Protoplasma

, Volume 253, Issue 6, pp 1439–1447

Biocompatibility of different nanostructured TiO2 scaffolds and their potential for urologic applications

  • Roghayeh Imani
  • Meysam Pazoki
  • Daša Zupančič
  • Mateja Erdani Kreft
  • Veronika Kralj-Iglič
  • Peter Veranič
  • Aleš Iglič
Original Article

DOI: 10.1007/s00709-015-0896-0

Cite this article as:
Imani, R., Pazoki, M., Zupančič, D. et al. Protoplasma (2016) 253: 1439. doi:10.1007/s00709-015-0896-0

Abstract

Despite great efforts in tissue engineering of the ureter, urinary bladder, and urethra, further research is needed in order to improve the patient’s quality of life and minimize the economic burden of different lower urinary tract disorders. The nanostructured titanium dioxide (TiO2) scaffolds have a wide range of clinical applications and are already widely used in orthopedic or dental medicine. The current study was conducted to synthesize TiO2 nanotubes by the anodization method and TiO2 nanowires and nanospheres by the chemical vapor deposition method. These scaffolds were characterized with scanning electron microscopy (SEM) and X-ray diffraction (XRD) methods. In order to test the urologic applicability of generated TiO2 scaffolds, we seeded the normal porcine urothelial (NPU) cells on TiO2 nanotubes, TiO2 nanowires, TiO2 nanospheres, and on the standard porous membrane. The viability and growth of the cells were monitored everyday, and after 3 weeks of culturing, the analysis with scanning electron microscope (SEM) was performed. Our results showed that the NPU cells were attached on all scaffolds; they were viable and formed a multilayered epithelium, i.e., urothelium. The apical plasma membrane of the majority of superficial NPU cells, grown on all three different TiO2 scaffolds and on the porous membrane, exhibited microvilli; thus, indicating that they were at a similar differentiation stage. The maximal caliper diameter measurements of superficial NPU cells revealed significant alterations, with the largest cells being observed on nanowires and the smallest ones on the porous membrane. Our findings indicate that different nanostructured TiO2 scaffolds, especially nanowires, have a great potential for tissue engineering and should be further investigated for various urologic applications.

Keywords

Nanostructured TiO2 scaffolds Anodization Chemical vapor deposition Normal porcine urothelial cells Urologic application 

Funding information

Funder NameGrant NumberFunding Note
Slovenian Research Agency ARRS
  • P2-0232
  • P3-0108

Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  • Roghayeh Imani
    • 1
    • 2
  • Meysam Pazoki
    • 3
  • Daša Zupančič
    • 4
  • Mateja Erdani Kreft
    • 4
  • Veronika Kralj-Iglič
    • 2
  • Peter Veranič
    • 4
  • Aleš Iglič
    • 1
  1. 1.Laboratory of Biophysics, Faculty of Electrical EngineeringUniversity of LjubljanaLjubljanaSlovenia
  2. 2.Laboratory of Clinical Biophysics, Faculty of Health SciencesUniversity of LjubljanaLjubljanaSlovenia
  3. 3.Department of Chemistry, Ångström Laboratory, Physical ChemistryUppsala UniversityUppsalaSweden
  4. 4.Institute of Cell Biology, Faculty of MedicineUniversity of LjubljanaLjubljanaSlovenia

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