The role of calcium in chloroplasts—an intriguing and unresolved puzzle
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More than 70 years of studies have indicated that chloroplasts contain a significant amount of calcium, are a potential storage compartment for this ion, and might themselves be prone to calcium regulation. Many of these studies have been performed on the photosynthetic light reaction as well as CO2 fixation via the Calvin–Benson–Bassham cycle, and they showed that calcium is required in several steps of these processes. Further studies have indicated that calcium is involved in other chloroplast functions that are not directly related to photosynthesis and that there is a calcium-dependent regulation similar to cytoplasmic calcium signal transduction. Nevertheless, the precise role that calcium has as a functional and regulatory component of chloroplast processes remains enigmatic. Calcium concentrations in different chloroplast subcompartments have been measured, but the extent and direction of intra-plastidal calcium fluxes or calcium transport into and from the cytosol are not yet very well understood. In this review we want to give an overview over the current knowledge on the relationship between chloroplasts and calcium and discuss questions that need to be addressed in future research.
KeywordsCalcium Signaling Chloroplasts Photosynthesis Calvin–Benson cycle Calmodulin
Genetically encoded calcium indicator
Guanosine 5′-triphosphate (or 5′-diphosphate) 3′-diphosphate
Component of PSI/II
Translocon on the inner envelope of chloroplast
This work has been funded by the EU within the Marie-Curie ITN COSI (ITN 2008 GA 215-174) and by the Deutsche Forschungsgemeinschaft via ERA-NETs “Plant Genomics” (ERAPG 08-044).
Conflict of interest
The authors declare that they have no conflict of interest.
- Balsera M, Goetze TA, Kovacs-Bogdan E, Schurmann P, Wagner R, Buchanan BB, Soll J, Bolter B (2009) Characterization of Tic110, a channel-forming protein at the inner envelope membrane of chloroplasts, unveils a response to Ca(2+) and a stromal regulatory disulfide bridge. J Biol Chem 284(5):2603–2616. doi: 10.1074/jbc.M807134200 PubMedCrossRefGoogle Scholar
- Bayer RG, Stael S, Rocha A, Mair A, Vothknecht UC, Teige M (2011b) Chloroplast localised protein kinases—a step forward towards a complete inventory. J Exp Bot. doi: 10.1093/jxb/err377
- Chigri F, Flosdorff S, Pilz S, Kolle E, Dolze E, Gietl C, Vothknecht UC (2011) The Arabidopsis calmodulin-like proteins AtCML30 and AtCML3 are targeted to mitochondria and peroxisomes, respectively. Plant Mol Biol. doi: 10.1007/s11103-011-9856-z
- Han S, Tang R, Anderson LK, Woerner TE, Pei Z-M (2003) A cell surface receptor mediates extracellular Ca2+ sensing in guard cells. Nature 425(6954):196–200, http://www.nature.com/nature/journal/v425/n6954/suppinfo/nature01932_S1.html PubMedCrossRefGoogle Scholar
- Jordan P, Fromme P, Witt HT, Klukas O, Saenger W, Krausz N (2001) Three-dimensional structure of cyanobacterial photosystem I at 2.5[thinsp][angst] resolution. Nature 411(6840):909–917, http://www.nature.com/nature/journal/v411/n6840/suppinfo/411909a0_S1.html PubMedCrossRefGoogle Scholar
- Masuda S, Tozawa Y, Ohta H (2008) Possible targets of “magic spots” in plant signalling. Plant Signal Behav 3(11):1021–1023Google Scholar
- Mehlmer N, Parvin N, Hurst CH, Knight MR, Teige M, Vothknecht UC (2011) A toolset of aequorin expression vectors for in planta studies of subcellular calcium concentrations in Arabidopsis thaliana. J Exp Bot. doi: 10.1093/jxb/err406
- Nobel PS, Murakami S, Takamiya A (1966) Localization of light-induced strontium accumulation in spinach chloroplasts. Plant Cell Physiol 7(2):263–275Google Scholar
- Stael S, Rocha AG, Robinson AJ, Kmiecik P, Vothknecht UC, Teige M (2011b) Arabidopsis calcium-binding mitochondrial carrier proteins as potential facilitators of mitochondrial ATP-import and plastid SAM-import. FEBS Lett 585(24):3935–3940. doi: 10.1016/j.febslet.2011.10.039 PubMedCrossRefGoogle Scholar
- Stael S, Rocha AG, Wimberger T, Anrather D, Vothknecht UC, Teige M (2011c) Crosstalk between calcium signalling and protein phosphorylation at the thylakoid membrane. J Exp Bot. doi: 10.1093/jxb/err394
- Vainonen JP, Sakuragi Y, Stael S, Tikkanen M, Allahverdiyeva Y, Paakkarinen V, Aro E, Suorsa M, Scheller HV, Vener AV, Aro EM (2008) Light regulation of CaS, a novel phosphoprotein in the thylakoid membrane of Arabidopsis thaliana. FEBS J 275(8):1767–1777. doi: 10.1111/j.1742-4658.2008.06335.x PubMedCrossRefGoogle Scholar
- Wang W-H, Yi X-Q, Han A-D, Liu T-W, Chen J, Wu F-H, Dong X-J, He J-X, Pei Z-M, Zheng H-L (2011) Calcium-sensing receptor regulates stomatal closure through hydrogen peroxide and nitric oxide in response to extracellular calcium in Arabidopsis. J Exp Bot. doi: 10.1093/jxb/err259