that microorganisms harbored in bacteriomes (central syncytium and cytoplasm of uninucleate bacteriocytes) look like others Sulcia described in hemipteran species and they were transovarially transmitted from mother to offspring which is typical of obligate endosymbionts. The only presence of Sulcia in the bacteriomes of D. maidis was discussed.

Keywords

Candidatus Sulcia muelleri” Bacteriomes Corn stunt Corn leafhopper Obligate endosymbionts 

Notes

Acknowledgments

We thank Dr. E. Virla for collecting D. maidis samples used in the present work and Dr. A Maciá for improving the English version of this manuscript. The authors especially thank Dr Hiroaki Noda for providing Nephotettix cincticeps specimens. This work was supported by FONCYT-PICT-2007-00143-03, Comisión de Investigaciones Científicas Pcia de Bs. As. (CIC), Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), and Universidad Nacional de La Plata (UNLP).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  1. 1.División Entomología. Facultad de Ciencias Naturales y MuseoUniversidad Nacional de La Plata (UNLP)La PlataArgentina
  2. 2.Comisión de Investigaciones Científicas, Pcia de Bs. As. (CIC) and Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET)La PlataArgentina
  3. 3.Centro de Investigaciones de Fitopatología (CIDEFI). Facultad de Ciencias Agrarias y ForestalesUNLP-CICLa PlataArgentina
  4. 4.Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI). Facultad de Ciencias ExactasUNLP-CONICETLa PlataArgentina

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