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Archives of Virology

, Volume 146, Issue 2, pp 357–368 | Cite as

Partial characterisation of citrus leaf blotch virus, a new virus from Nagami kumquat

  • L. Galipienso
  • M. C. Vives
  • P. Moreno
  • R. G. Milne
  • L. Navarro
  • J. Guerri

Summary.

 Citrus leaf blotch virus (CLBV) was purified from leaves of Nagami kumquat SRA-153 that showed bud union crease when propagated on Troyer citrange. Virions were filamentous particles (960 × 14 nm) containing a 42 kDa protein and a single-stranded RNA (ssRNA) of about 9,000 nt (Mr 3 × 106). Infected tissue contained three species of double-stranded RNA (dsRNA) of Mr 6, 4.5 and 3.4 ×  106. The nucleotide sequence of several complementary DNA (cDNA) clones showed significant similarities with replication-related proteins from plant filamentous viruses in several genera. A digoxigenin-labelled probe from one of these cDNA clones hybridised in Northern blots with ssRNA from virions and with the three dsRNA species, suggesting that the ssRNA is the genomic RNA of the virus, the largest dsRNA is its replicative form, and the two smaller dsRNAs probably replicative forms of 5′ co-terminal subgenomic RNAs. CLBV was also detected in several citrus cultivars from Spain and Japan including Navelina sweet orange field trees propagated on Troyer citrange showing bud union crease; however, no virus could be detected in other citrus trees with similar symptoms. This indicates that CLBV is not restricted to kumquat SRA-153, but its involvement in causing the bud union disorder remains unclear.

Keywords

Sweet Orange Citrus Tree Replicative Form Subgenomic RNAs Citrus Cultivar 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 2001

Authors and Affiliations

  • L. Galipienso
    • 1
  • M. C. Vives
    • 1
  • P. Moreno
    • 1
  • R. G. Milne
    • 2
  • L. Navarro
    • 1
  • J. Guerri
    • 1
  1. 1. Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Valencia, SpainES
  2. 2. Istituto di Fitovirologia Applicata (CNR), Torino, ItalyIT

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