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Monatshefte für Chemie - Chemical Monthly

, Volume 149, Issue 6, pp 1003–1008 | Cite as

An NBD–NH2 fluorescent probe for bioimaging: existence of a specific detection of ClO

  • Yunxia Yang
  • Di Zhang
  • Mengsheng Xu
  • Jianhong Wang
  • Jipeng Chen
  • Le Wang
Original Paper
  • 225 Downloads

Abstract

A fluorescent probe 4-amino-7-nitrobenz-2-oxa-1,3-diazole NBD–NH2 for critical sensing hypochlorous (ClO) has been developed in this paper. Probe NBD–NH2 exhibited excellent sensitivity and high selectivity toward detection of ClO in CH3CH2OH/HEPES solution (1:4, v/v, 10 mM, pH 7.4), where the detection limit as low as 40 nM. The green fluorescence of NBD–NH2 was distinctly quenched by addition of ClO. In addition, the new probe was also applied for bioimaging using HeLa cells and exist a critical detection of ClO in living cells.

Graphical abstract

Keywords

NBD–NH2 Fluorescent probe Hypochlorous 

Notes

Acknowledgements

The authors thank Professor Zhihua Sun and Li-June Ming for carefully reading the manuscript and for constructive comments. We are grateful for financial support from the Natural Science Foundation of Shanghai (No. 17ZR1412000) and the Scientific and Technological Project of Henan Province of China (162102310352).

Supplementary material

706_2018_2143_MOESM1_ESM.doc (218 kb)
Supplementary material 1 (DOC 218 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Yunxia Yang
    • 1
  • Di Zhang
    • 2
  • Mengsheng Xu
    • 1
  • Jianhong Wang
    • 1
  • Jipeng Chen
    • 1
  • Le Wang
    • 1
  1. 1.College of Chemistry and Chemical EngineeringShanghai University of Engineering ScienceShanghaiChina
  2. 2.Henan Cereal Quality and Safety Testing Key Laboratory, Institute of Quality Standards and Testing Technology for Agro-ProductsHenan Academy of Agricultural SciencesZhengzhouChina

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