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Archives of Virology

, Volume 146, Issue 1, pp 71–86 | Cite as

Additional Rep-encoding DNAs associated with banana bunchy top virus

  • C. L. Horser
  • M. Karan
  • R. M. Harding
  • J. L. Dale

Summary.

 Banana bunchy top nanovirus (BBTV) has a multicomponent circular single-stranded DNA (cssDNA) genome consisting of at least six components. We have cloned, sequenced and analysed two additional cssDNA components, designated BBTV-S1 and S2, associated with a Taiwanese BBTV isolate. The sequences of BBTV-S1 and S2 comprised 1109 and 1095 nucleotides (nt), respectively, and like BBTV DNA-1, potentially encoded replication initiation proteins (Reps). However, the genome organisation of BBTV-S1 and S2 differed from that of BBTV DNA-1 in that (i) the stem sequence of the CR-SL was not conserved, (ii) the internal gene was absent and (iii) the probable TATA boxes were located 5′ of the stem-loop. Further, sequence and phylogenetic analysis of the Rep genes indicated that BBTV DNA-S1 and S2 were distinct from BBTV DNA-1. When different geographical isolates of BBTV were tested for the presence of BBTV-S1/S2, these components were detected in various isolates from Vietnam, Taiwan, the Philippines, Tonga and Samoa but were not detected in isolates from Australia, Egypt, Fiji, and India. Based on these results, BBTV-S1 and S2 do not appear to be integral components of the BBTV genome and represent additional Rep-encoding DNAs associated with BBTV.

Keywords

Nucleotide Phylogenetic Analysis Internal Gene Genome Organisation Integral Component 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 2001

Authors and Affiliations

  • C. L. Horser
    • 1
  • M. Karan
    • 1
  • R. M. Harding
    • 1
  • J. L. Dale
    • 1
  1. 1.Centre for Molecular Biotechnology, Queensland University of Technology, Brisbane, Queensland, AustraliaAU

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