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Protoplasma

, Volume 252, Issue 2, pp 591–603 | Cite as

Origination of asexual plantlets in three species of Crassulaceae

  • Jiansheng Guo
  • Hailiang Liu
  • Yangyang He
  • Xianghuan Cui
  • Xiling Du
  • Jian ZhuEmail author
Original Article

Abstract

During asexual plant reproduction, cells from different organs can be reprogrammed to produce new individuals, a process that requires the coordination of cell cycle reactivation with the acquisition of other cellular morphological characteristics. However, the factors that influence the variety of asexual reproduction have not yet been determined. Here, we report on plantlet formation in Kalanchoe daigremontiana, Graptopetalum paraguayense, and Crassula portulacea (Crassulaceae) and analyse the effect of initiating cells on asexual reproduction in these three species. Additionally, the roles of WUSCHEL (WUS) and CUP-SHAPED COTYLEDON 1 (CUC1) in the asexual reproduction of these species were analysed through qRT-PCR. Our results indicated that pre-existing stem cell-like cells at the sites of asexual reproduction were responsible for the formation of plantlets. These cells were arrested in different phases of the cell cycle and showed different cell morphological characteristics and cell counts. The accumulation of auxin and cytokinin at the sites of asexual plantlet formation indicated their important functions, particularly for cell cycle reactivation. These differences may influence the pattern and complexity of asexual reproduction in these Crassulaceae species. Additionally, the dynamic expression levels of CUC1 and WUS may indicate that CUC1 functions in the formation of callus and shoot meristems; whereas, WUS was only associated with shoot induction.

Keywords

Kalanchoe daigremontiana Graptopetalum paraguayense Crassula portulacea Asexual reproduction Ultrastructure Cell cycle 

Notes

Acknowledgments

This research was supported by the National Natural Sciences Foundation of China (nos. 31370214, 31301058), the Major State Basic Research Development Program (973 Program) of China (No. 2012CB966903), and the Key Programme for Basic Research of the Science and Technology Commission of Shanghai (13JC1407102).

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Jiansheng Guo
    • 1
  • Hailiang Liu
    • 2
    • 3
  • Yangyang He
    • 1
  • Xianghuan Cui
    • 1
  • Xiling Du
    • 1
  • Jian Zhu
    • 1
    Email author
  1. 1.Department of Molecular and Cell Biology, School of Life Science and TechnologyTongji UniversityShanghaiChina
  2. 2.Translational Center for Stem Cell ResearchTongji University School of Medicine, Tongji HospitalShanghaiChina
  3. 3.College of Life Science of Shihezi UniversityShiheziChina

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