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Voltammetric behavior of a candidate anticancer drug roscovitine at carbon electrodes in aqueous buffers and a cell culture medium

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Abstract

Roscovitine is a candidate anticancer drug acting as an inhibitor of cyclin-dependent kinases which are involved in cell-cycle control and in apoptosis triggering. In our present study,we show for the first time that roscovitine yields a well-developed, analytically useful signal due to its irreversible electrochemical oxidation in alkaline range of pH (while in acidic media, its oxidation process is apparently rather complex and corresponding voltammetric signals are less suitable for analytical purposes). We demonstrate the possibility of voltammetric determination of the drug in buffer solutions (down to submicromolar concentrations of roscovitine) as well as in cell culture medium (down to micromolar concentrations) using in-house-fabricated pyrolytic graphite or screen-printed carbon electrodes.

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Acknowledgements

This research was supported by the Czech Science Foundation (project P206/12/G151). Support from the Academy of Sciences of the Czech Republic (RVO: 61388963) and Ministry of Education, Youth and Sports of the Czech Republic under project CEITEC 2020 (LQ1601) is acknowledged. The authors thank Metrohm CZ for efficient technical, material and intellectual support.

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Authors and Affiliations

  1. Institute of Biophysics, Czech Academy of Sciences, Kralovopolska 135, 612 65, Brno, Czech Republic

    Petr Orság, Luděk Havran, Lukáš Fojt, Jan Coufal, Václav Brázda & Miroslav Fojta

  2. Central European Institute of Technology, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic

    Miroslav Fojta

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  1. Petr Orság
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  2. Luděk Havran
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  3. Lukáš Fojt
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  4. Jan Coufal
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  5. Václav Brázda
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Correspondence to Miroslav Fojta.

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Orság, P., Havran, L., Fojt, L. et al. Voltammetric behavior of a candidate anticancer drug roscovitine at carbon electrodes in aqueous buffers and a cell culture medium. Monatsh Chem 150, 461–467 (2019). https://doi.org/10.1007/s00706-018-2333-5

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  • DOI: https://doi.org/10.1007/s00706-018-2333-5

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