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Archives of Virology

, Volume 146, Issue 6, pp 1075–1088 | Cite as

The relative infectivities and genomic characterisation of three distinct mastreviruses from South Africa

  • W. H. Schnippenkoetter
  • D. P. Martin
  • F. L. Hughes
  • M. Fyvie
  • J. A. Willment
  • D. James
  • M. B. von Wechmar
  • E. P. Rybicki

Summary.

 The genomic nucleotide sequences of the cloned agroinfectious genomes of three South African mastreviruses obtained from Zea mays, a Setaria sp., and Panicum maximum (designated MSV-Kom, MSV-Set, and PanSV-Kar respectively), were determined. Additionally, their relative infectivities and virulence were analysed in a range of differentially susceptible wheat, maize, and barley genotypes. MSV-Kom produced moderate to severe streak symptoms in all maize genotypes tested, but only moderate to very mild symptoms in the wheat and barley genotypes. MSV-Set infected only the susceptible to tolerant maize genotypes, but was generally more severe in the barley and wheat genotypes than MSV-Kom. PanSV-Kar was incapable of infecting any of the wheat and barley genotypes and only produced very mild symptoms on the three most sensitive maize genotypes. Genomic characteristics in common with related mastreviruses were identified. Phylogenetic analysis indicated that while MSV-Kom was closely related to previously sequenced MSV isolates, MSV-Set and PanSV-Kar represented distinctly novel strains of MSV and PanSV respectively. In the case of MSV-Set, this is the most distantly related MSV strain yet characterised.

Keywords

Nucleotide Phylogenetic Analysis Nucleotide Sequence Mild Symptom Wheat Genotype 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 2001

Authors and Affiliations

  • W. H. Schnippenkoetter
    • 1
  • D. P. Martin
    • 1
  • F. L. Hughes
    • 1
  • M. Fyvie
    • 1
  • J. A. Willment
    • 1
  • D. James
    • 1
  • M. B. von Wechmar
    • 1
  • E. P. Rybicki
    • 1
  1. 1.Department of Microbiology, University of Cape Town, Private Bag, Rondebosch, Western Cape, South AfricaZA

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