Protoplasma

, Volume 251, Issue 4, pp 931–941 | Cite as

Assessment of cell cycle phase-specific effects of zerumbone on mitotically synchronous surface cultures of Physarum polycephalum

  • Iyyappan Rajan
  • Remitha Rabindran
  • N. Nithya
  • T. Lakshmipriya
  • P. R. Jayasree
  • P. R. M. Kumar
Original Article
First Online:
Received:
Accepted:

Abstract

Zerumbone, a natural cyclic sesquiterpene, has been the focus of recent research as it has been found to exhibit selective toxicity towards cancer cells compared to normal cells. Studies on the cell cycle phase-specific effects of this interesting compound, however, remain sparse. Hence, concentration and time-dependent effects of zerumbone were evaluated employing a suitable model system, the naturally synchronous surface cultures of Physarum polycephalum. Zerumbone treatment in S, early, and late G2 phases resulted in G2 arrest. Early G2 phase exhibited the highest sensitivity (P < 0.001) to the compound. Protein profiles showed a complete inhibition of cyclin B1 expression following zerumbone treatment. Furthermore, FACS and comet analysis revealed that zerumbone inhibited DNA synthesis (P < 0.001) without being genotoxic at the concentrations tested. Differential display of mRNA showed distinct zerumbone-induced variations in transcript profiles, an analysis of which suggested a likely link between cellular networks involving stress-related gene expression and G2 arrest in P. polycephalum.

Keywords

Physarum polycephalum Zerumbone G2 arrest G2 release Cyclin B1 DDRT-PCR 
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Notes

Acknowledgments

Thanks are due to the Director and Dr. V.T. Santhosh Kumar, RGCB, Thiruvananthapuram, for use of FACS facility and the Department of Biotechnology (Government of India) for the financial assistance to JPR through BioCare Scheme.

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Iyyappan Rajan
    • 1
  • Remitha Rabindran
    • 1
  • N. Nithya
    • 1
  • T. Lakshmipriya
    • 1
  • P. R. Jayasree
    • 1
  • P. R. M. Kumar
    • 1
  1. 1.Department of BiotechnologyUniversity of CalicutKeralaIndia

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