Protoplasma

, Volume 227, Issue 2–4, pp 113–118

Chromosome endoreduplication as a factor of salt adaptation in Sorghum bicolor

  • M. Ceccarelli
  • E. Santantonio
  • F. Marmottini
  • G. N. Amzallag
  • P. G. Cionini
Article

Summary.

Nuclear DNA amounts were measured by Feulgen cytophotometry in Sorghum bicolor cv. 610 plants early exposed to 150 mM NaCl, a treatment known to induce an increased tolerance to salinity in plants carrying this genotype. In salt-treated plants, the percentages of 8C, 16C, and 32C nuclei in roots in the primary state of growth were 21.9%, 13.3%, and 4.3%, respectively. By contrast, in nonsalinized plants, only 3.5% of the nuclei had an 8C content and no higher DNA contents were observed. The salt treatment induced chromosome endoreduplication during the differentiation of cells in the root cortex, where 41.2% of the cells displayed a DNA content higher than 4C (versus 1.3% in control plants). No enhancement of endopolyploidy was observed in cells of the root vascular cylinder or the leaves of the salt-treated plants. In another S. bicolor genotype (DK 34-Alabama), noncompetent for salt adaptation, the same NaCl treatment did not induce chromosome endoreduplication in root cortex cells. Endopolyploidy may be considered as a part of the adaptive response of S. bicolor competent genotypes to salinity.

Keywords: Chromosome endoreduplication; Feulgen cytophotometry; Root cortex cell; Salt adaptation; Sorghum bicolor

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • M. Ceccarelli
    • 1
  • E. Santantonio
    • 1
  • F. Marmottini
    • 2
  • G. N. Amzallag
    • 3
  • P. G. Cionini
    • 1
  1. 1.Dipartimento di Biologia Cellulare e AmbientaleUniversità di PerugiaPerugia
  2. 2.Dipartimento di Ingegneria CivileUniversità di PerugiaPerugia
  3. 3.Judea Center for Research and DevelopmentCarmel

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