Protoplasma

, Volume 251, Issue 3, pp 639–648 | Cite as

Chloroplast molecular farming: efficient production of a thermostable xylanase by Nicotiana tabacum plants and long-term conservation of the recombinant enzyme

  • Laura Pantaleoni
  • Paolo Longoni
  • Lorenzo Ferroni
  • Costanza Baldisserotto
  • Sadhu Leelavathi
  • Vanga Siva Reddy
  • Simonetta Pancaldi
  • Rino Cella
Original Article

Abstract

The high cost of recombinant enzymes for the production of biofuel from ligno-cellulosic biomass is a crucial factor affecting the economic sustainability of the process. The use of plants as biofactories for the production of the suitable recombinant enzymes might be an alternative to microbial fermentation. In the case of enzyme accumulation in chloroplasts, it is fundamental to focus on the issue of full photosynthetic efficiency of transplastomic plants in the field where they might be exposed to abiotic stress such as high light intensity and high temperature. Xylanases (EC 3.2.1.8), a group of enzymes that hydrolyse linear polysaccharides of beta-1,4-xylan into xylose, find an application in the biofuel industry favouring biomass saccharification along with other cell-wall degrading enzymes. In the present study, we analysed how a high level of accumulation of a thermostable xylanase in tobacco chloroplasts does not impact on photosynthetic performance of transplastomic plants grown outdoors. The recombinant enzyme was found to be stable during plant development, ex planta and after long-term storage.

Keywords

Chloroplast transformation Enzyme long-term storage Photosynthetic performance of tobacco transplastomic plants Plant biofactory Recombinant thermostable xylanase 

Abbreviations

BSX

Bacillus sp. xylanase

TSP

Total soluble proteins

Chl

Chlorophyll

Car

Carotenoids

FM

Maximum PSII fluorescence in the dark-adapted state

F0

Minimum fluorescence in the dark-adapted state

Fv

Variable fluorescence

PAM

Pulse amplitude modulated fluorimetry

PSII

Photosystem II

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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Laura Pantaleoni
    • 1
  • Paolo Longoni
    • 1
    • 4
  • Lorenzo Ferroni
    • 2
  • Costanza Baldisserotto
    • 2
  • Sadhu Leelavathi
    • 3
  • Vanga Siva Reddy
    • 3
  • Simonetta Pancaldi
    • 2
  • Rino Cella
    • 1
  1. 1.Department of Biology and BiotechnologyUniversity of PaviaPaviaItaly
  2. 2.Department of Life Sciences and BiotechnologiesUniversity of FerraraFerraraItaly
  3. 3.Plant Transformation GroupInternational Centre for Genetic Engineering and BiotechnologyNew DelhiIndia
  4. 4.Department of Botany and Plant BiologyUniversity of Geneva, Sciences IIIGeneve 4Switzerland

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