Archives of Virology

, Volume 146, Issue 2, pp 379–387 | Cite as

Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus

  • E. Sato
  • T. Miyazawa
  • Y. Nishimura
  • Y. Ikeda
  • K. Kawakami
  • M. Mochizuki
  • N. Yokoyama
  • K. Maeda
  • K. Fujita
  • M. Kohmoto
  • E. Takahashi
  • T. Mikami
Brief Report
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Summary.

 We have previously reported the construction of a recombinant feline herpesvirus type 1 (FHV-1), designated C7301ddlTK-gag, expressing the Gag precursor protein of feline immunodeficiency virus (FIV). In this study, we report the construction of a further recombinant FHV-1 (ddlTK(gBp)-gag) which carries an FHV-1 gB promoter sequence upstream of the FIV gag gene of C7301ddlTK-gag. Strong expression of the FIV Gag protein by ddlTK(gBp)-gag was confirmed by immunoblot analyses and enzyme-linked immunosorbent assays. Although C7301ddlTK-gag and ddlTK(gBp)-gag failed to induce anti-FIV Gag antibodies in cats, we confirmed the infectivity and stability of these recombinants in cats.

Keywords

Immunosorbent Assay Immunoblot Analysis Promoter Sequence Precursor Protein Strong Expression 
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Copyright information

© Springer-Verlag/Wien 2001

Authors and Affiliations

  • E. Sato
    • 1
  • T. Miyazawa
    • 1
  • Y. Nishimura
    • 1
  • Y. Ikeda
    • 1
  • K. Kawakami
    • 2
  • M. Mochizuki
    • 2
  • N. Yokoyama
    • 3
  • K. Maeda
    • 4
  • K. Fujita
    • 1
  • M. Kohmoto
    • 5
  • E. Takahashi
    • 1
  • T. Mikami
    • 6
  1. 1. Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, JapanJP
  2. 2. Tsukuba Central Laboratories, Kyoritsu Shoji Co., Ltd., Ibaraki, JapanJP
  3. 3. Laboratory of Viral Oncology, Aichi Cancer Center Research Institute, Aichi, JapanJP
  4. 4. Department of Microbiology, Faculty of Agriculture, Yamaguchi University, Yamaguchi, JapanJP
  5. 5. National Institute of Animal Health, Ibaraki, JapanJP
  6. 6. The National Research Center for Protozoan Disease, Obihiro University, Hokkaido, JapanJP

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