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Archives of Virology

, Volume 146, Issue 4, pp 757–766 | Cite as

A universal PCR primer to detect members of the Potyviridae and its use to examine the taxonomic status of several members of the family

  • J. Chen
  • J. Chen
  • M. J. Adams

Summary.

 A universal primer (Sprimer: 5-GGX AAY AAY AGY GGX CAZ CC-3, X = A, G, C or T; Y = T or C; Z = A or G), designed from the consensus sequences that code for the conserved sequence GNNSGQP in the NIb region of members of the family Potyviridae, was used to amplify by RT-PCR the 3′-terminal genome regions from infected plant samples representing 21 different viruses in the family. Sequencing of some of the fragments (c. 1.7 kb) showed that the type strain (ATTC PV-107) of Oat necrotic mottle virus is not a distinct species in the genus Rymovirus, but is synonymous with Brome streak mosaic virus (genus Tritimovirus) and that Celery mosaic virus is a distinct member of the genus Potyvirus not closely related to any other sequenced species. Potyviruses infecting crops in China were also investigated, showing that viruses on cowpea and maize in Hangzhou, Zhejiang province were respectively Bean common mosaic virus and Sugarcane mosaic virus and that one on garlic in Nanjing, Jiangsu province was Onion yellow dwarf virus. Fragments were also sequenced from Chinese isolates of Lettuce mosaic virus and Soybean mosaic virus (from Hangzhou), Turnip mosaic virus (2 different isolates from Zhejiang province) and RNA1 of Wheat yellow mosaic virus (from Rongcheng, Shandong province).

Keywords

Mosaic Virus Dwarf Virus Yellow Dwarf Yellow Mosaic Virus Soybean Mosaic Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 2001

Authors and Affiliations

  • J. Chen
    • 1
  • J. Chen
    • 2
  • M. J. Adams
    • 3
  1. 1. College of Life Science, Zhejiang University, Hangzhou, People’s Republic of ChinaCN
  2. 2. Virology Department, Zhejiang Academy of Agricultural Sciences, Hangzhou, People’s Republic of ChinaCN
  3. 3. Plant Pathology Department, IACR-Rothamsted, Harpenden, Hertshire, U.K.GB

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