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Protoplasma

, Volume 253, Issue 5, pp 1373–1383 | Cite as

Effects of fusaric acid treatment on the protocorm-like bodies of Dendrobium sonia-28

  • Raheleh Dehgahi
  • Latiffah Zakaria
  • Azhar Mohamad
  • Alireza Joniyas
  • Sreeramanan Subramaniam
Original Article

Abstract

Dendrobium sonia-28 is a popular orchid hybrid due to its flowering recurrence and dense inflorescences. Unfortunately, it is being decimated by fungal diseases, especially those caused by Fusarium proliferatum. In this study, selection of F. proliferatum-tolerant protocorm-like bodies (PLBs) was carried out by assessing the effects of differing concentrations of fusaric acid (FA). PLBs were cultured on Murashige and Skoog (MS) medium supplemented with 0.05 to 0.2 millimolar (mM) concentrations of FA. Higher concentrations of FA increased mortality of PLBs and reduced their growth. The survival rate for 0.05 mM FA was 20 % but only 1 % at the highest dose of 0.2 mM. Additionally, two different size ranges of PLBs were investigated, and growth increased more at lower FA concentrations for larger PLBs, whilst the growth rate of smaller PLBs was inhibited at an FA concentration of 0.2 mM. Histological examination using transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analyses disclosed severe cell wall and organelle damage, as well as stomatal closure in PLBs treated with the high FA concentrations. Reductions in plantlet growth were much greater at the highest concentrations of FA. Some randomly amplified polymorphic DNA (RAPD) markers clearly discriminated between selected and non-selected variants of Dendrobium sonia-28, showing different banding patterns for each FA concentration and specific bands for selected and control plants.

Keywords

Dendrobium sonia-28 Somaclonal variation PLBs Fusaric acid 

Abbreviations

MS

Murashige and Skoog

PLBs

Protocorm-like bodies

FA

Fusaric acid

mM

Millimolar

RAPD

Randomly amplified polymorphic DNA

Notes

Acknowledgments

The authors are grateful to USM Research University 2012 (1001/PBIOLOGI/811211) for funding this project.

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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  • Raheleh Dehgahi
    • 1
  • Latiffah Zakaria
    • 1
  • Azhar Mohamad
    • 2
  • Alireza Joniyas
    • 1
  • Sreeramanan Subramaniam
    • 1
  1. 1.School of Biological SciencesUniversiti Sains MalaysiaGeorgetownMalaysia
  2. 2.Agrotechnology and Bioscience DivisionMalaysian Nuclear AgencyBangiMalaysia

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