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Monatshefte für Chemie - Chemical Monthly

, Volume 149, Issue 9, pp 1709–1718 | Cite as

Isotachophoretic determination of amino acids after their conversion to hydroxy acids

  • Michaela Kovářová
  • Martin Bartoš
  • Tomáš Mikysek
  • Dana Hloušková
  • Blanka Hyhlíková
  • Jana Vladyková
Original Paper
  • 23 Downloads

Abstract

In this article, determination of amino acids by capillary isotachophoresis based on their derivatization (van Slyke reaction) is presented. Hence, the suggested method deals with reaction of amino acids with nitrous acid forming corresponding hydroxy acids. This reaction was used in isotachophoretic analysis for the first time. Optimal conditions represented by electrolyte system for separation of hydroxy acids, 0.01 mol/dm3 HCl, 0.05% hydroxyethyl cellulose, with β-alanine (leading electrolyte of pH 3.6) and 0.01 mol/dm3 valeric acid with sodium hydroxide (terminating electrolyte of pH 7.24), were chosen; furthermore, other critical details of the analysis are also described. Developed method brings original results and shows applicability on analysis of amino acids in relatively simple mixtures, especially in food supplements.

Graphical abstract

Keywords

Amines Diazo compounds Electrophoresis Zwitterions 

References

  1. 1.
    Jastrzebska A, Piasta AM, Szlyk E (2015) J Food Compos Anal 40:136CrossRefGoogle Scholar
  2. 2.
    Kvasnička F (1999) J Chromatogr A 838:191CrossRefPubMedGoogle Scholar
  3. 3.
    Kvasnička F, Krátká J (2006) Cent Eur J Chem 4:216Google Scholar
  4. 4.
    Everaerts FM, Beckers JL, Verheggen ThPEM (1976) Isotachophoresis: theory, instrumentation and applications. Elsevier, AmsterdamGoogle Scholar
  5. 5.
    Hirokawa T, Gojo T, Kiso Y (1986) J Chromatogr 52:415Google Scholar
  6. 6.
    Prest JE, Baldock SJ, Fielden PR, Goddard NJ, Treves Brown BJ (2004) J Chromatogr A 1051:221CrossRefPubMedGoogle Scholar
  7. 7.
    Hirokawa T, Taka T, Yokota Y, Kiso Y (1991) J Chromatogr 555:247CrossRefGoogle Scholar
  8. 8.
    Zgola-Grześkowiak A, Grześkowiak T (2012) Int J Food Prop 15:628CrossRefGoogle Scholar
  9. 9.
    Kler PA, Huhn C (2014) Anal Bioanal Chem 406:7163CrossRefPubMedGoogle Scholar
  10. 10.
    Everaerts FM, van den Put JM (1970) J Chromatogr 52:415CrossRefPubMedGoogle Scholar
  11. 11.
    Roach D, Gehrke CW (1969) J Chromatogr A 44:269CrossRefGoogle Scholar
  12. 12.
    Zhang X, Zhao T, Cheng T, Liu X, Zhang H (2012) J Chromatogr B 906:91CrossRefGoogle Scholar
  13. 13.
    Acquaviva A, Romero LM, Castells C, Ramis-Ramos G, Herrero-Martinez JM (2014) Anal Methods 6:5830CrossRefGoogle Scholar
  14. 14.
    Acquaviva A, Romero LM, Castells C (2016) Microchem J 129:29CrossRefGoogle Scholar
  15. 15.
    Akula KK, Chandrasekaran B, Kaur M, Kulkarni SK (2015) Acta Chromatogr 27:413CrossRefGoogle Scholar
  16. 16.
    Castellanos M, van Eendenburg CV, Gubern C, Sanchez JM (2016) J Chromatogr B 1029:137CrossRefGoogle Scholar
  17. 17.
    Sun Y, Xu X, Mou Z, Wang J, Tan Z, Wu S (2012) J Sep Sci 35:3421CrossRefPubMedGoogle Scholar
  18. 18.
    Peake RWA, Law T, Hoover PN, Gaewsky L, Shkreta A, Kellogg MD (2013) Clin Chim Acta 423:75CrossRefPubMedGoogle Scholar
  19. 19.
    van Slyke DD (1911) J Biol Chem 9:185Google Scholar
  20. 20.
    van Slyke DD (1912) J Biol Chem 12:275Google Scholar
  21. 21.
    Kainz G, Huber H (1959) Microchim Acta 47:51CrossRefGoogle Scholar
  22. 22.
    Kotrlý S, Šůcha L (1988) Chemical balances in analytical chemistry (Czech version). SNTL, PragueGoogle Scholar
  23. 23.
    Kuramoto N (1986) Chem Express 1:343Google Scholar
  24. 24.
    Tazaki M, Hayashita T, Fujino Y, Takagi M (1986) Bull Chem Soc Jpn 59:3459CrossRefGoogle Scholar
  25. 25.
    Zaugg S, Caslavska J, Theurillant R, Thorman W (1999) J Chromatogr A 838:237CrossRefPubMedGoogle Scholar
  26. 26.
    Blatný P, Kvasnička F, Kendler E (1996) J Chromatogr A 737:255CrossRefGoogle Scholar
  27. 27.
    Danková M, Kaniansky D, Fanali S, Ivanyi F (1999) J Chromatogr A 838:31CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Michaela Kovářová
    • 1
  • Martin Bartoš
    • 1
  • Tomáš Mikysek
    • 1
  • Dana Hloušková
    • 1
  • Blanka Hyhlíková
    • 1
  • Jana Vladyková
    • 1
  1. 1.Institute of Analytical Chemistry, University of PardubicePardubiceCzech Republic

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