Monatshefte für Chemie - Chemical Monthly

, Volume 150, Issue 3, pp 419–428 | Cite as

Reduction behavior of insecticide azoxystrobin and its voltammetric determination using silver solid amalgam electrode

  • Renáta ŠelešovskáEmail author
  • Marie Herynková
  • Jana Skopalová
  • Pavlína Kelíšková-Martinková
  • Lenka Janíková
  • Jaromíra Chýlková
Original Paper


Electrochemical reduction of systemic fungicide azoxystrobin, used to protect a wide variety of crops, was investigated using cyclic voltammetry with a mercury meniscus-modified silver solid amalgam electrode (m-AgSAE). The mechanism of the electrochemical reduction was proposed and supported with high-performance liquid chromatography/mass spectrometry analysis of azoxystrobin solutions electrolyzed on mercury pool electrode. An analytical method for the determination of azoxystrobin by differential pulse voltammetry with m-AgSAE was developed. A wide linear dynamic range (2.0 × 10−6–5.0 × 10−5 mol dm−3) and low limit of detection (7 × 10−7 mol dm−3) were obtained. The accuracy and repeatability of the proposed method were evaluated by the analysis of model solutions with recovery from 96.0 to 104.0% and relative standard deviation of 5 repeated determinations < 3.5%. Finally, a new voltammetric method using m-AgSAE was successfully applied for azoxystrobin determination in real samples, concretely in spiked river water and pesticide preparations.

Graphical abstract


Pesticides Azoxystrobin Reduction mechanism Voltammetric determination Silver solid amalgam electrode 



This work was supported by the grant project of The Czech Science Foundation (Project No. 17-03868S) by The University of Pardubice (Projects No. SGSFChT_2018_003 and SD373001/82/30350(2016)) and by Palacký University in Olomouc (Project IGA_PrF_2018_027).


  1. 1.
    Knight SC, Anthony VM, Brady AM, Greenland AJ, Heaney SP, Murray DC, Powell KA, Schulz MA, Spinks CA, Worthington PA, Youle D (1997) Annu Rev Phytopathol 35:349CrossRefGoogle Scholar
  2. 2.
    Gisi U, Sierotzki H, Cook A, McCaffery A (2002) Pest Manag Sci 5:859CrossRefGoogle Scholar
  3. 3.
    Han Y, Liu T, Wang J, Zhang Ch, Zhu L (2016) Pestic Biochem Phys 133:13CrossRefGoogle Scholar
  4. 4.
    Rodrigues ET, Moreno A, Mendes T, Palmeira C, Pardal MA (2015) Chemosphere 132:127CrossRefGoogle Scholar
  5. 5.
    Rodrigues ET, Lopes I, Pardal MA (2013) Environ Int 53:18CrossRefGoogle Scholar
  6. 6.
    Catala-Icardo M, Gomez-Benito C, Simo-Alfonso EF, Herrero-Martinez JM (2017) Anal Bioanal Chem 409:243CrossRefGoogle Scholar
  7. 7.
    Sundravadana S, Alice D, Samiyappan R, Kuttalam S (2008) J Braz Chem Soc 19:60CrossRefGoogle Scholar
  8. 8.
    Chonan T (2001) J Food Hyg Soc Jpn 42:249CrossRefGoogle Scholar
  9. 9.
    Li W, Wu YJ, Qin DM, Ma Y, Sun YJ, Qiu SP (2008) Chromatographia 67:761CrossRefGoogle Scholar
  10. 10.
    Giza I, Sztwiertnia U (2003) Acta Chromatogr 13:226Google Scholar
  11. 11.
    Hu D, Xu X, Cai T, Wang WY, Wu CJ, Ye LM (2017) J Food Protect 80:2112CrossRefGoogle Scholar
  12. 12.
    Noegrohati S, Hernadi E, Asviastuti S (2018) B Environ Contam Tox 100:821CrossRefGoogle Scholar
  13. 13.
    Pacheco WF, Doyle A, Duarte DRA, Ferraz CS, Farias PAM, Aucelio RQ (2010) Food Anal Meth 3:205CrossRefGoogle Scholar
  14. 14.
    Novotný L, Yosypchuk B (2000) Chem Listy 94:1118Google Scholar
  15. 15.
    Yosypchuk B, Barek J (2009) Crit Rev Anal Chem 39:189CrossRefGoogle Scholar
  16. 16.
    Fadrna R (2004) Anal Lett 37:3255CrossRefGoogle Scholar
  17. 17.
    Cizkova P, Navratil T, Sesakova I, Yosypchuk B (2007) Electroanalysis 19:161CrossRefGoogle Scholar
  18. 18.
    Selesovska R, Navratil T, Vlcek M (2007) Chem Anal (Warsaw) 52:911Google Scholar
  19. 19.
    Barek J, Fischer J, Navratil T, Peckova K, Yosypchuk B, Zima J (2007) Electroanalysis 19:2003CrossRefGoogle Scholar
  20. 20.
    Barek J, Pecková K, Vyskočil V (2008) Curr Anal Chem 4:242CrossRefGoogle Scholar
  21. 21.
    de Souza D, Melo LC, Correia AN, de Lima-Neto P (2011) Quim Nova 34:487CrossRefGoogle Scholar
  22. 22.
    Danhel A, Barek J (2011) Curr Org Chem 15:2957CrossRefGoogle Scholar
  23. 23.
    Selesovska-Fadrna R, Fojta M, Navratil T, Chylkova J (2007) Anal Chim Acta 582:344CrossRefGoogle Scholar
  24. 24.
    Yosypchuk B, Sestakova I, Novotny L (2003) Talanta 59:1253CrossRefGoogle Scholar
  25. 25.
    Juskova P, Ostatna V, Palecek E, Foret F (2010) Anal Chem 82:2690CrossRefGoogle Scholar
  26. 26.
    Kucharikova K, Novotny L, Yosypchuk B, Fojta M (2004) Electroanalysis 16:410CrossRefGoogle Scholar
  27. 27.
    Fadrna R, Yosypchuk B, Fojta M, Navrátil T, Novotný L (2004) Anal Lett 37:399CrossRefGoogle Scholar
  28. 28.
    Fadrna R, Cahova-Kucharikova K, Havran L, Yosypchuk B, Fojta M (2005) Electroanalysis 17:452CrossRefGoogle Scholar
  29. 29.
    Yosypchuk B, Fojta M, Havran L, Heyrovsky M, Palecek E (2006) Electroanalysis 18:186CrossRefGoogle Scholar
  30. 30.
    Janikova-Bandzuchova L, Selesovska R, Chylkova J, Nesnidalova V (2016) Anal Lett 49:19CrossRefGoogle Scholar
  31. 31.
    Janikova L, Selesovska R, Rogozinska M, Tomaskova M, Chylkova J (2016) Monatsh Chem 147:219CrossRefGoogle Scholar
  32. 32.
    Stepankova M, Selesovska R, Janikova-Bandzuchova L, Chylkova J (2015) Chem Listy 109:527Google Scholar
  33. 33.
    Fischer J, Hajkova A, Pereira M, Krecek M, Vyskocil V, Barek J (2016) Electrochim Acta 216:510CrossRefGoogle Scholar
  34. 34.
    Chorti P, Fischer J, Vyskocil V, Economou A, Barek J (2014) Electrochim Acta 140:5CrossRefGoogle Scholar
  35. 35.
    de Souza D, de Toledo RA, Galli A, Salazar-Banda GR, Silva MRC, Garbellini GS, Mazo LH, Avaca LA, Machado SAS (2007) Anal Bioanal Chem 387:2245CrossRefGoogle Scholar
  36. 36.
    Novotný L (1998) Fresenius J Anal Chem 362:184CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Environmental and Chemical Engineering, Faculty of Chemical TechnologyUniversity of PardubicePardubiceCzech Republic
  2. 2.Department of Analytical Chemistry, Faculty of SciencePalacký University in OlomoucOlomoucCzech Republic

Personalised recommendations