Distribution and function of epistomatal mucilage plugs
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Investigation of 67 gymnosperm and angiosperm species belonging to 25 orders shows that epistomatal mucilage plugs are a widespread phenomenon. Measurements of the leaf water status by using the leaf patch clamp pressure technique suggest that the mucilage plugs are involved in moisture uptake and buffering leaf cells against complete turgor pressure loss at low humidity.
KeywordsMucilage Epistomatal plugs Leaf water status Turgor pressure Water ascent Patch clamp pressure
Acid mucopolysaccharides have been detected in the xylem vessels of higher plant species, particularly in trees (Zimmermann et al. 2004). Drought and salinity stress enforce xylem-bound mucilage formation (Zimmermann et al. 1994, 2002; Zimmermann et al. 2007a). Xylem-bound mucilage is most probably involved in water lifting against gravity (Plumb and Bridgman 1972; Pollack 2001; Zimmermann et al. 2004; Yeo and Flowers 2007). Recent studies have shown (Zimmermann et al. 2007a) that transpirational water loss and moisture uptake from the atmosphere are also modulated by mucilage. In some species (e.g. Eucryphia cordifolia and Astronium fraxinifolium) the leaf surface is covered by a layer of acid mucopolysaccharides. Epistomatal cavities filled with mucilage are chararacteristic for the leaves of the salt-tolerant trees A. fraxinifolium and Bulnesia sarmientoi as well as for the salt-intolerant trees Populus nigra, Eucalyptus pilularis and Nothofagus dombeyi. Zimmermann et al. (2007a) gave a large body of evidence that epistomatal mucilage plugs and acid mucilage layers are not only able to buffer changes in leaf water status when transpiration is high, but also facilitate moisture uptake from the atmosphere (including fog, rain, ascending transpirational water from lower leaves and evaporation from the soil). Via foliar moisture uptake apical leaves of tall trees are immediately supplied with water, whereas water uptake from the roots needs many days (up to 4 weeks; Woodward 2004).
In this short communication we will show that epistomatal mucilage plugs (and mucilage surface layers) are a widespread phenomenon found in higher plants and trees growing on fog-laden coasts, in subtropical and tropical rainforests, but also in temperate zones upon exposure to drought.
Materials and methods
Leaves of tropical, subtropical and deciduous shrubs and trees were collected at their common habitat in Central Europe, Chile/Argentina, Crete, South-East Asia, Australia and New Zealand, respectively. Immediately after collection leaves were incubated in an aqueous 0.5% alcian blue solution (pH 3) for 24 h. The leaves were then rinsed very carefully with distilled water or with 3% acetic acid solution to remove excess alcian blue. Cross-sections and surface sections of the stained leaf pieces were made by hand. Sections were inspected by using a transmitted-light microscope (BX51; Olympus, Hamburg, Germany) equipped with a digital camera or a 3-D digital incident-light microscope (VHX-100; Keyence, Osaka, Japan).
Leaf water status
Leaf water status (i.e. turgor pressure) was measured online using the non-invasive leaf patch clamp pressure probe. The principle of the probe and the theoretical background of the parameter measured by the probe are described in details elsewhere (Zimmermann et al. 2008; Westhoff et al. 2009). Briefly, the leaf is positioned in the space between the two planar circular pads of the probe. A miniaturised, temperature-independent pressure sensor chip is integrated in one of the pads. A constantly kept, external clamp pressure (up to 250 kPa) is applied by a spring (Zimmermann et al. 2008) or a magnet (Westhoff et al. 2008) to the clamped leaf patch. Physics shows that the output pressure PP measured by the sensor is dictated by the pressure transfer function of the clamped leaf patch. The transfer function is related to the compressibility of the leaf patch and therefore varies exclusively with cell turgescence for a leaf of a given species. At high turgor pressure the transfer function assumes values close to zero, i.e. Pp is small. At very low turgor pressure the transfer function assumes values close to unity, i.e. the applied pressure is transferred to the sensor at most and Pp assumes a maximum value. In other words, Pp measured by the pressure sensor is inversely correlated to turgor pressure. This was shown by calibration of the patch clamp pressure probe against the cell turgor pressure probe (Zimmermann et al. 2008).
Results and discussion
Gymnosperms showed a larger tendency to form plugs than angiosperms. The plug density both on the adaxial and abaxial side of the species of the order Pinales was 72% ± 26% (n = 9). Evergreen species showed generally a higher mucilage plug density than deciduous or semi-evergreen species (56% ± 29%; n = 37 versus 42% ± 22%; n = 17). Examples for high plug density are Bulnesia arborea, Tristania sumatrana (Fig. 1e), Eucalyptus pilularis and Podocarpus nubigenus. The plug density of leaves of trees of tropical broadleaf rainforest and monsoon forest as well as subtropical broadleaf and needleleaf forest was frequently very high (average plug density: 66% ± 29%; n = 37). More than half of these trees (c. 62%) showed an epistomatal mucilage plug density of 60 - 100% of the stomata (e.g. Astronium fraxinifolium, Tibouchina mutabilis and Veronica lasiocarpa). Plug densities of this magnitude were only found in about one third of evergreen broadleaf, deciduous trees and shrubs, and broadleaf deciduous woodland of the temperate zones and the Mediterranean region (e.g. Eucalyptus globulus, Ficus carica and Quercus coccifera). The alcian blue precipitates of the leaves of these trees were also not as concise as in the case of leaves of trees growing in the tropical and subtropical forests, but still clearly identifiable. Extended studies on Populus nigra in Germany indicated a seasonal dependency of the plug density which resulted most likely from drought. Between May and June, 2006, no plugs could be detected on 25 m tall trees growing at the river Tauber, Germany, and on a hill c. 1 km away (18 May, T = 11 - 15°C, R.H. = 85 - 95% and 6 June, T = 10 - 14°C, R.H. = 68 - 98%). Plugs were first found on 20 June 2006 (plug density c. 5 - 10%). The number increased during the following two very dry and hot months (18 July, tree at the river Tauber, 40 - 50% plug density, T = 25 - 32°C and R.H. = 35 - 55%; tree on the near-by hill, 60 - 70% plug density, T = 32 - 35°C, R.H. = 21 - 29%). At the end of August the tree at the river Tauber also exhibited a similar plug density as the tree on the hill. The stomata density of the trees at both locations was with 75 ± 24 stomata per mm2 (n = 60) comparable. A dependency of plug density on water supply was also found for Vitis vinifera (Fig. 1c) and Eucalyptus pilularis. Grapevines watered daily in Kiryat Gat, Israel exhibited no or few plugs whereas non-irrigated or only weekly irrigated grapevines (Würzburg, Germany and Gedera, Israel, respectively) exhibited high plug densities (June 2007, Kyriat Gat, less than 5% ± 1% (n = 173) plug density, T = 15 - 40°C and R.H. = 21 - 100%; June 2007, Gedera, 28% ± 15% (n = 87) plug density, T = 13 - 40°C and R.H. = 28 - 100%; August 2007, Würzburg, 65% ± 20% (n = 70) plug density, T = 6 - 35°C and R.H. = 8 - 100%). Similarly, a well-watered E. pilularis tree growing in a garden in New South Wales, Australia, showed only a few epistomatal mucilage plugs (plug density 50% on the abaxial side; the adaxial side did not contain stomata) compared to the high plug density found for trees of similar height growing at the edge of a relatively dry forest (depending on the age the plug density reached up to 100% on the abaxial and c. 15% on the adaxial side). Trees which could reach heights of 50 m and more (e.g. E. pilularis and Agathis australis (Figs. 1b and d)) showed an increase of plug density with height suggesting that their formation is enhanced once water supply from the roots is limited (E. pilularis, plug density 80 - 100% at 55 m height, 9 March 2006, T = 18 - 28°C, R.H. = 60 - 100%). This is also supported by the finding that the abaxial stomatal cavities of young leaves of 30 - 60 m tall E. pilularis were less filled with plugs than older ones (5% to 50 % versus 80% to 100%).
An interesting result is also the finding that Podocarpus totara (Fig. 1h) being endemic to New Zealand and Podocarpus nubigenus being endemic to South-Chile had a high capacity for plug formation. Both species are growing in a comparable ecosystem. Two further examples for high plug densities in species belonging to the same genus are Agathis australis (Figs. 1b and d), being endemic to the northern island of New Zealand, and Agathis robusta being endemic to South-East-Queensland, Australia, as well as Nothofagus dombeyi (Fig. 1f) being endemic to South-Chile/Argentina and Nothofagus truncata being endemic to New Zealand. By contrast, leaves of Griselinia littoralis being endemic to New Zealand exhibited high plug densities, whereas leaves of Griselinia scandens being endemic to South-Chile showed no plugs even though they grow in a comparable biome. These findings obviously demonstrate that the capability for epistomatal mucilage and mucilage layer formation not always continued after the fragmentation of the supercontinent Pangaea into the present continents during the Triassic period (200 million years ago). Reasons for this may be different microclimate conditions.
In the light of the above results we can conclude that epistomatal mucilage plugs (and layers) are a widespread phenomenon. Water uptake and binding of acid mucopolysaccharides are extremely high, in particular in the presence of di-(multi-)valent cations (Morse 1990; McCully and Boyer 1977; Esch et al. 1999; Zimmermann et al. 2007b). This suggests that epistomatal mucilage plugs (and layers) are involved in moisture uptake and particularly in buffering the leaves against rapid and excessive turgor pressure loss at low relative humidity. Support for this assumption is given by leaf patch clamp pressure measurements. Plots of the output pressure Pp values measured on non-irrigated (mucilage plug-rich) and irrigated (mucilage plug-free) Vitis vinifera grapevines against the ambient R. H. show large differences in curve shape. As indicated in Fig. 1j and k the Pp values increased (i.e. turgor pressure values decrease) with decreasing R.H. However, whereas the Pp values of the non-irrigated grapevines assumed a plateau value (corresponding to a constant turgor pressure) below about 50% R.H. (sigmoid curve shape, found in 83% of 30 R.H. plots), the Pp values of the well-watered grapevines increased dramatically when R.H. dropped further below 45 - 55% around noon (found in 90% of 31 R.H. plots). A sigmoid-shaped dependency of the Pp - R.H. relationship was also found for mucilage plug-rich leaves of E. pilularis using both the leaf patch clamp pressure probe (data not shown) and the Scholander pressure bomb (see Fig. 5 in Zimmermann et al. 2007a). Both techniques measure changes in turgor pressure in the low pressure range as shown recently (Westhoff et al. 2009).
Further work is certainly necessary to unravel the role of epistomatal mucilage plugs in more detail, but the results presented here demonstrate that the water binding capacity of acid mucopolysaccharides has to be taken into account in the discussion about water ascent and leaf water supply.
We are very grateful to Stefanie Nieft for performance of the microscopical cross-sections.
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