An NBD–NH2 fluorescent probe for bioimaging: existence of a specific detection of ClO−
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A fluorescent probe 4-amino-7-nitrobenz-2-oxa-1,3-diazole NBD–NH2 for critical sensing hypochlorous (ClO−) has been developed in this paper. Probe NBD–NH2 exhibited excellent sensitivity and high selectivity toward detection of ClO− in CH3CH2OH/HEPES solution (1:4, v/v, 10 mM, pH 7.4), where the detection limit as low as 40 nM. The green fluorescence of NBD–NH2 was distinctly quenched by addition of ClO−. In addition, the new probe was also applied for bioimaging using HeLa cells and exist a critical detection of ClO− in living cells.
KeywordsNBD–NH2 Fluorescent probe Hypochlorous
The authors thank Professor Zhihua Sun and Li-June Ming for carefully reading the manuscript and for constructive comments. We are grateful for financial support from the Natural Science Foundation of Shanghai (No. 17ZR1412000) and the Scientific and Technological Project of Henan Province of China (162102310352).