, Volume 250, Issue 2, pp 483–493 | Cite as

Hepatoprotective effect of Origanum vulgare in Wistar rats against carbon tetrachloride-induced hepatotoxicity

  • Mohammad Sikander
  • Shabnam Malik
  • Kehkashan Parveen
  • Maqsood Ahmad
  • Deepak Yadav
  • Zubair Bin Hafeez
  • Manish Bansal
Original Article


The effect of an aqueous extract of Origanum vulgare (OV) leaves extract on CCl4-induced hepatotoxicity was investigated in normal and hepatotoxic rats. To evaluate the hepatoprotective activity of OV, rats were divided into six groups: control group, O. vulgare group, carbon tetrachloride (CCl4; 2 ml/kg body weight) group, and three treatment groups that received CCl4 and OV at doses of 50, 100, 150 mg/kg body weight orally for 15 days. Alanine amino transferase (ALT), alkaline phosphatase (ALP), and aspartate amino transferase (AST) in serum, lipid peroxide (LPO), GST, CAT, SOD, GPx, GR, and GSH in liver tissue were estimated to assess liver function. CCl4 administration led to pathological and biochemical evidence of liver injury as compared to controls. OV administration led to significant protection against CCl4-induced hepatotoxicity in dose-dependent manner, maximum activity was found in CCl4 + OV3 (150 mg/kg body weight) groups and changes in the hepatocytes were confirmed through histopathological analysis of liver tissues. It was also associated with significantly lower serum ALT, ALP, and AST levels, higher GST, CAT, SOD, GPx, GR, and GSH level in liver tissue. The level of LPO also decreases significantly after the administration of OV leaves extract. The biochemical observations were supplemented with histopathological examination of rat liver sections. Thus, the study suggests O. vulgare showed protective activity against CCl4-induced hepatotoxicity in Wistar rats and might be beneficial for the liver toxicity.


Hepato toxicity Carbon tetrachloride Origanum vulgare Oxidative stress Lipid peroxidation 



Dr. Mohammad Sikander is thankful to Dr. Ashok Mukherji (All India Institute of Medical Science) for the necessary helps to conduct this study.

Conflict of interest statement

The authors declare that there are no conflicts of interest.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Mohammad Sikander
    • 1
  • Shabnam Malik
    • 1
  • Kehkashan Parveen
    • 2
  • Maqsood Ahmad
    • 3
  • Deepak Yadav
    • 4
  • Zubair Bin Hafeez
    • 1
  • Manish Bansal
    • 5
  1. 1.Department of BiotechnologyHamdard UniversityNew DelhiIndia
  2. 2.Department of BiochemistryJamia HamdardNew DelhiIndia
  3. 3.Department of Pharmacognosy & Phytochemistry, Faculty of PharmacyHamdard UniversityNew DelhiIndia
  4. 4.Department of Ilmul Adivia Faculty of MedicineHamdard UniversityNew DelhiIndia
  5. 5.National Research Center On EquinesHisarIndia

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